It is important to state the fact that this is piece of equipment intended to be used in a Bio Safety Level 1 lab environment.
Also, it was conceived to keep a low oxygen level atmosphere inside of the chamber, it is constantly pumped with inert gas such as Nitrogen or Argon.
It is NOT(repeat...not) intended to keep organisms from going outside of the chamber, it is intended to keep a desired atmosphere inside, with positive pressure.
It is NOT air tight, the positive pressure and small leaks keeps outside air from going in.
This is a functional piece of biology lab equipment, it was welcomed inside GENSPACE, the first community Biolab, based in Brooklyn, New York.
Step 1: Chamber parts breakdown
1- Big plastic container (36 gal), as clear as possible, as big and square as you can find.
2- Gas intake
3- Relief valve
4- Double hatch system (two plastic food containers)
5- Double gasket with metal reinforcements
6- Pressure gauge
7- Rubber arms (flex tube) and gloves
8- C clamps
9- Insulation foam + silicon + duct tape
More detailed material list and building procedures ahead.
Step 2: Materials used
Step 3: Footers
I suggest using half inch screws.
I used 1/16 inch rubber to make small gaskets, reinforced with Lexel.
(!!!note on the Lexel: It takes very....very long to dry (curate), bu is is 1000x better than silicon!!!)