PCR4all

Introduction: PCR4all

About: Scientific interested in DIY science

Polymerase chain reaction (PCR) is a method widely used in molecular biology to rapidly make millions to billions of copies of a specific DNA sample allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail

specific information about PCR

This biochemical reaction needs one specific instrument : Thermal Cycler

Herein are enclosed all the documentation needed to make your own thermal cycler. This is the first operative prototype, I'm working on a new version, nevertheless the current COVID-19 ha been the main motivation to release the project to the Makers' community.

Attached is the manual for this equipment, which includes the instructions for use, to program PCRs

Step 1: BOM-STL Files

Enclosed is the BOM with some tips,

You also can download the STL files from

STL files -Thinkercad

Step 2: Arduino Shield - PCR Code

Unfortunately, Instructables don't allow me to upload the Gerber files, you can download from here

Gerber files for download

Enclosed are the PCR ino programs

Eng: English

Cat: Català (my mother tongue)

Step 3: Assembly -wiring

Attached are the mounting instructions

Step 4: Principles - Challenges - Acknowledgments

Principles

Those are our principles, and if you don't like them... well, we can find others:
(G. Marx master )

- Open platform

- DIY

- Crowdfunding?

- We like Arduino based electronics

- No business, only sustainable project

- Truly wonderful, the mind of a child is (Yoda master)

- “Salid y disfrutad” (Johan Cruyff)

Challenge

The challenge was to build an instrument as economically as possible, but with a control and programming system suitable for novice PCR users. The current shield needs some changes and improvements. We have the challenge of transforming the current design to be able to make a qPCR.

Acknowledgments

- This instrument has been inspired in The Light Bulb PCR Machine project, which was a clever idea from Russell Durrett. Bulb PCR

- Thanks to Jordi Jordi Soley he made the PCRshield and the PCR program

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    3 Comments

    0
    MIchaelKutscher
    MIchaelKutscher

    Question 1 year ago on Step 4

    Hi, I do find this very interresting. However, could You please include a scematic of the shield? Its not a lot of elements on it and it could well be made with a simple experimenation-shield.
    And a remark: The link Bulb.PCR goes forwarded to some chinese shop (don't understand a word of it)
    Never the less: Thank You very much for the design!
    Kind regards - Michael

    1
    IB-as
    IB-as

    Answer 1 year ago

    Hi, many thanks for your review. I corrected the link and included an schematic. Kind regards