Step 4: Sterlize the growth medium
- First, pour 500 mls water, preferably destilled, into the pan
- Next, place the closed jars with medium into the pan. CAUTION: do NOT close the lid of the jar tightly! Otherwise the jars may explode while cooking!
- Additionally: put a piece of sterilization indicator tape on the lid.
- Steam the jars for 20-25 minutes into the high pressure pan.