How to create an Adiabatic, Isochloric and Isobaric Environment?

I am trying to make an chamber to examine the growth of a micro organism in the above condition. The chamber temp. should be maintained at 95-100 C and. The environment should be tends to vacuum(Vacuum condition is practically impossible). Please help in choosing the material and conditioning the same.

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dashinfoline (author) 5 years ago
Thank you friends for showing your interest and helping me out in making my project.

Please help me more precisely in choosing the chamber material.
It is the main thing which is stooping to proceed ahead.
I would suggest using a borosil glass cylinder( they are designed to withstand all kinds of temperatures and pressures ). Moreover they are fully transparent. If transparency is not needed then stainless steel or aluminium is good( because it does not rust). The chamber should have a bottom section filled with water which can be heated( to vary humidity) and the entire thing must be placed in a thermos flask to help maintain the conditions.
Metal. Preferably stainless.

How big does it need to be ?

Steve
A chemist says glass, an engineer says metal...

L
True.

But surely a chemist who wanted to grow stuff in a giant box would go for metal.....
I know people who grow things in giant boxes... Mind you, that's not at low pressure and 100oC. The reference to examining things suggests this would be experimental rather than production to me.

L
dashinfoline (author)  lemonie5 years ago
This is purely meant for observation of micro organisms in extreme conditions
I know, get 'em sealed in ampoules and heat them with a steam-bath.

L
dashinfoline (author)  lemonie5 years ago
Steam Bath is the best option to heat the chamber externally in a controlled way.
I am going to use it.
We made a convector oven around ours - a big circulating fan, and a heater coil.
Unless you're below sea-level or under a high-pressure weather phenomenon you can't top 100oC and you're steady with the atmospheric-pressure at the time.
Last time I measured boiling water it was 98, but I'm getting towards 1,000 ft up.

L
dashinfoline (author)  steveastrouk5 years ago
It is an cylindrical chamber of diameter 20cm and height 20cm.
Vacuum Chamber.png
Try a glass dessicator jar. They'll hold a good vacuum, with an 'O' ring laid on the joint.

Steve
iceng5 years ago
Nalgene
dashinfoline (author) 5 years ago
Please give me some more details on choosing the material of chamber.
I am going to use LM36 as temp. sensor, a pressure sensor and a pH sensor.
lemonie5 years ago
Get a glass-blower to seal em in ampoules. If you freeze them in liquid nitrogen first and pump out the air you'll get your least gas-environment.
You need a lot of kit for this, obviously.

L