Make a Microbial Fuel Cell (MFC) - Part 1

Make a Microbial Fuel Cell (MFC) - Part 1
In response to numerous questions about what happens to the collected algae this instructable should help someone to build a microbial fuel cell (MFC) with household items and materials. As its name suggests, an MFC uses microbes to catalyze electricity-producing reactions.

This instructable is based on work done by Bruce Logan and his team at Penn State University and on the microbial fuel cells built by Abbie Groff, a student at Conestoga Valley High School in Lancaster, PA. The research she performed with her MFCs helped her win the Grand Champion Award at the 2005 Lancaster County Science Fair.

Now to be completely honest the fuel cell we will build is not "purely" an algae fuel cell, it is a microbial fuel cell that uses anaerobic bacteria to decompose organic matter, in our case dead algae.

The fuel cell will consume the algae (or other organic material) with two significant by-products, electricity (always useful) and methane gas. In a production system the methane must be captured for further use for instance as fuel for a steam powered generator which processes its exhaust plume through an algae based (or other) exhaust gas scrubber.

In this design its very cleverly captured in the sealed anode and if you come up with some clever way to do something with it, I'd be very pleased to hear it.
 
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Step 1Bill of Materials

Bill of Materials
Tools:

Drill or drill press
Razor knife or scissors
Hot glue gun
Funnel - Optional for filling bottles
Soldering iron
Lead Solder
Materials and where I got them. I have no relationship with any of these stores or products. They are inexpensive and should be commonly available:

  • Two heavy duty plastic bottles with sealable lids. Wide mouth bottles are best choice for ease of inserting broad surface area electrodes.
  • Low power aquarium air pump
I recommend the Hagen Elite 799 available at PetSmart for around $6. Rated at 1.8W.
  • 1 x 2" PVC pipe 1-2 feet long
  • 2 x 1 quart mason jars or similar
  • 1 x 2" PVC Nipple or Sprinkler system riser
  • 4 x PVC slip caps. You can probably get away with two, I use two for storing the salt bridge
  • 2 x 1in square Silkscreen material or other nonconductive mesh
Cut to 2 circles that match the outdiameter of the PVC nipple
  • Agar - 100g per Liter of water
  • Salt - 400g per Liter of water ( 14 oz/Qt ).
  • Carbon cloth or carbon paper
Carbon cloth of various types can be purchased online. These folks http://www.uscomposites.com/carbonpage.html offer it for around $40/yd. I'm using plain old fashioned carbon paper with wire mesh support. Eventually I plan to experiment with alternative designs that use carbon filtration technologies.

  • Nitrogen for removing oxygen from water. This can be readily obtained from several sources locally and online.
    • Welding supply places will have Nitrogen and Argon in tanks for various sizes. Around $35 for a small tank here.
    • Local tire dealers. Local tire dealers often replace air in tires with nitrogen (for a fee). They will happily fill up your inner tube or inexpensive pressure tank (for a fee).
    • Bacteria for a MFC can be obtained from several sources.
    • Shultz Liquid Plant food
Most likely, wastewater or anaerobic sediments will initially contain enough organic matter to serve as food for the bacteria, but this will eventually run out. Algae will provide the food source (substrate) used to maintain the MFC. The liquid plant food and soil water will provide trace minerals and nutriets.
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32 comments
Dec 6, 2011. 3:41 PMknexfreak32 says:
Ok thanks.
Dec 4, 2011. 6:04 AMmehulpatel552 says:
hello egbertfitzwilly......
I m making a microbial fuel cell in which i am using a sulfides as a substrate.. and aerobic bacterial cultures. i made a two nylone made chambers with salt bridge. Now i am about to start my work and my guide told me to staderdize a fuel cell,,, so i am not getting how to standerdize a fuel cell with a use of sulfides as a subsrate. i need a help with every type of actions which carry out a good work. it will be a great help of you if you guide me as any......
Dec 3, 2011. 4:41 PMknexfreak32 says:
Do you have to use agar.
Aug 22, 2011. 3:46 AMnshankar says:
Hi,
I'm a student who tried to build an mfc at home. I used waste water as the anode substance, and graphite electrodes, in both anode and cathode. But my multimeter is showing a negative voltage reading. What have I done wrong?
Jul 22, 2011. 2:15 PMrwcrocker says:
A couple of thoughts on de-gassing:

One quick way to degas a solution is to boil it for a few minutes. Gas solubility decreases a lot with temperature. Of course you won't be able to boil your culture, but sterilizing the media might help to keep stray yeasts out of it.
Other gases will work as well, such as helium, which is more readily available for party ballons in smaller disposable cylinders.

You can also remove the oxygen specifically by electrolyzing the solution since you have nice electrodes and a very fine salt bridge. During the electrolysis of salt water, at the cathode (-) side dissolved oxygen is reduced to hydroxide, OH-, and hydrogen gas is evolved. In the other compartment, the anode(+), oxygen gas is evolved, while acidifying the adjacent solution.
Apr 16, 2011. 3:01 AMs208007450 says:
This is really cool. Just wondering how many amps are you able to pull out of it and in that condition what was the output voltage. In other words what's the maximun output power you're able to achieve?

Does the algae have to have sunlight in order to do its thing?

Keep rocking this kind of cool stuff.
Sep 26, 2010. 5:14 PMBoowiggins says:
Idea on methane collection: Add a valved aquarium tube ended in an interface with attachable valved end connected to a lightweight "bag". Put on empty - remove when full, vacuum empty, replace. Make 2 for efficiency.
Aug 21, 2009. 6:08 PMmikedu says:
side topic: New application for MFCs - desalination. This might interest you:

“Water desalination can be accomplished without electrical energy input or high water pressure by using a source of organic matter as the fuel to desalinate water,” reported in a recent online issue of Environmental Science and Technology. Please read more at

http://earthalternate.blogspot.com/2009/08/electricity-and-desalination-from.html
Sep 24, 2010. 12:38 PMdragoninja98 says:
the blog isnt showing up !!!
Sep 24, 2010. 12:37 PMdragoninja98 says:
tell me more
i was looking for this kind of solution for months
wow is this a miracle or what ?
Jul 3, 2010. 11:21 PMsabrina_sita says:
a species of bacteria known as Methanobacterium palustre can convert CO2 to methane. will it work in this setup??
Jul 3, 2010. 10:59 PMsabrina_sita says:
I want to make an electrolytic cell which uses carbon and produces methane as explained in this article: http://www.azonano.com/news.asp?NewsID=17238 The problem is that I don't know how to proceed with this idea. Some Help Please!
Feb 16, 2010. 11:13 AMSionnach94 says:
 I'm a little confused as to exactly what "silkscreen material" means. If its a nonconducive mesh, how will the electrons produced by the anaerobic bacteria get to the cathode?
Feb 16, 2010. 9:49 PMSionnach94 says:
 Are there some alternatives I could use to replace silkscreen material?

I'm not sure where exactly to go to get access to it. Also, should the agar be touching this material when its actually placed over the pipe?

Sorry for taking your time.
Apr 27, 2009. 5:48 PMMr. Green says:
what kind of voltage is it capable of putting out?
May 4, 2009. 9:27 AMmonil singhal says:
How has the methane been captured in the anode? In certain cases even carbon dioxide will be given out as a byproduct in the anode. How can these gases be obtained as an an output from the cell?
Apr 6, 2009. 11:54 PMlemonie says:
What's your connection with Bruce & Abigail?
For the amount of text I'd expect pictures of this being made, can you get some?

L
Apr 7, 2009. 4:50 PMlemonie says:
It'd be good if you could get some more out of them, maybe? L

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