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I suggest to use Helicon Focus software. Windows and Mac versions are available at d-Stidio's site
The program is designed for macrophotography, microphotography and hyperfocal landscape photography to cope with the shallow depth-of-field problem.
Helicon Focus also aligns images as objects often change their size and position from shot to shot. This function is especially important for macrophotography.
The program is designed for macrophotography, microphotography and hyperfocal landscape photography to cope with the shallow depth-of-field problem.
Helicon Focus also aligns images as objects often change their size and position from shot to shot. This function is especially important for macrophotography.
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Signing UpStep 1Creating stack of images
You are supposed to work with an optical microscope and a digital camera, or with additional macrolens on digital camera.
- Set your digital camera to manual focusing mode(!!) and set the focus to infinity.
- Manual mode (shutter speed and exposure) is also preferable to avoid fluctuation of brightness.
- Adjust the microscope to make the topmost area of the object sharp.
- Take a shot. Use the remote control (if available) to minimize any shaking of the camera.
- Using the fine adjustment controls, the shift sharp area a little down.
- Take a shot.
- Use small, roughly regular steps while adjusting the mircoscope and taking shots. It is better when sharp areas overlap.
- Take shots until you reach the lowest area of the scene.
- Copy images from the camera to your computer.
- Set your digital camera to manual focusing mode(!!) and set the focus to infinity.
- Manual mode (shutter speed and exposure) is also preferable to avoid fluctuation of brightness.
- Adjust the microscope to make the topmost area of the object sharp.
- Take a shot. Use the remote control (if available) to minimize any shaking of the camera.
- Using the fine adjustment controls, the shift sharp area a little down.
- Take a shot.
- Use small, roughly regular steps while adjusting the mircoscope and taking shots. It is better when sharp areas overlap.
- Take shots until you reach the lowest area of the scene.
- Copy images from the camera to your computer.
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http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artdec02/ahcombinez.html