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Step 6Taking photographs
To take pictures you must use the lens of the microscope.
LIGHTING
I always use Kohler ilumination for light microscopy, you must start by doing this even if you want to do dark field or phase contrast photos.
FOCUS
Focus should be made looking through the camera, it may be a little hard to focus since the texture on the focusing screen becomes very visible.
EXPOSURE
If you are using a film camera make sure the light meter works without a lens, if it doesn't try ISO 400 for 1/2" second with the lamp brightness of the microscope set to the highest possible.
If you are using a digital camera the light meter probably won't work with this adapter so the best shot is to make a first exposure using ISO 200 and 1". Then on the picture preview check the histogram (levels). If the levels are to the left than increase exposure time, if they are to the right than decrease them. The idea is to have as much as possible of the area with bars (see picture).
WHITE BALANCE
White balance must be set to "Tungsten Light" or "Auto". For film cameras a blue filter (80B @ Amazon.com or Ebay.com) must be placed on top of the microscope lamp.
SHOOT THE PICTURE
When you are ready to shoot make sure you do so by using the self timer or a remote control shutter release to avoid motion blur.
IT'S A GOOD IDEA TO BRACKET.
LIGHTING
I always use Kohler ilumination for light microscopy, you must start by doing this even if you want to do dark field or phase contrast photos.
FOCUS
Focus should be made looking through the camera, it may be a little hard to focus since the texture on the focusing screen becomes very visible.
EXPOSURE
If you are using a film camera make sure the light meter works without a lens, if it doesn't try ISO 400 for 1/2" second with the lamp brightness of the microscope set to the highest possible.
If you are using a digital camera the light meter probably won't work with this adapter so the best shot is to make a first exposure using ISO 200 and 1". Then on the picture preview check the histogram (levels). If the levels are to the left than increase exposure time, if they are to the right than decrease them. The idea is to have as much as possible of the area with bars (see picture).
WHITE BALANCE
White balance must be set to "Tungsten Light" or "Auto". For film cameras a blue filter (80B @ Amazon.com or Ebay.com) must be placed on top of the microscope lamp.
SHOOT THE PICTURE
When you are ready to shoot make sure you do so by using the self timer or a remote control shutter release to avoid motion blur.
IT'S A GOOD IDEA TO BRACKET.
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