Introduction: Will It Centrifuge? (part 2 - Blood!)

Yes, blood!

Only a slide-show again, because I was just taking an opportunity that came up.

A colleague at school did a lamb's-heart dissection, so I grabbed a few millilitres of the blood and centrifuged it at 8,500g for five minutes.

I did not get the results I expected (see the slideshow). I was blaming it on the blood being at least two days old before I got to it, and not kept cool enough in that time, but I have since found out that blood is usually centrifuged for at least half an hour.

Hey-ho.

Comments

author
ClairC1 (author)2015-10-14

how about if i centrifugate a mixture of mammalian blood and distilled water for studying osmosis? thanks a lot

author
Kiteman (author)ClairC12015-10-14

You can try - have a read of the other comments on this instructable, they're from people that actually know what they're talking about.

author
huntermj (author)2008-05-14

Hi, I think you'll find that you're spinning the blood too fast. What happens is the blood cells haemolize and break down leaving you with a lot of liquid tainted with red heme molecules. Next time go 3500rpm for 10 min, thats what we do at the lab where I work.

author
Kiteman (author)huntermj2008-05-14

Oh-ho!

Thank you!

That's one of the things I love about this site - somewhere there's an expert on everything.

I'm part-way through a "how to centrifuge" ible - could you PM me any useful links? Or advice?

author
ChrysN (author)Kiteman2009-06-26

Cool, I never noticed this instructable before. In our lab, we use 1200rpm for 10min. However we need to be gentle with our cells, we want them alive, since we culture them. At this rpm, you will see a layer of red cells, white cells, and serum. Occasionally, we even see an fat layer floating on top. If the blood is old, you'll likely see a clot, sodium heparin in the collection tubes prevent clotting.

author
Kaljakaaleppi (author)ChrysN2013-09-25

Any rough handling breaks some cells. If I recall correctly when you centrifuge cells without an anticoagulant you can't centrifuge the buffy coat, or white Blood cells, visible, they are mixed in the clot. So you either need a test-tube with an anticoagulant (heparin, EDTA or something) or need to add it when drawing the blood. My experiences are from a commercial blood lab where kits are used when possible so amounts of anticoagulant is a mystery to me, it just said 'vacutainer EDTA' on the tubes :).
I tried to look for a method to separate the parts but my source did not tell (qiagen DNA extraction kit manual). Anyway, the rpm doesn't say how much it is in g's, so be careful when spinning.

author
Kiteman (author)ChrysN2009-06-27

Cool, thanks for that info.

author
ejoe (author)2008-11-27

Got a question. I design scientific and medical instruments. In your opinion how do you think blood in urine would settle out in your expected results? Would it mix with the plasma at the top or would it separate from the plasma? I know I should test these things out, just looking for your thoughts. Thanks ejoe

author
Kiteman (author)ejoe2008-11-28

My thoughts are that the cells and platelets would settle out, but the plasma and urine would remain in a fairly homogeneous mixture above. I also think that, if you're designing a test for blood in urine samples, the existing dip-strips are probably more sensitive, quicker and less of a biohazard.

author
Dr. Steel (author)2008-08-28

Awww... now I want some BLOOD! *licks lips in a very hungry (or thirsty in this case) way*

author
ItsTheHobbs (author)2008-05-13

what is a centrifuge?

author
Kiteman (author)ItsTheHobbs2008-05-13

They spin samples really fast to make their component parts settle out. Here, I was attempting to make all the cells in the blood settle to the bottom and leave relatively-clear plasma on the top.

You know how you can keep the water in a bucket when you spin it over your head?

You're putting a force of just over 1 gravity on the water.

Now imagine a machine that spins sample 13,000 times a minute, that's a centrifuge.

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ItsTheHobbs (author)Kiteman2008-05-14

Now that sounds fun

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Cartuner55 (author)ItsTheHobbs2008-07-05

and nauseating

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GorillazMiko (author)2008-05-18

Now we go in search for part 1. +5/5 stars.

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technodude92 (author)2008-05-17

These sideshows have inspired me to make a centrifuge, because its a lot more fun to make one than to buy one. if only i had some proper machine tools.

author
xACIDITYx (author)2008-05-14

Hey, Kiteman. Where could one get a centrifuge for pretty cheap? I saw one online that was 159.99 and it looked pretty decent, but I wouldn't know what one would need to do some tests like this; like g-force wise or size wise. I think the ones I would want would be Small Medical ones. But then again, I have no clue what I need. Then there's the obvious question of whether or not someone could DIY one. Let me know what you think.

author
Kiteman (author)xACIDITYx2008-05-14

There are links to buy a centrifuge like this for around 80GBP in the comments to Part 1, or you could wait for Killerjackalope to finish his how to make a centrifuge 'ible.

author
w00ty32 (author)2008-05-14

i think you watch the will it blendseries a bit too much.

author
killerjackalope (author)2008-05-13

Kiteman, do peanut butter, oh actually it would be interesting to try a few different butters and margarines, see which really has the least oily stuff in it...

author
Kiteman (author)killerjackalope2008-05-13

I'm taking the centrifuge back to school tomorrow, to play with Science Club, so that's a possibility. I'm also part-way through a centrifuging 'ible. I've contacted a couple of the major players in the field for advice, but I haven't had a reply yet.

author
killerjackalope (author)Kiteman2008-05-13

Well food holds some interesting prospects, I'll have to get to work on my centrifuge, at the moment it's a well planned idea, mainly because I don't have a powerful enough motor to hand, though I have a few ideas, including a truly monstrous one, think two foot diameter, around 3500rpm which would be somewhere in the region of 9100G's and it would be capable of taking larger containers... Can you guess what the project is based around yet?

author

I got 4000 g's using your specs.

author

Lol My mistake, working it out in my head, not best advised.

author

Same here (Killer, it's radius in the calculation, not diamter).

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killerjackalope (author)Kiteman2008-05-14

Ooops, a little bad maths, i forgot the exact equation while working that out in me head...

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thematthatter (author)2008-05-13

mr kiteman the best source for blood is inside you! grab a partner, or if your talented you can do it yourself. get a catheter/needle hub (nothing smaller than 20GA)(used for IV) some alcohol pads and a constrictor band and gloves, sharps container and gauze place constrictor band around arm palpate for good vein take alcohol pad and swab around injection site circular, from center out remove catheter from package, remove cap hold the needle bevel up (the slant part is the bevel) with the flash inbetween the finger and thumb of dominant hand. pull skin tight with other hand. insert the needle into the vein at a 20° and stop when the flash fills with blood. (flash is plastic piece on end of catheter ) lower needle until a few degrees above skin and push just alittle more. (if the person feels pain you pushed too far and went through the vein wall) hold the flash with dominate hand, advance the catheter from needle with non dominate hand. (the catheter is a plastic sleeve on end of needle) once catheter is up to the skin press the vein down above the catheter with finger. remove the needle with dominate hand and place in sharps container grab your vial and release pressure on the finger. blood should begin to trickle out. fill up your vial(or if you dont put enough pressure blood will be trickling out already) pinch off vein to close again with finger grab some gauze and place over the injection site. with one quick motion slide out the catheter from arm. pull off constrictor band. place a piece of tape over gauze and your done, fresh homemade blood.

author
Kiteman (author)thematthatter2008-05-14

All easily done if you have the equipment and training.

I have neither.

It is also illegal (in the UK) to perform a medical procedure on another person (like taking a blood sample) without the appropriate qualification (or qualified supervision as part of training).

And I really don't fancy the idea of getting it out of my own vein.

author
thematthatter (author)Kiteman2008-05-14

I learned how through the US army basic training. and for some reason medical people are very eager to stick people with needles, weither they need it or not. almost forgot, there is also a large vein that runs along the front of the doggie equivilant of a forearm. but the vein is prone to rolling, if you prefer K9 blood instead

author

Remove band before you remove needle .....I have done both ways and seems to me less bruising with band removed then with on .....Very cool Kite ....

author

it was just an idea on how to draw up a small amount of blood. i never worked with blood collection, just practiced on initiating saline locks and iVs.

author
Tool Using Animal (author)2008-05-13

But how does it taste?

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Gjdj3 (author)Tool Using Animal2008-05-13

AHHHH! Creepy!

author

... ... ...!

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vanpaun (author)2008-05-13

i LOVE Will It blend

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guyfrom7up (author)2008-05-13

first! it was okk....

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