This Instructable tutorial describes how to make a UVB (310nm) transilluminator with a 7 x 7 cm window for viewing ethidium bromide (or SYBR-Safe) stained DNA mini-gels. Once all of the materials are collected, the actual assembly time is approx. 1-2 hours. Some soldering is required.
- Because ethidium bromide is a toxic chemical with strict safety protocols, it is only recommended that you use this dye in a lab with established handling, storage and waste disposal procedures in place. Other users are strongly recommended to use SYBR-Safe instead, which can be handled and disposed of more safely.
- The transilluminator does come with a safety lid for viewing the gel. However, when the lid is not in place, safety glasses must be worn when operating the UVB bulb.
- If you prefer to avoid UVB altogether, we can recommend the blue light LED transilluminators such as the one described in this instructable instead.
Step 1: Materials: Laser cut acrylic parts
Tap holes in the following parts:
- 5-40: Two holes in the enclosure side with the cutout for the power switch
- 8-32: Four holes in the solacryl cover
- 8-32: Four holes in the 0.25" clear lid side part for mounting the hinges
- 8-32: Two holes in the enclosure bottom