Step 2: Surface Clean and Autoclave Your Glass Petri Dishes

I like to wrap my petri dishes (with lid) in aluminum foil, and also to pre-sterilize my Erlenmeyers just for storage purposes.  This covering allows you to maintain a sterile environment within the wrapping when you take it out of the pressure cooker, but won't prevent the steam from entering and sterilizing your equipment.  REMEMBER, you NEVER want to autoclave a container with a screw top lid on it.  Remove the screw top lid from your slant tube and wrap it separately in foil.  The mouths of Erlenmeyers and the slant tube can be covered in foil.  Autoclave these safely as per previous instruction (pressure can be 'quick-released' to speed up the process, ONLY if you are using 100% borosillicate glass materials), but be patient.  This is a preparation stage and can be done well in advance of actually making solid culture medium plates, if desired.  
Nice work! Thank You.
Yay, science!
<p>I got the idea of testing garlic as antimicrobial- antifungal by reviewing articles. Garlic spears made from sodium alginate and calcium chloride MAY allow for the garlic with buffers to react with the garlic citrate buffer with beads.</p><p><a href="http://engineering.oregonstate.edu/momentum/k12/feb05/M!_GelBeads_final021405.pdf" rel="nofollow">http://engineering.oregonstate.edu/momentum/k12/fe...</a></p><p>I don't know but if stuff like food colouring could be added then garlic in ethanol with citrate could be added. Thus it slowly releases garlic and ethanol into the gelled medium preventing mold from growing!!!</p><p>Any help may be required just Reply here would be greatly appericated. </p>
<p>Another issue is stablity so I strongly recommend adding a buffer like citrate buffer at a pH of around 7.</p>
<p>Atleast 1/4 garlic solution or the oyster mushrooms will not grow.</p>
<p>I am a bit unclear but I mean the solution is made for 100 ml and the garlic would have to be diluted by 1/4 before it enter the agar!!</p>
<p>You now reading this tutorial on yeast gave me an idea. You know GARLIC, I wonder if garlic when combined with sodium dichloroisocyanurate salt solution would be able to allow larger types of fungi like oyster mushrooms to grow without getting hit with pathogens, It would have be pealed garlic cloves right into the agar to prevent mold from growing. It would be a real good idea if this worked.</p><p>Here is a recipe that I made</p><p>10 ml of 0.1% quats. Optional</p><p>0.5% of sodium dichlorocyanate.</p><p>Dextrose (Glucose) 5%.</p><p>Potato starch 5%.</p><p>Optional Soya beans 5g. 5% soya beans.</p><p>15 g agar or 15% agar</p><p>15 g of garlic cloves placed into agar once it starts to boil.</p><p>In my lab I noticed that lots of mold grow in my plates so this is an idea.</p><p>Daniel.</p>
<p>I am for a while tried to grow baker yeast on Nutrient agar which I bought from BOREAL SCIENCE but I just bought maltose from Prolab scientific and it is very expensive 15 dollars for 100 g. So Maltose is required to grow yeast. Would this work with baker yeast and other fungi?? This is because nutrient agar for a strange reason did not grow any yeast expect for pencillium italium instead. The spores in the air were naturally there in small amounts. </p>
<p>This is a pretty awesome tutorial. I am going to try this, but a little differently. I think it would be better to use disposable petri dishes. You can get a 10-pack for like $5 and they are already sterile. Also, it's better to wrap your dishes with parafilm. They will get contaminated if you leave them unsealed in a bag. Plus, you want some gas exchange. After isolation on the petri you can then move to a culture slant.</p>
1000 @°°@ it's me baby !!! Very interesting tutorial. Cheers!!

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