"HC Off-Line Occured" while Interface Sysmex Xp100 machine using serial cable

We are Interface Sysmex XP100 Hematology machine, but we got "HC Off-Line Occured" error.

We are using Serial Port 9-pin female pins (2-->3, 3-->2, 5-->5)

Our Code To Open Serial Port:
SerialPort comport = new SerialPort();
comport.BaudRate = int.Parse(cmbBaudRate.Text);
comport.DataBits = int.Parse(cmbDataBits.Text);
comport.StopBits = (StopBits)Enum.Parse(typeof(StopBits), cmbStopBits.Text);
comport.Parity = (Parity)Enum.Parse(typeof(Parity), cmbParity.Text);
comport.PortName = cmbPortName.Text;
comport.Open();

Port successfully open, but when XP100 machine send output, it shows "HC Off-Line Occured" error.

Please provide possible solution.

Did you try Google?
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And if Google can't help you there is always the manufactures support line - which would be the most obvious choice...