Question on plant cell life?

Hey,
As someone who wants to try micropropagation I would like to know how long plant cells can live when cut from the main tissue that they're found? And if the liquid that they're suspended in and the nutrients really make the difference can I be advised as to what solution to make up for transport?

Any help would be great,
Thanks,
Tom

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blkhawk4 years ago
Did you search the internet before you post your question? If you search Wikipedia the term micropropagation you will find your answer:


This small portion of plant tissue, sometimes only a single cell, is placed on a growth medium, typically containing sucrose as an energy source and one or more plant growth regulators (plant hormones). Usually the medium is thickened with agar to create a gel which supports the explant during growth. Some plants are easily grown on simple media, but others require more complicated media for successful growth; the plant tissue grows and differentiates into new tissues depending on the medium. For example, media containing cytokinins are used to create branched shoots from plant buds.

thomas9666 (author)  blkhawk4 years ago
I have searched the internet, and I know about the growth culture and how that affects the growth of cells. That's not what I'm asking. I'm asking how long the cells will live, and be able to grow, when disconnected from the main tissue and taken out of the transpiration stream. And what the standard transport solution they should be in to ensure that they survive longest
blkhawk's answer is correct. To get a more specific estimate of survival time, you need to specify what plant you will be working with. Google plant cell culture - find companies that sell supplies for plant tissue culture and ask for a catalog.
Do you mean like cuttings for cloning?

Or some other process.

By the way the plant is crucial as its natural longevity applies to how long it will survive under any condition.

Joe
thomas9666 (author)  Josehf Murchison4 years ago
It would be cuttings to culture from very little plant matter, but not in the traditional cutting method. On a sterile nutrient agar plate, even as little as one cell is placed, which grows into a mass of undifferentiated cells. Then the differentiation of the cells is regulated by the use of hormones and it grows into a fully functional plant.