Step 5Administering the gas
Start with a small flow at the beginning. Too much too fast and the animal will panic and/or choke. Once the animal seems groggy and lays down, slowly increase the flow. At this point, the animal will appear to faint. Continue with the gas until the chamber is completely full with gas. Since CO2 is clear, you will have to judge when it is full. Wait two minutes or so and add more gas. At that point, the animal is dead, but sometimes it is hard to tell. Just let it sit for about five minutes or so. After that, shake the chamber and if the animal is not responsive, then it is dead.
Take care when removing the lid. Fan it slightly with the lid or something to prevent yourself from inhaling any gas. The animal will most likely have soiled itself and a layer of bedding will make for an easier cleanup.
When doing two or more animals at one time, such as a litter of mice, take care to not overcrowd the chamber as this will stress the animals and cause panic. Administer the gas as normal.
The most important part is not to stress the animals. When done properly, the animal dies very calmly and does not choke or panic.
At this point, the dead animal can either be fed to the snake or frozen for later.
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