I was not pleased with the price for Promega and Millipore magnetic separation stands for protein purification ($45 to $377), so I set out to design my own.  In this Instructable I will cover the process of making this simple $3 magnetic separation stand using a 3D printer (PLA) and cheap rare earth magnets, which works just as well as the commercial versions but which costs a hell of a lot less. If you are enterprising, you could even convince your PI that a 3D-printer for your lab makes business sense, given the high cost of many small plastic lab trinkets.  Print off two 8-position magnetic separation stands and you've more than paid for a RepRap! 

Step 1: Print Off the Newest File From Thingiverse

My latest revision of the stand is here: http://www.thingiverse.com/thing:90644

I made it at Techshop (www.techshop.com) on their Replicator 2 (3D printer, in PLA with 3 shells and 35% fill).  If you are curious about my design process, here is my first revision: http://www.thingiverse.com/thing:78964 Since then I have gone through different designs, and even an 8-position version!  Ultimately, the design included an angled center wall, in order to bring the magnets closer to the sidewall of the 1.5 mL microfuge tube.  This increased the speed with which the rare earth magnets can separate the magnetic beads for all your magnetics-based purification needs.  

You can make Taq in your lab -- one of the PIs in my building has never purchased it, and makes enough Pfu pol once a year. Ever purified an enzyme or protein? It's as easy as that.
the plastic equipment is not the expensive part, neither is the machines and computers its the gosh darn reagents that are the expensive part. <br>if you can figure out how to make some taq polymerase at tech shop then we might have something. ;-) <br>you should make a pickpen next. i dont see how a retractable pen with a magnet should cost $500

About This Instructable




More by NonDisjunction:How to make a burner stand for homebrewing out of recycled stools How to make a Quick Key 'Scan Stand' for iPhone 4/4S/5 How to make solid culture medium plates for yeast culture 
Add instructable to: