Introduction: Lego Microtome

Picture of Lego Microtome
A what?
A microtome is a machine which cuts (usu.) biological specimens into very thin sections. The sections are typically mounted on microscope-slides.

This was an idea I had, I did it, and I've demonstrated it with garlic
It creates slices 250 microns thick*

*calculated



If anyone is actually thinking of building one of these see the last step. You can also PM me if you need more

L

Step 1: Construction: Base and Mount Runners

Picture of Construction: Base and Mount Runners

The specimen sits on a platform which is cranked forwards by gears. The mounting plate is driven by a rack and pinion mechanism geared very low from a hand crank, such that it moves .25mm with each turn of the handle. The platform rests on the red smooth-faced strips, which were lubricated with Johnson's Baby Oil (paraffin).

These images show the base of the device and the red runners for the specimen platform, also the final drive to the rack.

Step 2: Construction: Mount Plate

Picture of Construction: Mount Plate

The plate upon which the specimen is mounted is essentially two smooth runners (lubricated as the previous step), and a rack.
Being Lego, the plate can be easily customised to hold a variety of samples.

Step 3: Construction: Upwards Towards the Gear-train

Picture of Construction: Upwards Towards the Gear-train

Not much to see in this step, I tried to get LDD to work but it hangs ever time (without fail). Perhaps using red instead of black would have been better?
In this sequence you see the front rails that hold the blade assembly being added (yellow and grey)

Step 4: Construction: Gear-train

Picture of Construction: Gear-train

Most of this is low-gearing from the hand-crank.

Step 5: The Blade

Picture of The Blade

I hacked a disposable Bic safety razor for a blade (note that once these blades are out of the plastic, the word "safety" no longer applies - I cut myself twice through carelessness.)

Step 6: Construction: Finishing

Picture of Construction: Finishing

In this step (for the purposes of publishing) I got tired of tedious image-editing, and I'll probably never feel like improving the look of these...
All the way to the end:

Step 7: Additional

Picture of Additional

With the rack-drive movement can be a little "lumpy", which is why I oiled it. A screw-drive would be better, or something with a worm-gear in it.
The gear-train to the blade cranks is a bit too long: all the gears make it a bit loose. It would work better with lever connections.
The blade assembly (grey) needed glue - it picked-off afterwards, but it's not strong enough without.
As you can see from the video it does work, but I needed to be a bit careful.

Your main considerations are:
A good solid platform that is fairly tight
Similarly for the blade
A lot of reduction from the crank. I work out 3 x 5:1 giving 125:1

Disposable razors tend to be held together with little plastic rivets. You can find these as round blobs on the underside - cut these off with a sharp knife and carefully prise the thing apart with the knife. These blades are very sharp and you'll tend to see blood before you feel anything.
To glue it on - I put the blade and Lego on a sheet of cling-film stretched over a tile, and carefully dribbled 2-part epoxy down the edge.

Best wishes

Comments

polarpal99 (author)2017-09-16

Looks like a great way to avoid damage fingers often encountered while using a hand microtome. Do you have a parts list assembled someplace? (Need a shopping list for the Lego store! )

dianadianadiana (author)2015-12-19

So, I'm about to attempt this as a gift to my histotechnology prof.. Wish me luck!

Punkguyta (author)2010-04-06

 Impressive lemonie! Good work

lemonie (author)Punkguyta2010-04-06

I should get the Lego out again, and probably will.

L

Punkguyta (author)lemonie2010-04-06

 Ever since I was a kid I have been dying to get my hands on one of those lego mindstorm robotics kits (I've played/programmed with them before, they're a hoot!), but alas never have as they originally cost over $350!!!!

Do you have any good ideas for implementing something similar with regular lego? Like how would I go about making a robot/remote control car out of plain legos? I have a giant blue tub of lego from when I was a kid, and I'm sure super-glued together would make one hell of a sturdy frame if designed right?

What kind of software do you use to design lego builds? I know there is software that basically lets you build something on the computer first, before you try it, so you can make sure it will work.

ForgerOfPie (author)Punkguyta2011-09-02

If you want something a bit cheaper, maybe you should try something like Arduino. It's not Lego-manufactured, but I've seen plenty of Arduinos users that have hacked lego Technic motors to control them. Mail me if you want resources.

And come on, this is INSTRUCTABLES.COM! I know you can find SOMETHING on here.

lemonie (author)Punkguyta2010-04-06

Go find the lego website (old link step 3) and they'll let you download Lego Digital Designer for free. I'm the sort of person that builds stuff, rather than designs though.
Yea, gluing a frame would work, I think what you want maybe that liquid-glue for making models with (kit military & such). The RC bit, I'd just pull from something else.

L

Cheezpaper (author)2011-05-20

Put a bug in there. Watch the yellow goo gush out.

lemonie (author)Cheezpaper2011-05-21


Cruelty to insects is not one of my favorite pastimes. Still, I appreciate that you had an alternative thought about this.

L

michaelgohjs (author)2010-02-27

cool... btw can u please tell me the gear ratio u used between the blade crank and the shaft to move the garlic (i m familiar with lego parts) 

lemonie (author)michaelgohjs2010-03-06

Step 7, 125:1

L

michaelgohjs (author)lemonie2010-03-24

thx alot .. its gona take alot alot of gears and worm gears 

flashcactus (author)2010-01-19

superb!
I WANT ONE!!!

dissonum (author)2009-08-23

Excellent! As a histotech, I was shocked (SHOCKED!) to see the word "microtome" on the website. Very awesome.

astrong0 (author)2009-08-15

dude you could add a motor to it and make it automated (to a degree)!!

lemonie (author)astrong02009-08-15

Yes. comment Jul 21: I considered the motor drive, but preferred the control of one's hand. L

El Mano (author)2009-08-02

That's awesome!

lemonie (author)El Mano2009-08-02

(the pictures were the worst part...) L

scoochmaroo (author)2009-07-21

Looks like it could easily be automated? I mean if I were using it to cut garlic and not samples. I have way more garlic around.

lemonie (author)scoochmaroo2009-07-21

I thought about a garlic press / mincer, but you know what they can be like to clean. I considered the motor drive, but preferred the control of one's hand. L

scoochmaroo (author)lemonie2009-07-21

totally! I finally invested in a mandolin. I'm going to throw out those chopper things. They look so promising on tv.

fusion (author)scoochmaroo2009-08-01

i wanted to get a mandolin as well, times being what they are and realizing that i only needed it for very thin things, i got the titan peeler with the slicing attachment. works great. plus the julienne. it was a good substitute for the 50$ truffle slicer i was going to get. i'm probably going to do this for dehydrating truffles tho.

yes but didn't you love his nuts?

:)

Itll dry your sweaters............wine coffee cola.......

Rotten194 (author)scoochmaroo2009-07-22

Slap Chop! You're gonna slap all your troubles away with the Slap Chop!

swimmer95 (author)2009-07-29

its to hard to see the pictures!!!! and i want to build it!!

lemonie (author)swimmer952009-07-29

Yes, I wanted to use LDD but it wouldn't work. Had trouble getting the contrasts with a camera, but couldn't improve on this. L

swimmer95 (author)lemonie2009-07-30

well i got about half way and then i couldnt finish because i did something wrong early

swimmer95 (author)2009-07-29

how do u get the left rail on!!!!! help me!!!

lemonie (author)swimmer952009-07-29

Step 3: I had two images the wrong way around, sorry about that. The 5th shows the grey piece with connectors that form part of the front rail guide for the blade (the other side is almost identical). Image 6 shows the rest of this assembly (yellow & grey) and image 7 shows the same piece (complete) for the other side - that one is easy to fit a a complete unit. If this isn't what you're asking about, let me know which step. L

ReCreate (author)2009-07-24

Wow, 200 Microns O_o

lemonie (author)ReCreate2009-07-25

Didn't I say 250? L

ReCreate (author)lemonie2009-07-25

about 200 Microns...

Dr.Paj (author)2009-07-21

I've made one of these before. I only used a spool from sewing string, and a eyelet with a long screw (for hanging things such as swings etc.) and just screw it into the circular opening of the spool. You then would place the object you are cutting (carrot, union, human finger, etc) inside the spools hole. So when u twist the eyelet clockwise it slowly moves the object you are cutting forward. Then cut what protrudes from the end of the spool with a razor blade. This way is way less cool, and its a little more dangerous because you have to handle the blade by hand, but its much faster, and it doesn't require Legos.

lemonie (author)Dr.Paj2009-07-21

I think I see that idea, what were you slicing? L

Dr.Paj (author)lemonie2009-07-24

Anything that fits in the spool that is easily cut by the razor blade. Mainly vegetables so you could look at the cell structure under the microscope.

lemonie (author)Dr.Paj2009-07-24

Hmm, I almost bought a cheap microscope, cheap and not finding a web-cam to go with it were the only things stopping me...

L

luvit (author)2009-07-22

i was looking for instructions for a guillotine...

Fashim (author)luvit2009-07-23

Why?

luvit (author)Fashim2009-07-23

to cut cigars?

Fashim (author)luvit2009-07-23

Ok

lemonie (author)luvit2009-07-22

That's different, but probably easier to do (model not full-size) L

maxxxem (author)2009-07-23

Nerf stephan cutter woot!!! hook up the gears up to NXT i am so building this

isacco (author)2009-07-23

Very good instructable! I rated it 4. Congratulations!

Cat on my Lap (author)2009-07-22

Get an immunohistochemist to give you a tissue sample to cut. If it does so nicely, then you'd really be in the market!

Jayefuu (author)2009-07-22

Very cool lemonie. I like :D

Arbitror (author)2009-07-21

Not bad for Lego!

Jayefuu (author)Arbitror2009-07-22

Lego >> Knex. Lego is way cooler.

lemonie (author)Arbitror2009-07-21

Yeah, try doing this with KNEX? L

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Bio: I'm an experimentalist, a scientist and I have a tendency to do things just for the sake of doing them, or to find out ... More »
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