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Keep in mind that "I beg your pardon?" is going to differ widely based on location, conversational participants, and situational needs. If you say "I beg your pardon?" in parts of the Southern part of the US, it may sound as if you're a) offended by what has been said and/or b) looking for a fight. An alternative could be "I missed that." "Could you repeat that?" "I'm sorry?" or any number of other phrases.
Moreover, anything you say will also be changed widely by your cadence, tone of voice, pitch, volume, and the like. I'd imagine that "I beg your pardon?" could be seen in the above way anywhere in the US and elsewhere, dependent upon the pitch and volume of your voice. (i.e. changed pitch, plus changed volume, would likely translate to "I'm offended").
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