Introduction: How to Use High Power Laser

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This guide is about how to use high power laser, especially the high power diode DPSS laser. These DPSS lasers are widely used in industrial and labs.

Step 1: Read the Laser Specifications

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Read the laser specifications , labels or manuals carefully, you need to know the laser wavelength, laser output power, laser colors. For most of the lasers, you can get the color by reading the wavelength. Red 660nm, green, 532nm, blue 473nm, blue violet 445nm, violet 405nm, infrared 808nm.

Step 2: Find Out Laser Switch

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Find out where is the laser switch or laser trigger. Make sure the laser is turned off before connecting the power supply.

Step 3: Connect TTL Modulation Line

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If you need TTL laser module, connect the TTL line

Step 4: Laser Safety Goggles

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Wearing laser safety goggles, according to the laser color. Different color lasers need different safety goggles.

Step 5: Point Laser Head Outside

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Make sure you point the laser head outside, don't point the laser to you or other human.

Step 6: Turn on Laser Switch

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Turn the laser on, for a visible laser, you can see the laser beam.


SHOE0007 made it! (author)2017-07-05

Beware of some cheap lasers that are poorly made. I make sure they have good reviews first and also make sure the power supply if used is not constant does not go past a specific value. Here are some laser art videos.

SHOE0007 (author)2016-10-29

Red laser with methylene blue for only 5-10 minutes of exposure was quite effective.

The methylene blue for the laser at 650 nm and red light (unknown Wavelength) with methylene blue as a

dye produced free radical oxygen.

Blue light even though the laser light was limited proved for blue light CFL for long hours to inhibit the growth

of this bacteria which I am testing.

An issue was tide, some detergents that blocked light from entering the cells of the bacteria. Long term use of laser and light sources

may produce bio fim that may block wavelengths from being photo toxic in effect.

The tide filtered with small amounts of purple lamp worked for 15 hour exposure on bacteria. Nutrients were added for more rapid growth.

Control grew and high concentration of tide not filtered is an issue in growth. Light may not penetrate the cells.

Electrical pulses at 7500 volts 20 ma for 5-10 minute exposures produced RFI that may interfere with bacteria growth. This method is expensive

and requires electrical training. I may test with 12000 volts 30 ma.

All growth for blue laser and red non pulsed and pulsed usually killed or inhibited the growth of bacteria. Maybe this method is only effective for

specific types of non sporing bacteria.

Oh UV disinefectant lamp at 254 without dyes or chemcials was super effective at tempary killing biofilm forming bacteria.

SHOE0007 (author)2016-09-22

Pulsed 100 mw line laser at 12.5 mw a second. Here is the data.

Yellow marker dye 0.5 ml of 4-5% in 100 ml tap water with bacillus
bacteria pulsed for 20 minutes (12.5 mw a sec) Growth: Negative. Extra 24 hours growth: Yes.

Blue tide solution with 100 ml tap water with bacillus
bacteria pulsed for 20 min. (Total exposure 12.5 mw per second.). Growth: Negative. Extra 24 hours: Yes.

Highly concentrated pink marker dye with sugar and bacteria
pulsed for 20 min (12.5 mw per second.). Growth: Negative. Extra 24 hours
growth: Yes.

Diluted pink marker in Tonic water with bacteria pulsed for
20 minutes (12.5 mw a second.). Growth: Negative. Extra 24 hours growth: Yes.

SHOE0007 (author)2016-09-21

Did more endospore staining and prooved that this bacteria is a harmless bacilius type.

SHOE0007 (author)2016-09-21

This unknown bacillius bacteria can be killed but the spores (Survive) for the purple laser properties. This is probably true for red laser (Methylene blue) experements as well.

SHOE0007 (author)2016-09-21

Here is more data on a purple laser line effecting growth of bacteria for 1 day. These unknown bacteria also tested positive for endospore bacteria. Rod gram positive bacteria.

Purple laser (Line) with bacteria (Quinine tonic water,
yellow marker dye) 15 minutes Growth: Negative. Growth for 24 hours extra. Yes.

Pink marker in 5% ethanol with glucose and bacteria under
100 mw purple laser line. 15 min. Growth: Negative. Yes for 24 hours extra.

Tide blue detergent solution in 100 ml water plus soil
bacteria and glucose. 15 min. Growth: Negative. Yes for growth 24 hours after.

Yellow marker dye in water with glucose and exposure level
15 minutes Growth: Negative. Yes.

JELLO (orange) 5 g in 100 ml with bacteria. 15 min exposure:
Negative. Growth for 24 hrs Yes.

Ammonia citrate 2.3 g in 200 ml. Aprox with 5 g glucose and
dyes. Exposure time 30 min. Growth: Negative. Growth for 24 hours extra: Yes.

SHOE0007 (author)2016-09-19

Here is more info on the 12 kV, 30 ma jacob ladder experiments with bacteria culture.

12 kV 30 ma transformer jacob ladder exposure to bacteria
for 10 minutes Growth: Negative. 5.35 uT

12 kV 30 ma transformer jacob ladder exposure 5 minutes
growth: Positive. 5.35 uT.

Notice that growth for 5 minutes but not for 10 minutes occurred. This level is harmful 11 times the safe background EM field but I am no were near it when it operates.

SHOE0007 (author)2016-09-16

The noraml growth for 1 hour in the middle was negative.

SHOE0007 (author)2016-09-16

Here is some more info on UV 254 nm light source. Some blue light is also given off which combines the sterilization effects.

UV 1 hour 254 nm with small concentration of yellow marker
dye: Growth in 20 hours: Positive. (Blocked UV light from passing into culture.)

Normal growth of unknown bacteria 1 hour No growth. (UV lamp
254 nm).

Tonic water with bacteria (exposed to 254 nm UV light) for 1
hour Growth: Negative.

Soap solution in bacteria culture. Exposure 254 nm 1 hour 20
hour growth: Negative.

SHOE0007 (author)2016-09-10

You should give info on Invisible lasers too. You cannot see the beam and requires a camera or such to see it!

SHOE0007 made it! (author)2016-09-10

Here is a set up of a working laser from e-bay. max 250 mw red 650 nm.

SHOE0007 (author)2016-09-10

Here is some data on info that I tested.


Growth of bacteria in methylene blue and red light:

Growth of bacteria under blue light: Negative.

Growth under black light and yellow marker dye: Positive

Growth under red cfl without chemicals: Negative.

1 day growth with spark gap (Jacob ladder) 15 minutes:

1 day growth of linden flask with cultures for 20 minutes: Negative.

2 days exposure of 532 nm 50 mw laser (20 minutes a day):

2 day with 1% pink marker dye 3.3 mw of 610 nm (total
exposure 532 nm):

2 day exposure to 99%
marker dye pink 29 mw 610 nm (green laser beam 42 mw 532 nw) laser pump:

Growth with 400 nm wavelength Tonic water: 20 hours
wavelength emittance 460 nm: Negative.

Growth with 400 nm with glucose and pink marker dye: Growth was negative.

Tide at 0.05 lumen. Kill bacteria?? Bacteria growth. Impurities
and cloudiness was a problem.

Pink marker dye with small amounts of ethanol and 405 nm 75
mw laser. Growth: Negative.

Bacteria in 0.5% C yellow marker dye in 100 ml water plus 75
mw 404 nm laser. Wavelength 512-520 nm. Growth: (30 min exposure): Negative.

Bacteria growth in 5% tide solution 1% yellow marker rest
water: Black lamp (Laser not working) 15 hour exposure: (Wavelength: 470 nm + 520 nm = two chem: 495
nm. Blue green color) Growth: negative.

Intense growth of bacteria in 100 ml with pink marker dye
solution (Wavelength > 532 nm): Growth.

400 nm intense source near filtered tide solution
concentration 5 g per 500 ml Growth: Positive.

650 nm approx. 180 mw into bac culture with yellow marker
dye 18 min Growth: Negative.

Telsa coil 15 minutes experiment Growth: Negative.

5 g glucose 100 ml solution with methylene blue and soil
bacteria exposed at 240 mw Growth: Negative. (18 min laser exposure), (20 hours
of growth).

Water and glucose plus soil bacteria at 240 mw red laser

Malachite green 1 drop into 100 ml with glucose and 1 g soil
growth with 240 mw red laser: Negative. (18 minute exposure Laser), (20 hours
of growth cycle).

Indigo carmine degraded 5 ml of 1.33% solution with glucose
and soil bacteria at 240 mw red laser Growth: Negative. (18 min exposure laser)
(20 hour exposure.)

Malachite green 1 drop (Control without) laser exposure:

Crystal violet 5 ml of 1% with bacteria and glucose solution
at 240 mw red laser. Growth: Negative (18 minute exposure and 20 hours of

Crystal violet 1 ml in 1% with 1.06 mg approx. of IBA
(Auxin) with soil bacteria and glucose and 240 mw red laser. Growth: Negative (18 minutes exposure laser and 20 hours

Methylene blue 1 ml 1% in 100 ml of IBA culture at 2.0 mg
with 10 minutes of exposure at 240 mw. (10 min laser), (20 hours exposure
growth.). Growth: Negative.

Malachite green 1 drop 2% with 2 ml of IBA. Friendly
bacteria growth for 20 hours with 10 min exposure to 240 mw. Growth: Negative.

SHOE0007 (author)2016-09-08

No growth of bacteria with yellow marker dye. 650 nm laser-- max wavelength is around 495 nm for this dye. Perhaps the laser energy was converted into heat and this plus the laser at 180 mw exposure killed the bacteria?

SHOE0007 (author)2016-09-07

Near 180 mw if at 250 mw power max.

SHOE0007 (author)2016-09-07

Here is 18 min for one day testing to see if yellow marker dye plus laser at 160 mw would work. To kill friendly bacteria or not??

SHOE0007 (author)2016-09-07

Here is a photo of the 650 nm at 160 mw (with rechargeable batteries).

SHOE0007 (author)2016-08-31

R2 is 1.

SHOE0007 (author)2016-08-31

Here is another one this time on pink marker dye solution.

efficency in water 0.067 out of 1.

SHOE0007 (author)2016-08-31

Here is more data on total W for 10 minutes if it is 75 mj a second (assume).

SHOE0007 (author)2016-08-31

Here is more data on the total milla lumen per hour for 20 hours. This is the total light emitted through the flask per hour.

SHOE0007 (author)2016-08-31

Blue light at 460 nm.

Lux = 54.3

Lux black lamp 460 lux.

54.3/460 lux *100% = 10% efficency. other materials can be lower like 1-5% in water.

SHOE0007 (author)2016-08-31

Here is a picture of tonic water which has approx 83 mg quinine per L. So for 100 ml there is 0.83 mg quinine. Here is the picture.

SHOE0007 (author)2016-08-30

Here is more data on red light with a higher lux output. It reads milli lux per hour for 15 hours.

SHOE0007 (author)2016-08-30

Here is some data on the 400 nm black lamp for 20 hours. Graph is the data time in hours verus lumens per meter per hour total.

SHOE0007 (author)2016-08-30

Here more data on 532 nm beam being exposed to dilute Pink marker dye at a rate of 3 mw a second.

SHOE0007 (author)2016-08-29

Data as graph.

SHOE0007 (author)2016-08-29

Data on green light exposure time to bacteria est 50 mw 532 nm.

0 min 0 watts.

1 min 3 Watts optical for 1 minute exposure (Pulsed by 555 timer).

2 min 6 Watts.

3 min 9 Watts.

4 min 12 W

5 min 15 W total

Above will be a graph for every 5 minute exposure. (Constant) for 5 minutes done 4 times daily.

SHOE0007 (author)2016-08-27

Here is a graph on that data.

SHOE0007 (author)2016-08-27

Here is an estimated Kv per cm total exposure to the bacteria.

Time min kV exposure total per cm

0 0

1 48

2 96

3 144

4 192

5 240

SHOE0007 (author)2016-08-27

Total exposure to 3 eposides of arcing of an jacob ladder for 25 hours killed the friendly unknown bacteria. Total of 15 min spread out 5 min 3 times.

SHOE0007 (author)2016-08-27

Here is a graph on minutes to 5 minutes and frequency of the pulses that the cultures are exposed to in Mega hertzs.

SHOE0007 (author)2016-08-27

Here is some data on 25000 hzs a second being pulsed through a jacob ladder. This is the frequency of the transformer.

0 minutes Pulses Hzs.

1 min 1.5 MHzs.

2 min 3.0 MHzs.

3 min 4.5 MHzs.

4 min 6.0 MHzs.

5 min 7.5 MHzs.

within 5 minutes the pulse goes to 0 to 7.5 MHzs.

SHOE0007 (author)2016-08-26

Here is another fast jacob ladder.

SHOE0007 (author)2016-08-26

Also 445 nm and 808 nm and other IR wavelengths can kill bacteria. Here is an article on 808 nm destroying bacteria.

SHOE0007 (author)2016-08-26

5 cm away from the pulse of 800 volts for 5 cm for 15 minutes for 1 day may or may not be sufficent to kill the friendly unknown bacteria. Soon my electromagnetic meter will be very useful in measuring the Volts per meter and the micro Telsa measurements. I will see tomorrow if this will work. Each few hrs for 3 times the jacob ladder was tested for 5 minutes.

SHOE0007 (author)2016-08-26

Also eventually I will test with a high voltage neon power supply (8 kV 20 ma) on bacteria to see if the bacteria can be killed or reduced with 4000 v per cm. 3 cm away would give a pulse of 1333.33 volts per cm for 3 cm away. I have made a jacob ladder.

SHOE0007 (author)2016-08-26

Here is qualitative data for 5 days. each day the light was on for 12 hours. After 2 days the bacteria (safe unknown) was completely destroyed by blue light.

Blue light exposure odor.

1 day 12 hrs. Normal no odor.

2 day 12 hrs. No odor.

3 day 12 hrs. No odor.

4 day 12 hrs. No odor.

5 day 12 hrs. No odor.

SHOE0007 (author)2016-08-20

Eventually from e-bay and other sites I will recieve a purple 405 nm (most intense source) of near UV. It is capable of inducing free radicals. How many oxygen molecules would it produce? That what I am testing on friendly bacteria for 6 -20 minutes.

405 nm 100 mw laser.

SHOE0007 (author)2016-08-20

Here is a picture of 5 g of glucose with a bacteria from (gram positive) with 100 ml of water. The exact amounts don't matter as long as it near 5 g. The compact blue light is being tested with this unknown bacteria to see if Free radical oxygen molecules are released. Tested each day with qualitative test with anaerobic bacteria. It is exposed to blue light 4-5 cm away for 12 hours for 5 days. Will it be capable of killing this type of bacteria?

SHOE0007 (author)2016-08-15

Another article see below talks about 500 mw per cm2 doses not with lasers but with a type of blue light capability to kill specific anaerobic pathogenic bacteria.

SHOE0007 (author)2016-08-15

Here is an article link of inducing metal free porphyrin that produces ROS (Reactive oxygen species) that induces free radical damage DNA, etc.

SHOE0007 (author)2016-08-15

Even high powered 200 mw 405 nm beam or blue 445 to 470 nm beam are capable of inducing photo toxic effects without any dyes. Yes they induce free radical molecules (I think oxygen) that acts and destroys DNA.

Very similar to methylene blue and the red pink beam at 660 nm.

Up to 1 W of optical power can be used or more but In my opinon this is over kill.

SHOE0007 (author)2016-08-14

Biofilm layer sediment, etc will play a role in the effectiveness of the photo dynamic effect of the laser hitting the dye.

SHOE0007 (author)2016-08-14

I was thinking that with methylene blue or other dyes and red lasers it may not be a good idea to allow the solution to swirl with a magnetic stir bar. This is because the free radical oxygen that is released as the dye breaks down into reactive oxygen may be dispersed too quickly. Maybe occainal stirring is optional.

SHOE0007 (author)2016-08-05

Here another graph (Time in seconds) with a pink laser at 200 mw. Assuming 200 mj a second energy. The total power goes to 72 watts optical power for 6 minutes of exposure to the methylene blue dye.

SHOE0007 (author)2016-08-05

The time is seconds and the power is watts.

SHOE0007 (author)2016-08-05

Here is a graph of a red laser beam from Arm laser that can generate quick pulses using a metal fan up near 57 milia joules 660 nm a second. So for 6 minutes you can get over 20 watts of optical power. Please see graph below.

SHOE0007 (author)2016-08-05

I ment to say I could not get a laser with a dye to work at 50 mw 532 mw just scattered light (Intense light).

SHOE0007 (author)2016-08-04

Here is some attempts to make a dye laser with a 532 nm green beam. It did work since the efficiency was less than 1%. It does however create intense yellow light (orange - yellow light) which could be used to create light that could work with MB.

SHOE0007 (author)2016-08-01

200 mw Red laser module.

50 mw Green laser pointer.

75 + mw Violet laser pointer.

About This Instructable




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