This multipart instructable gives a step-by-step instructions on making an algae test photo bioreactor. This can be used in any application that calls for testing and/or growing algae, to determine maximum growth rates, best nutrients, etc., such as...
Making algae biodiesel,
Health food supplements and many more.
Because this is more involved than can be explained clearly in a single instructable, I'll have to include multiple parts.
You can also download a complete free eBook here:
It includes a complete parts list, color diagrams, step-by-step instructions, and full color photos.
Step 1: Cap Assembly...1
(10) 1.25 PVC threaded plugs
(10) Bulk Air stones
(20) 1/8 x � Barb Adapter
(1) Aquarium Silicone air hose
(10) �w Adaptors
(1) Tube 3M 5200 Marine Adhesive
(10) Check Valves
(10) 1.25 PVC Slip and Thread adapters
Drill press or power drill.
1/2" drill bit
9/16" deep socket
3M 5200 Marine adhesive
Assemble all the parts and tools necessary before beginning:
Step 2: Cap Assembly...2
A drill press works best of this as you can make extremely exact holes.
A regular hand drill will also work. Keep in mind if youre using a hand drill the caps will have a tendency to spin away from the drill. Youll need some sort of clamp or vice to keep the plug and drill bit steady.
Working in plastic and PVC, using a wood drill bit at medium speed and steady pressure seem to work best.
Step 3: Cap Assembly...3
Step 4: Cap Assembly...4
Step 5: Cap Assembly...5
Step 6: Cap Assembly...6
The hose barb should have approx. 3/8 of an inch extending from the bottom of the PVC cap. Put a small bead of adhesive around the threads.
Step 7: Cap Assembly...7
Step 8: Cap Assembly...8
Step 9: Cap Assembly...9
Step 10: Cap Assembly...10
Step 11: Cap Assembly...11
If pressing the air tube becomes difficult or kinks, place the end of the air tube into hot water for about a minute. This will soften the air tube and make it more malleable.
Step 12: Cap Assembly...12
Your cap assembly should now look like this:
Step 13: Cap Assembly...13
Cut 10- 1 inch pieces of tubing.
Step 14: Cap Assembly...14
Connect the one inch tubing to the barb extending from the cap.
Step 15: Cap Assembly...15
Step 16: Cap Assembly...16
Step 17: Cap Assembly...17
Step 18: Cap Assembly...18
TIP: Try blowing into the tube. You should be able to blow air all the way through. If obstruction, turn check valve around.
Step 19: Cap Assembly...19
Your cap assembly and one of the most difficult parts of your PBR construction is now complete.