mice brains...science research HELP!!! Answered
I am really interested in science research and I want to come up with a research project, but I'm having a hard time putting something together. I want to include aspects like... - PCR (polymerase chain reaction) - RFLP (restriction fragment length polymorphism) - ICC (immunocytochemistry) - e-gel/ gel electrophoresis I know I will be working with mice brains whose vagus nerves have been surgically cut or whose leptin intake has been significantly increased. (By the way, the leptin and vagus nerve are totally different experiments) In this case the vagus nerve serves as a connection from the brain to the stomach. Therefore if it has been cut, mice would show a reduction in food anticipation. The leptin, I believe is a hormone that deals with energy regulation (food intake). Mice with leptin intake grow obese because lack of leptin usually tells the body to stop eating so when the mice with too much leptin are introduced to food, they cannot stop eating. In either case, mice brains would be sacrificed for experimentation. (All experiments are done in a research facility) I'm not sure how to incorporate these procedural methods to the mice brains. My objective is to somehow determine the mechanisms within the nervous system, which can hopefully aid in developing methods to ameliorate neurological problems. I am open to any other methods or protocols.