Step 2: Surface clean and autoclave your glass petri dishes

I like to wrap my petri dishes (with lid) in aluminum foil, and also to pre-sterilize my Erlenmeyers just for storage purposes.  This covering allows you to maintain a sterile environment within the wrapping when you take it out of the pressure cooker, but won't prevent the steam from entering and sterilizing your equipment.  REMEMBER, you NEVER want to autoclave a container with a screw top lid on it.  Remove the screw top lid from your slant tube and wrap it separately in foil.  The mouths of Erlenmeyers and the slant tube can be covered in foil.  Autoclave these safely as per previous instruction (pressure can be 'quick-released' to speed up the process, ONLY if you are using 100% borosillicate glass materials), but be patient.  This is a preparation stage and can be done well in advance of actually making solid culture medium plates, if desired.  
Nice work! Thank You.
Yay, science!
<p>I am for a while tried to grow baker yeast on Nutrient agar which I bought from BOREAL SCIENCE but I just bought maltose from Prolab scientific and it is very expensive 15 dollars for 100 g. So Maltose is required to grow yeast. Would this work with baker yeast and other fungi?? This is because nutrient agar for a strange reason did not grow any yeast expect for pencillium italium instead. The spores in the air were naturally there in small amounts. </p>
<p>This is a pretty awesome tutorial. I am going to try this, but a little differently. I think it would be better to use disposable petri dishes. You can get a 10-pack for like $5 and they are already sterile. Also, it's better to wrap your dishes with parafilm. They will get contaminated if you leave them unsealed in a bag. Plus, you want some gas exchange. After isolation on the petri you can then move to a culture slant.</p>
1000 @°°@ it's me baby !!! Very interesting tutorial. Cheers!!

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