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Preparing your own thin layer chromatography plates (and then using them)

Step 5Coat glass slides with suspension

Coat glass slides with suspension
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Draw up the newly formed suspension into the syringe.

With the slides cleaned and dried (and free of fingerprints or oils), move the tip of the syringe back and forth width-wise across the slide while applying gentle pressure to the plunger. The motion is sort of like tiling a field. You don't want to pour or dispense it all at once as the layer will form a hill instead, and will be too thick. By going back and forth slowly you can dispense a reasonably even, thin, layer of suspension across the plate.

The thickness of the layer is important, less than 1mm when dry is preferable so be careful not to overdo it.
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