Step 1: Materials
- Human cells,from the individual who will be cloned.
- Media in which these human cells will grow and divide.
- Media in which cells will stop dividing, and enter a state
of "quiescence" without dying.(freezer)
- Laboratory supplies: Incubator, Sterile Hood, petri dishes,
microscopes, and tools capable of removing and implanting
cellular organelles,from one cell to another.
- Unfertilized human egg cells.
- Media in which the fertilized eggs can grow and divide.
Step 2: Commence cloning!
The second step isn't hard either: Transfer the cells to the freezer. This should allow the cells to live, but they should stop dividing and enter quiescence.
The third step is a hard one for the unexperienced cloner: When the cultured cells are in the quiescent state, get an unfertilized human egg cell. Remove the nucleus from this egg cell. Try to minimize damage done to this cell and discard the nucleus, because this has become useless now.
The forth step is a rather difficult one too:
Take one of the quiescent cells in it's entirely, and implant it inside the coat around the egg (known as the zona pellucida, if you really want to know) next to the egg itself.
The fifth step isn't as hard as step 3 or 4: Electroshock the egg. The electroshock induces the fusion of the two cells, so you should be able to tell when you've electroshocked enough just by looking at the cells.
The sixth (and last)step: Repeat the last three steps as necessary until you have enough clones. Expect a lot of them not to survive because of cellular damage and other mishaps. Allow the embryos to grow and divide a few times in Human Egg Cell growth media. Now implant the embryos in human mothers where they can be carried to term, and born normally. After a few months (approximately 9) you have your own child :)
Step 3: Legal stuff
*All of the European Union
*the UN is against cloning, but doesn't force their members to
So it is not allowed to make a real clone (which renders this Instructable useless XD).
When you really want a child, I suggest you find yourself a wife/man and do it the traditional way (which I personally find to be more fun:P)
This instructable is made for entertainment only, please don't try this at home.