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Hi, Yes, you can try with lower buffer concentration too. Actually, it wold be better for the signal if you have bigger ionic difference.I also had some problems with induced currents and some shielding might be necessary. I don't think it is a good idea to make your own capillary. The ones I used were 20$ I think (I can dig the info). They need to be 50-75 microns so I am not sure how would you make them.Best
Capillary Electrophoresis View Instructable »