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5 minute DNA Extraction in a Shot Glass

Step 3Some protease...

some protease...
Now that we've busted open the cells, they've spilled their guts all over the place in our saliva solution. in this step we try and get rid of as much of the protein part of those guts as we can.

A protease is a type of enzyme that can break down other enzymes. Meat tenderizer, pineapple juice, and soft contact lens cleaning solution all contain (different) proteases. A tiny bit of any of those should reduce the amount of protein that precipitates out with our DNA later on.
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Author:macowell
I am passionate about amateur biotechnology and more generally what is called Citizen Science. After graduating with a degree in Biology from Davidson College, I worked at MIT coordinating the Intern...
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