Despite its exotic-sounding name, DNA is ubiquitous - it can be found in every cell of every living thing and almost everywhere on the planet. Nonetheless, we rarely come face-to-face with the molecule itself - and it's not because DNA is difficult to find or isolate! In this instructable, we'll show you how to isolate your own DNA with little more than some dish soap, table salt, high-proof alcohol, a shot glass, and a bit of your own saliva.

It only takes a couple of minutes, and after you've isolated your own DNA, you can either drink it back down in a tasty "DNA shot" (great party trick) or better yet, purify it further for more analysis*.

Materials & Set Up
  • 1/4 of a shot glass full of your saliva
  • several drops of dish soap (look for sodium laurel sulfate in the ingredients)
  • a pinch of table salt (1/16 of a teaspoon)
  • some contact-lens cleaning solution, meat tenderizer, or pineapple juice (optional)
  • Ice-cold 120-proof+ liquor (overproof rum works well)
The chemicals used in this experiment are "everyday" household items and are not particularly dangerous. Nonetheless, exercise extra caution and think twice if you decide to consume your DNA shot and ABSOLUTELY do not substitue rubbing alcohol, isopropyl alcohol, or any other non-consumable alcohol for the overproof rum we used. Besides using "denatured' alcohol, the other potential safety concern is the dishsoap added to the mixture. A couple drops won't hurt you, but if you are concerned about it, feel free to leave it out.

This instructable was produced by DIYbio - an organization for amateur biotechnologists. Visit diybio.org for more information.

EDIT: Some DIYbioers are developing a simple gel box and a gel box on steroids. We should have some instructables put together for them before Dec 08. If you are interested in helping, please join the DIYbio google group!

Step 1: Salivation...GO!

1/4 of a shot glass of saliva is harder to produce than you might think! Work your tongue against your cheeks and teeth as you think of a big juicy grilled steak / tofu cube / dim sum, or Muffins / baked cookies . I had to spit about 5 times to fill the glass 1/4th full.

If you are making the DNA shot for someone else, be sure to let them know where the DNA came from.

About This Instructable


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Bio: I am passionate about amateur biotechnology and more generally what is called Citizen Science. After graduating with a degree in Biology from Davidson College, I ... More »
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