Black lights (formally, Ultraviolet or UV lights) are just fun.  Not only are they great for when you are in a trance, but they can be used to identify those questionably unidentifiable stains on your bf's or gf's clothes.  But the coolest thing is how certain colors or chromophores react with UV lights.  Whites and fluorophore compounds (chemical side chains responsible for fluorescence) are especially colorful under a good UV lamp or array of UV LEDs.

In this instructable, I'll show you how to create your own fluorescent compounds out of something you probably already have in your home.

Step 1: Gather Your Items

What's the first thing that comes to mind when thinking about fluorescent ... well, besides fluorescent lighting?  Fluorescent markers, that's what!  Otherwise going by "Hi Liters" or some such thing, these humble little buggers are just screaming to be used for something besides highlighting organic chemistry text books.  And we're going to listen to their plea.

Grab some highlighters from your desk, bureau, backpack, friend's locker, mom's credenza...you know, wherever.  They're lying all about if you really look for them, those unassuming little pearls hiding right in front of our piggish noses.  You can use the big fat ones or the slimmer bic-type highlighters, depending on how much fluorescent solution you want to make.  The slim versions are fine to make about 150mL to 200mL, depending on how much highlighter fluid is in the highlighter pen. The fat highlighters can make upwards of 500mL of fluorescent solution.

Now, go get 'em!

Step 2: You've Got to Get to the Marrow

For this step, break open your hilighters.  I've found some pop off easily from the rear (I should retype that sentence) while others offer a bit of a struggle.  For the overdoer in our crowds, grab your dremel and cut that sucker open.  That's what I do.  Any occasion to cut anything open I use the faithful dremel.  I like it so much I have three, just so I can cut three things open at once.  Seriously.

Anyway, check out some of the pictures. 

Once you have the pen broken open, cut open, or otherwise dissected, you should find a fibrous center shaft that contains the ink.  Be careful and don't squeeze it when pulling it out of the pen's shaft.  Be prepared to transfer it immediately into a reservoir of water.  Being a biochemist, I use 150mL autoclave bottles but you can get by with anything as long as it's watertight.  For the water, I use distilled water at ambient room temperature (~ 25 Celsius) but again, for the less anal retentive, you can get on well with just tap water.  Regarding temperature, I've tried warm water to increase the rate of transfer of ink into water, but something about my brain prefers ambient to cool water.  Don't use ice water, m'kay?

Step 3: Soak the Ink Shafts

You've got the fibrous ink shafts out, so drop it in your waiting 150mL or so container of H2O.  It's more prudent to use less water than more, as you can always dilute it later if it's too concentrated.  You know it's too concentrated because it won't fluoresce.  If this happens to you, just add more water to dilute the solution until a UV LED casts a solid shaft of light through the fluorescent solution and causes the rest of the fluid to fluoresce nicely.  You'll know it when you see it.

Soak those ink shafts for a while, at least until the fibrous shafts are blanched clean/white.  Personally, I soak for about a day or two, but you can probably get most of the ink out in several hours.  Try turning the bottle every now and then or agitating the solution -- not by calling it names or hiding its homework, but by shaking it gently back and forth.

When the shafts are blanched pull them out and check the concentration with a UV LED and proceed to dilute if necessary.  You probably won't need to if you use a thin highlighter.  I've found that even thick yellow highlighters make a very bold fluorescence in 150mL while the same concentration in another color doesn't fluoresce at all.  Try yellow, green, pink, blue, or any other color that you find and like. 

Tighten off your bottles and save them back as your fluorescent stock to use in fun and interesting LED/blacklight lamp projects.

Step 4: Some Ideas

You can fill bottles (like empty clear bottles, especially neat if antique or laboratory looking) with your fluorescent solution and just put them somewhere, like a stand or curio cabinet or the top of your cabinets next to your beer bottle collection.

Another idea is to take clear plastic tubing and hold one end with your thumb while you pipette or use an eye dropper to aloquot solution into the tube.  It's a tricky endeavour as putting in the solution displaces air, which wants to go out your thumb side.  Play around with it.  When you're satisfied with the filling, cap off both ends with hot glue.  Consider getting UV SMD LEDs and running magnet wire behind the tubing with your LEDs.  It looks fantastic.

A final idea is to get some sort of scintillation vial (that's chemistry talk for a small vial with a screw-on, watertight cap) and:
  1. drill two holes in the cap
  2. take a 5mm UV led and thread the leads through the holes
  3. seal off the metal with silicon glue of any variety (i use "tough as nails")
  4. seal off the cap inside
  5. fill up the vial with your favorite fluorescent solution
  6. screw on the lid, adding more solution until there's no bubble
  7. now connect 3-5V via a 120 ohm resistor to the LED
Voila!  You have an encapsulated fluorescent container with black light integrated that you can add to many projects (blink it from a 555 timer, pulse dim it with PWM from an MCU, etc).  I may post a project using them in a separate instructable later.

Step 5: Conclusion

I hope you've enjoyed this instructable.  It's easy and inexpensive fun for you and the kids (just make sure they don't drink it).  I hope I was able to share something new and fun with you.  As always, I am open to comments and suggestions on this or any of my instructables.

This is beast!!!!!! I needed a flourescent dye because I'm going to make a steampunk grenade and I was gonna put a clear bulp at the bottom with the dye in it and an led so if you pull the pin an let the spoon fly (it's a dummy pinapple grenade) it'll glow. 5 stars, great instructable!!!!
That sounds like a great idea! Write it up, I'd love to read how you do it. Sounds awesome. I'm a fan of steampunk. Thanks alot for the post!
<p>Hey hahahah thank u so much u helped me a lot. I have a problem with my previous research topic bc it's so hard to do and i did some browsing and i saw this. I created an account here just to thank u. THANK U VERY MUCH for posting this :))))))))))))</p>
<p>do you think there is anyway to use this idea to make a blood splatter pattern naked to the eye and then use a uv light to show it im trying to find a way to make my class project cool! thanks anyone have ideas you rock </p>
<p>Very cool instructable, thank you. I have a question and perhaps you can help me. Do you have any idea of what homemade fluorescent powder I can use to detect theft? We have had poaching on our game farm, and yesterday we found an animal still ensnared. I'd love to be able to put a powder on it's hair and see what comes from it.</p>
<p>Hi,</p><p>Thanks for the nice words. I'm not sure I understand what you're wanting to do with the fluorescing substance. Are you thinking something akin to the dye packs found in bank vaults?</p>
<p>hi do you need a light to make it glow or can you just turn off the lights and it will glow on its on like a glow stick</p>
<p>a glow stick produces its characteristic glowing through chemiluminescence and not fluorescence. remember, fluorescence immediately reemits absorbed light, so you need a light source.</p>
Sorry, I'm slightly confused. Once they've turned white and are removed, what do you do? Do you need UV lights, or do they just glow?
I used parfum bottles and cork caps to make it look more stylish. <br>When i turn them on it's awesome. <br> <br>Great instructable!!
That's awesome! Great idea!<br>Thanks for sharing!<br>-gian
Oddly enough, about a week ago, before I had read this, I did essentially the same thing for a UV lit water vortex, figuring some fluorescence would look neater than plain water. <br>I definitely like the ideas you have here, good job ^.~
Hi! <br>Sounds like you have a neat project on your hands...anything with &quot;vortex&quot; in its name has got to be pretty cool. :) <br> <br>Thanks a lot!
So people say that UV light kills bacteria. Assuming that your creation isn't strong enough to kill germs, what can I do to make the UV stronger....?<br>thanks
To get antimicrobial effects from a UV source, you need a UV source that emits at between 200 - 300nm, with 250-260 being optimal to break phosphodiester bonds in bacterial DNA. All of the UV LEDs I own, and have ever seen, have wavelengths starting around ~ 400nm. <br><br>You might have better luck looking for a UV fluorescent tube. Use with caution!<br><br>Good luck!
Where's a good place to buy these little bottles? Thanks for the very cool instructable.
Thanks for the nice words!<br><br>There are several places out on the Intertubes where you can get these little bottles (called &quot;scintillation vials&quot;) but here's a link from a science project supply store that has several types of bottles that could potentially be used.<br><br>http://www.sciplus.com/category.cfm/subsection/7<br><br>Good luck!
COOL!!! But I have a stupid question..... Will the LED blow if its in the water? <br>
I think it would most likey short the circuit. Use rubber cement on the holes through the cap and to cover any exposed metal leads of the LED that will actually be in the solution and you should be fine. I've had my UV LED in that container of fluorescent solution you see in the pics for almost a year now and it still works just fine.<br><br>Good luck!<br>
I used rubbing alcohol to suspand the foresent dye. I saw in the image a tube full of foresent dye and wanted to try it out, i used crafting rubber cement glue(E6000) That i found at a dollor store and tryied it out, but i keep getting leaks from the tube. when i poped it out the glue was tacky. I would suggest to people not to mix rubbing alcohol to any type of rubber hardner. <br><br>I am trying to dye the tube now with foresent dye. Alot of people online say to use 10% acetone to 40%water and remainder dye suspanded in water. So far the tube went from transparent to opaque and from clear to pinkish. I wonder if its cause the acetone is sperating the foresents from the dye and attatching to the tube.
the way you specheed was very funny, <br>i will try it for my cyber masks<br><br>greets
Thanks :] I just tried it ! looks very cool :] will use it as decor. in my room ^^
Haha, thanks, glad you liked it. :)
Well, I haven't actually started on it yet because I don't have everything I need. &nbsp;I need copper and brass paint and a feaux aging agent (kind like smearing black shoe polinsh on something and wiping it<br>Off so it sticks in the cracks and crevices. But I already ground the paint off so i need to paint it. I'm gonna paint the body copper and the spoon brass. &nbsp;I'm using a mini tobasco bottle for the bulb (you get them in MRE's. I don't think their available in any other country but I'm 14 so I wouldn't know.I fill that with the Fliourescent dye and epoxy that into the hole that came drilled in the bottom of the grenade after adding the circutry. And that consists of A UV led and a pressure swith that once pressure is releived, it completes the circuit. I'd put the switch by the spoon so once i let it fly, the uv led lights up the dye. &nbsp;Pretty simple I guess.&nbsp;
its gin or tonic water cant rember 1 of them also glows with the light so u could use that instead (cheaper)
yeah, it's probably the quinine in tonic water that's fluorescing.
Sounds like it'll be a great project. I look forward to reading it when you get it finished.<br><br>-gian<br><br>p.s. those mini-Tabasco bottles used to sell for hard cash in the field among US Marines. Anything to cover the taste of MRE's after months of eating them is worth gold. :)
Try this with Red, Green and Blue and make a Fluorescent RGB Mood Light! :)
You know, I laughed when I read that, but after thinking on it for a minute I think if instead of using water, you used an organic solvent, like sec-butyl-oh or methylene for two colors, you could separate them with a water-based solution, say red in octane, green in water, blue in octane. It should make a sort of plasma light, like a lava lamp. Groovy!
That would be pretty cool! :D
Hmmm, I remember extracting highlighter liquid back in grade 7 and 8. Never found a use for it though, and I certainly didn't have any UV LEDs! I'll certainly remember this trick should any of my projects require a large volume of fluorescing liquid!
Haha yeah, sort of in my mind in the same area as making slime and elbow macaroni pictures. But you never know when you might need some super duty fluorescing fluid! I got the video working showing the brightness and fluorescing you can produce with this method. Thanks for checking out my instructable!

About This Instructable




Bio: Gian is a computational biologist and is the Managing Director at Open Design Strategies, LLC. He holds a BA in Molecular/Cellular Biology and an ... More »
More by nevdull:Create A Custom Medieval-/Fantasy-Style Calligraphy Quill Practical DACs Using Enumerated Types as Bitflags 
Add instructable to: