Introduction: PCR4all
Polymerase chain reaction (PCR) is a method widely used in molecular biology to rapidly make millions to billions of copies of a specific DNA sample allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail
specific information about PCR
This biochemical reaction needs one specific instrument : Thermal Cycler
Herein are enclosed all the documentation needed to make your own thermal cycler. This is the first operative prototype, I'm working on a new version, nevertheless the current COVID-19 ha been the main motivation to release the project to the Makers' community.
Attached is the manual for this equipment, which includes the instructions for use, to program PCRs
Attachments
Step 1: BOM-STL Files
Step 2: Arduino Shield - PCR Code
Unfortunately, Instructables don't allow me to upload the Gerber files, you can download from here
Enclosed are the PCR ino programs
Eng: English
Cat: Català (my mother tongue)
Attachments
Step 3: Assembly -wiring
Attached are the mounting instructions
Attachments
Step 4: Principles - Challenges - Acknowledgments
Principles
Those are our principles, and if you don't like them... well, we can find others:
(G. Marx master )
- Open platform
- DIY
- Crowdfunding?
- We like Arduino based electronics
- No business, only sustainable project
- Truly wonderful, the mind of a child is (Yoda master)
- “Salid y disfrutad” (Johan Cruyff)
Challenge
The challenge was to build an instrument as economically as possible, but with a control and programming system suitable for novice PCR users. The current shield needs some changes and improvements. We have the challenge of transforming the current design to be able to make a qPCR.
Acknowledgments
- This instrument has been inspired in The Light Bulb PCR Machine project, which was a clever idea from Russell Durrett. Bulb PCR
- Thanks to Jordi Jordi Soley he made the PCRshield and the PCR program