This Is less project and more a good working practice or operating procedure for the necrosis of a frog. The frog depicted in this instructable (Xenopus laevis) is a pretty good muse as it has all the normal bells and or whistles, but in a larger format. Which makes it a LOT less fiddly than when preforming the procedure on smaller animals -- such as Xenopus tropicalis.
These animals where marked to be culled for several reasons. High on that list was poor egg production and age. The youngest one being ten years old. A very old frog, a much longer life span than any found in the wild. All these animals where used exclusively for breeding, we weren't trying different shades of blush to see which brought out their cheek bones. I personally cared for every aspect of their lives, as any good Animal Husband would. They were loved and culled in the most human and stressless way I possibly could.
And rather than cull then throw them in the rubbish, I used them to create this teaching tool. As well and allowing some of my work-study students the opportunity of first hand dissection. Which is rare in today's universities.
Step 1: Tools
The most basic kit is the best kit:
Scissors S/S (s/s stands for sharp / sharp. meaning it has two shape blades)
The other stuff
Dissection board (If you feel like it. When performing an necropsy, I just use a paper towel)
In the video, you will see the use of a razor blade. This is only because we couldn't find where I had hidden the snips. I did find them later.