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Grow Your Own Bioluminescent Algae

Step 2Preparation and Mixing

Preparation and Mixing
Sanitation is necessary so your batch doesn't crash. After you REALLY wash out the grow container, make sure there is absolutely no residue left. Some people say swirl some diluted bleach around. Others say to stick it in the microwave after it's completely dry (won't melt or deform if it's dry... wet is another story). Choose your preference.

Additionally, sanitize the tubing if you're using an air pump, and anything else you're using to prepare this batch.

Mix up a batch of salt water. Use purified water as tap water can contain chlorine or other things that might kill your batch.

  Mix the salt to a 1.019 specific gravity (sg) concentration. Directions on how to do this are on the back of the package... you'll need a hydrometer if you've never done it before.

Add in ~ 1 ml of the micro algae grow. In this case, less is more. The solution you received the culture in should already have enough nutrients to support sizable growth. If you don't want to mess with making your own solution (not necessarily a bad idea) many places that sell starter cultures will also sell culture solution.

Let both the solution and culture bag sit in the same area out of the sun for an hour or two. This is simply to let them reach room temperature. A sudden change in temp during transfer could shock the culture enough to significantly harm it. If your room temp is in the 70s (F) , you should be okay. Ideally, the water should be around 22 degrees Celsius.

Finally, transfer the algae into your bottles. Attached is a picture of a grower's setup. (Your bottles won't be green though)

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6 comments
Jun 10, 2010. 8:39 PMpyroman22 says:
but what do I do with the other things growing in my closet.....
Jun 17, 2010. 3:37 PMnewnoc says:
Ick...'tis gross! Seriously. XD
Jan 24, 2010. 12:21 PMocto137 says:
 can i just use saltwater from the ocean? that is where these come from, right?
Apr 2, 2009. 9:05 PMJohnMichael says:
(removed by author or community request)
May 1, 2009. 3:52 PMcodybj says:
HoodedRanger is correct; you need CO2, as these are photosynthetic algae.
Apr 28, 2009. 8:30 PMHoodedRanger says:
these are plants, so they need CO2. actually, scientists are trying to grow huge "fields" of algae and raise plankton to help control the CO2 levels of the atmosphere. i watch discovery alot .
Jan 15, 2010. 7:12 PMmiiwii3 says:
HA!!!!
I saw that too!
Feb 18, 2009. 1:54 AMjoshward35 says:
you guys might want to consider that dry ice gives of carbonic acid when mixed with the water. Just a thought.
Jun 20, 2008. 4:07 PMbenthekahn says:
What about using dry ice for CO2?
Jun 20, 2008. 5:19 PMbenthekahn says:
ya, I was thinking a little metal basket to hold the dry ice at the bottom of the tank.
Jun 20, 2008. 5:31 PMDungeonbrownies says:
i think itd make it wayyy too cold if anything., well, unless that is you manage to get it wrapped ina lot of layers of cheesecloth and put it in the corner or w/e

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