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Fluorescein is the main ingredient in the highlighter and it will glows magically under UV light when it is added into the water

When you prepare the fluorescein solution, sodium hydroxide is needed to make the fluorescein dissolve in the water (as fluorescein is not soluble well in water)

Note: please be carefully when deal with sodium hydroxide as it is highly corrosive

I had no problem with dissolving it without sodium hydroxide. I used distilled water. Just dumped the marker in the water and shook for a while. Still glowing brightly in UV after 5 years!

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