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A microwave transformer is a thought, but only generates a maximum of about 4.1kV, so you have to be very precise to make a ladder from one of those, but it can be done, I have before. They also draw a lot more power than you really need for this, but again, in a pinch they can work. Be sure to keep the capacitor and diode or you will lose half your output voltage. Replacement mixer diodes can be purchased from many appliance shops, and is often the cause of loss-of-power.
The neon-sign trnasformer is the ideal though, and try to get one from a demolition company (some will save things like this on occasion). Getting one that was discarded may be a crapshoot at best, because usually the primary winding is what burns out.
Best way to find the smallest spark gap is to make just a spark gap starting out too wide (start at 2 inches/5cm), and see at what point it will initiate and maintain a spark. If it will maintain a spark as you try to blow it out, you have a good starting measurement. Do not make the gap to small or you can prematurely burn the transformer out.
Never use wood or wood-products as a separator between electrodes as it is not a good dielectric, and can cause fire. Drill a couple holes in a short length of PVC pipe and bolt it down instead. Your local hardware store will surely have bolts and nuts that match for nearly nothing. Fasteners are the least of the challenge...