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Pearl Gel Box Kit

Pearl Gel Box Kit
 A quick set of instructions to put together your Pearl Gel Box -- comments appreciated!

The instructions are in 4 parts. Page 1 covers soldering steps for the electronics, Page 2 covers installing the electrode wires, Page 3 covers the comb, and Page 4 the gel casting tray.
 
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Step 1Electronics

Electronics
 Tools:
Soldering iron + solder
Wire stripper (optional, if you're good with a pocket knife or scissors)
Small wrench
Loctite threadlocker (optional, helps hold washers onto bolts)

Parts needed from the Pearl Gel Box kit:
Orange gel box lid
2 test leads (1 red, 1 black)
2 Banana jacks (1 red, 1 black), plus washers

1. The first step is to attach the banana jacks to the orange lid. The long threaded part of the banana jack will point away from the lid (Picture 1).
2. Slide the same color cap over the other end (Picture 1, black banana jack)
3. Screw the washer on, add loctite to the joint, and tighten with the small wrench (Picture 2)

Now you're going to solder the test lead onto the banana jack.

4. Strip about 1/2" off of the end of both test leads.
5. Slide the banana jack cap onto both test leads BEFORE you solder them on! (Picture 3). 
6. Make sure the cap is facing the right direction, the cap is going to screw onto the banana jack after you're done soldering.
7. Pick one test lead and twist the copper wire around the unthreaded part of the banana jack so it's able to hold itself on even without soldering. *Keep in mind, this cap is going to need to fit over the joint after you solder.* (Picture 4)
8. Solder the copper wire to the unthreaded part of the banana jack. (Picture 5)
9. Screw the cap on over the joint. Since the cap has plastic threads, be somewhat gentle to avoid stripping the cap. If it doesn't seem to fit on, consider adjusting your soldered joint.
10. Repeat steps 7-9 with the second test lead.
You're all set!
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8 comments
Apr 24, 2010. 1:27 PMlemonie says:
While I have a pretty good idea what this is, other people may not. Could you add into the introduction what you use this for?

L
Apr 24, 2010. 3:36 PMRadBear says:
If I'm not mistaken it is used to run polymerase chain reaction (PCR) experiments. Since DNA has a charge on it you can seperate out various portions of DNA by applying a charge and drawing them through a gel substrate. The smaller the bit of DNA are the farther they travel in the gel.
Apr 25, 2010. 1:24 AMlemonie says:
You'd use it after PCR, the separation is mass/BP/size-based

L
Apr 24, 2010. 8:30 PMmycroftxxx says:
 Isn't this a Gel Electrophoresis kit?  I don't think this has the parts to do PCR.
Apr 25, 2010. 1:21 AMlemonie says:
It sure looks like gel electrophoresis to me.

L
Apr 26, 2010. 5:10 PMRadBear says:
Yep. Gel eletrophoresis. I was close in a not really kind of way. :)
Apr 24, 2010. 2:33 PMAndyGadget says:
Go on - Give us a clue.
Apr 24, 2010. 2:35 PMAndyGadget says:
Found it!  My guess would have been some sort of electro-chromatography, so I wasn't too far off.

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