Step 7: Success!

So here's our first honest-to-god BioPrint... We loaded a liquid culture of E. coli +pGLO into the inkjet cartridge, modified the little "I <3 InkShield" demo Arduino program that came with the InkShield, and printed a couple of lines of "I <3 BioCurious" over and over on an agar plate. (I'll attach some code as soon as I figure out how to do that.) The agar plate was filled almost to the top, to minimize the printing distance.

As you can see, printing with live E. coli cells worked perfectly! We probably let the colonies develop a little too long, so the letters are a bit fuzzy. We get a spray of small colonies from stray cells - probably due to some sputtering from the inkjet head. We may be able to improve on that by adjusting the viscosity or cell density of the culture we load into the cartridge.

But overall, not bad for a first try!

After printing, we disinfected surfaces with bleach, rinsed out the ink reservoir with bleach, and then let some bleach drip-flush through the print head, followed with a lot of deionized water, to make sure no bleach would dry up inside the print head.

It would probably be a good idea to invest in a second hand ultrasonic jewelry cleaner, so you can sonicate the heck out of any dead cells or other deposits that may form inside the print head.
For printing in papers we use inks , but for priniting life cells what should we use ???????
Soften any 'plastic', abs with a couple drops. Mix by weight 60% lacquer thinner and 40% acetone. (You can alao combine 30 to 35% ether and 65 to 70% naphtha to make a petroleum distillate but remember it's 72% volatile!) Use a suitable eye dropper non-plastic or rubber...some plastics are safe as applicators. For really small precision jobs use a suitable syringe. Polyethylene for example should be ok to use. <br><br>(You know the drill for safely dealing with chemicals...don't be stupid! Get a little help from someone who knows what they're doing and where) (http://www.uic.edu/sph/glakes/harts1/HARTS_library/solventhazards.txt)<br>This solvent works fine for making plastic welds and all sorts of plastic repairs.<br><br>Make sure to use lacquer thinner and not just toluene. The additives help make a close solvent to weld plastic. Check VOC's before you start. No MEK required but it's ok if in the lacquer thinner. (Thin out some ABS solvent and dissolve ground abs to make a great filler. <br><br>
Sorry just fyi...I'm a hack so check everything before trying to open a cartridge. I have very advanced training in industrial fabrication but the tinkering is just a hobby that I totally get for no explained reason.<br>Keep safe.
<p>I'm absolutely amazed. This work is truly fantastic. No other words than compliments and compliments. A little question.<br>Do you think is it possible using living animal cells to build an active layer for an enzimatic bioreactor like a sintetic belly - gut?</p>
<p>Wow very cool</p>
<p>Good work with printer.</p>
<p>This is a fantastic Instructable. Now I won't have to steal live kidneys anymore.</p>
<p>kidneys and eggs yum!</p>
<p><iframe allowfullscreen="" frameborder="0" height="281" src="//www.youtube.com/embed/qXXZLoq2zFc" width="500"></iframe></p><p>The first minute explains the possibilities of BioPrinters.</p>
<p>Amazing work!!!</p>
<p>Three words: Living Litmus Paper</p>
<p>Aren't the inks in inkjet cartridges alcohol based? Wouldn't it make more sense to put rubbing alcohol to clean the cartridges rather than just plain water?</p>
<p>Wow! This is amazing. Great job. </p><p>Congratulations guys!</p>
People are selling refillable ink cartridges now, so it might be much easier (and safer) to buy a cartridge that is designed to be refilled. (Although, you'd still have to open it up &amp; remove the sponge) <br>http://www.amazon.com/s/ref=a9_asi_1?rh=i%3Aoffice-products%2Ck%3Arefillable+ink+cartridges&amp;keywords=refillable+ink+cartridges&amp;ie=UTF8&amp;qid=1443536137
<p>Awesome work..</p>
<p>it is seems to be fantastic.us it possible to get the coding for the bioprinter ? if yes plss mail me </p>
<p>This project really very cool!</p><p>Iam impressed.</p>
<p>Hi ! <br><br>Great project . is it possible to get the codes used for the project. im trying to figure out how to use inkshield for a similar project of mine and im only a beginner :)<br><br>Thanks alot !</p>
<p>How about using a cnc milling machine to cut a precision opening in the top of the ink cartridges. Then another part could be machined to fit in the opening to close it. Look at the Othermill - www.othermachine.co/othermill as a suggestion.</p>
<p>clever, i'll give this a go when they come to town!</p>
<p>hello, does anyone have a sample of the arduino code? please email me abrarnourallah@gmail.com</p>
<p>Excellent project. Must try this</p>
<p>Interesting! But I have a question. What happened to the plate of E.coli on the agar plate? Wouldn't the cells eventually die off or if given nutrients grow so that the words were obscured? Is there any way to preserve the cells besides a picture? Or is deterioration inevitable?</p>
<p>I am working on a presentation for College, does anyone know what the price is? I would also like to know what will the market for this be? Will it be distributed to hospitals or will it be able to be used in small clinics?</p>
<p>Hi Alex! I visited BioBots website! When will the BioBots be available?</p>
<p>We are working on something similar over at <a href="http://www.biobots.io/" rel="nofollow">BioBots</a>. A low cost, high resolution, 3D bioprinter that is revolutionizing the field of regenerative medicine.</p>
<p>Extremely good...!!</p>
<p>Its really good :)</p>
<p>Really good</p>
<p>Thats astounding...</p>
<p>Its magnificent :)</p>
<p>Very cool!!</p>
<p>Thats impressive</p>
<p><br>Thats impressive<br></p>
<p>Thats excellent</p>
<p><br><br>Its extremely good :)<br><br></p>
<p>Reading this Instructable gives me chills!</p>
<p><br>Thats extremely good...<br></p>
<p>Its really good :)</p>
<p><br>Very good...!!<br></p>
so awesome
<p>Wow, thank you so much for sharing, love reading about things like this. So interesting.</p>
<p>Awesome instructable! I'm actually constructing it for my Senior Project. What code did you use to run the printing? </p>

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