loading

Step 8: Incubation

Now that the hard part is over, you just need to let the jars rest somewhere warm and dark. Like in a cupboard above the refridgerator, or in a cardboard box by your computer tower.

This allows the spores to incubate into mycelium the body that absorbs nutrients and water.

Keep them around 80-86 degrees F. It will just take longer for the mycelium to grow if it isn't near the optimum temperature.

It takes around 3 to 4 days to see the first hairs of mycelium forming as white spots and 3 to 5 weeks to let the fungus get to a 100% foothold in all the substrate, depending on temperature conditions.
Help! I can't tell if this cake is contaminated, this is my first time trying to grow shrooms and I'm just not sure.
<p>toss it quick before it contaminates the rest of the cakes</p>
<p>i would say so I've seen cakes like that that didnt end up making it</p>
<p>I forgot to mention 200 ml of tap water is added too.</p>
<p>Here is an alternative where you make the following pudding agar.</p><p>For 200 ml</p><p>5 g of cocoa pudding powder.</p><p>5 g of agar</p><p>5 g of Diammonium phosphate</p><p>1g of Inositol</p><p>5 g of Potato starch</p><p>5 g Fructose</p><p>5 g Milk Powder</p><p>This medium turns into a gel pudding at 70 degrees C. </p><p>I am using it to grow Oyster mushrooms.</p>
<p>1 mole per L to zero moles HCl. Hydrochloric acid slowly evaporated outside. I did this also in a<strong> clean environment </strong>with 1 mole HCl. Normally after a week away from light and impurities no change in pH ( the ph was 1).</p><p>So I know that metals would work to be Bio remediated this way too since they are water soluble.</p>
<p>A bit off topic but I am using Penicillium. D which I isolated carefully on mold from a lemon. I am using the fungi to absorb toxins and trap them into the spores themselves. These toxins are gold, copper and nickel.</p><p>Once in my lab I had 1 mole of HCl measured plus pepsin with a pH meter. After a week this mold that I described above started to grow even though the pepsin will SOAKED in Hydrochloric acid. I measured the pH with paper and it was neutral. This fungi neutralized the acid!!!!!</p>
<p>Here is a picture of the done project.</p>
<p>I have discovered that if you boil a 1.2-1.5% aqueous solution of sodium dichloroisocyanurate with fresh garlic bulbs there MAY an advantage to adding both due to several reasons. Garlic is a stable compound if it is placed into medium like agar agar. The bleach with garlic is sterilizing and preventing the growth of potentially dangerous mold. </p><p>I was doing a project and I left garlic out and to my surprise no mold grew on it. There is naturally mold down there and if I could kill two birds with one stone all the better for the results.</p><p>I am testing that theory right now.</p>
<p>A 0.1% solution of sodium dichloroisocyanurate is less corrosive because it produces lye, organic cyuranic acid which lowers the ph from 10- 11 to 7-8. As all plants and fungi cannot survive ph 10- 11 and even 9 is pushing it a bit is more ideal. This is comparison to regular bleach. Also bleach is unstable and will breakdown and provide less protection against the pathogens. Salts of sodium dichloroisocyanurate are more stable.</p>
<p>If you are going to use my method BE VERY CAREFUL WITH the chemical <strong>Sodium dichloroisocyanurate.</strong> It is highly corrosive and poison and if mixed with a lot of water or just a bit of water it possibly produce Chlorine. chlorine monoxide, Nitrogen trichloride (extremely dangerous chemical), other dangerous nitrogen chlorine forming chemicals. Incorrect use of this chemical can result in fire, explosion or death!!!</p><p>To be more familiar with it always read the MSDS before using any chemical. If you accidently expose this chemical to WATER always quickly remove heat generated by flooding with at least 4 L of water!! Working with it recommended you use a gas mask as well in case of accident. </p>
<p>I will in the summer make a type of agar which contains agar, soya bean powder, potato starch, glucose, and solution of squat and solution of sodium dichloroisocyanurate. The squats and the dichloroisocyanurate are to deter the bacteria from growing as well as spores from growing on the plates.</p><p>If the squats and sodium dichloroisocyanurate chemicals are too high then the fungi (Oyster mushrooms) will not grow.</p>
<p>can i use rye flour cuz i cant find BRF in the stores where i live or do i need BRF For the growth to feed on ? </p>
<p>You can buy regular Brown rice, whole grain, and give it a pass through the cofee grinder or blender. It doesn't matter if its a bit chuncky. I did it and got 100% sucess (:</p>
Would these steps be similar for oyster mushrooms?
<p>I am wondering if I take spores from groceries will they grow on the material or do you have to get them from a company??</p>
<p>If you have a fresh mushroom you can try taking a spore print and using them but you are better off cloning. That is when you take a piece of tissue from the middle of the stem and put in on an agar petri dish and let it grow.</p>
<p>I SELL SYRINGES FOR 15$ DARK NOT WATER DOWN LIKE YOU FIND ON EBAY...2-3 DAY SHIPPING WITH TRACKING..THE SHROOM GUY..</p><p>THESHROOMGUY@HOTMAIL.COM</p>
contams can be a health hazard, some of them being very toxic. for example the black mold. if your jar is contaminated DO NOT OPEN IT, when you open a jar with mold in it, the spores go everywhere, and if inhaled in enough quantities, it will take you down. pressure cook the contaminated jars before opening to kill off the mold, then wear a mask and dispose of properly. i would just get rid of the whole thing, jar and all, your life is worth a lot more than a jar :)
not only worry about it being a safety hazard but , you have a chance of those free floating spores contaminating over and over making it so you cant grow your mushrooms... so throw it away do not open it.
<p>Open the jar outside and empty the contents. If you don't have a way to rinse it outside, put the lid back on, bring it back in and submerge it in a sink of hot soapy water and open the lid under the water. No need to waste jars by throwing them away.</p>
I have opened contaminated jars before and nothing happened. Although i would not recommend it because the spores can affect other things in your house, though this is also not very likely. <br><br>The jars can be used again if you are in need just use a pressure cooker to sterilize them. A warning though, there is the small chance that some unbelievably resistant spores may lurk in the jar, in which case your new medium will also be contaminated.<br><br>This is not likely and has never happened to me. People really make a bigger deal about it than it really is, but that is not to say that needless safety precautions are a bad thing. In fact they help you establish a succesfull work routine. But if you you really need the jar just reuse it, just make sure you dispose of the contaminants properly. (not anywhere near your house)
We don't want to spread fear mongering sensationalism. Not too likely your going to die from opening a contaminated jar buddy. Just like if your spaghetti sauce goes bad, it may stink but it's probably not deadly unless you eat a lot of it. True, you don't want to open it because it can spread the kind of spores or bacteria you don't want in your grow area. This can damage your success rate. Just throw it out. It's not any more dangerous than any spoiled kitchen item though.
when your fruiting them how long do you leave them in their for? and are they in the 8 oz jar or just dumped in there?<br />
If you want to start growing you should go to sites like the shroomery. People here will tell you things when they really have zero experience. Even in the instructable there is misinformation. Like cold shocking has been debunked, you keep the cakes in cold water while dunking cause it's harder for bacteria to grow in cold water. As for your question, birthing a cake(taking it put of the jar) at full colonization after a week of consolidation is typical. But you can fruit them in vitro also which means you leave the cakes in the jar and let fruit body's form. In vitro works fine but your fruits will be ugly and it's just not most people preferred method. And once you put them in the fruiting chamber you can expect to see growth in 3-10 days on average. Once they start growing they grow very fast. Each batch of mushrooms is called a flush. So the time in your fruiting chamber depends on how fast they are growing and how many flushes you get. Typically I see 2-5 flushes with my cakes. There is a lot of good info online about cultivating your own mushrooms but there's just as much bad. Your best bet is to stick with sites like the shroomery. Good spore suppliers are a must, don't gamble on suppliers that aren't sponsored by the shroomery. Some good ones are hawks eye, and the spore depot. My personal favorite is the spore depot. Anyway hope that answers your question and I hope my comment has made other people wiser to all the misinformation running rampant online. (Not spell checked or reread)
<p>Thank you for sharing this mushrooms growing PF-TEK method. For substrates lile perlite, vermiculite and brown rice flour i found all of them here <a href="http://www.magic-truffles-shop.com/sclerotia-cultivation/substrate.html" rel="nofollow">http://www.magic-truffles-shop.com/sclerotia-cultivation/substrate.html</a></p>
<p>you should put shake the spore syringe well in the steps</p>
By adding worm castings i believe you will get much better results. It is used in growing marijuanna for nutrients. I do not know about growing mushrooms, just adding my two cents.
That would be plain dumb...
<p>no its not, it is actually used with a combination with bee pollen to bring in the extra nutrients mushrooms need.</p>
<p>You're working in a sterile environment, and you're adding worm castings...<br>Seems to me that there are quite a lot bacteria and funghi spores in worm castings...<br>I once counted my wormcompost-tea in a lab and the average amount of colony forming units was 6 million / ml</p>
<p>Yes, well said that with spore syringes growing mushrooms are easy. I have also read important instruction on this website (<a href="http://www.magic-spores-shop.com/" rel="nofollow">http://www.magic-spores-shop.com/</a>) . </p>
Thanks for the info!
Very Nice,
I have an easier way. <br> <br>Step 1: Leave your house extremely dirty <br>Step 2: Mold should start growing in some places <br>Step 3: Continue monitoring your mold, and some mushrooms should start popping up <br> <br>Lol jk, but seriously, good idea man!
Does dunking it really make that big of a difference?? and at what temp. do u keep your fruiting chamber?
Heres your answer: <br>(first flush)
yes, it really does. try it for yourself, one with dunking and one without :)
hello great explication thank you but i am having the problem of find a spore bank that sends to portugal can you guys help me <br> <br>thank you
Great instrutable cant wait to try it out, any suggestions on where to get spores in the USA or Texas or Houston,<br>Thanks
Be careful not to get ripped off a lot of spore vendors on the net will do just that.<br>Use <a href="www.mushroomprints.com">Mushroom Prints</a> if you can, they are totally reliable and sometimes give away free spores and stuff with orders.
20 cool points to who ever made the video for using shpongle as the background music!
&nbsp;Instead of a pressure cooker, I used a large, deep pot, with lid, and just duct taped it to seal it up. &nbsp;Kind of white trash, but saves on the cooker.
Yes. This video describes another method for using just a pot instead of a pressure cooker: http://www.youtube.com/watch?v=ZHJQrsZFQdE&amp;feature=PlayList&amp;p=E242984A46FADF3E&amp;playnext_from=PL&amp;index=0&amp;playnext=1
Questions actually, how long until you picked mushrooms and per jar or number of jars how many mushrooms or pounds of mushrooms did you harvest?
I have heard that it is common to harvest a half ounce of mushrooms per cake, so if you're growing 10 cakes at once, you get 5 ounces.
&nbsp;That's about right... &nbsp;without dunking I had 12 jars, and after 1 flush I ended up with 5.5 oz of dried mushrooms. &nbsp;Had I dunked, it would probably be a lot more.

About This Instructable

319,328views

308favorites

License:

Bio: Twitter: @uberscientist
More by nak:MSP430 Accelerometer microSD Datalogger How to make a Filiberto's post card Homebrew C64 &quot;Paddle&quot; box 
Add instructable to: