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Can the large amount of sodium acetate decrease the stability of the glow of TCPO? Answered

Sir we had made the TCPO by ourselves and we used the dye DAPN(diaminp phenazine) instead of 9,10-bis(phenyethyl) anthracene and use the solvent ethyl acetate for this and procced as your procedure but we didnt't get the glow for minutes.It lasts only for seconds(i.e. 3-5).Is this due to the use of DAPN rather than the use of anthracene? We had made the DAPN with the mixture of OPDA(orthophenylene diamine) with Distilled water and the addition of the HRP(horse radish periooxidase). We had used the amount as: 15 ml of Ethyl Acetate 1gm of Sodium Acetate 3mg of DAPN 3ml of hydrogen Pero-oxide 800mg of TCPO Sir, we are the student of Nepal studying Biomedical Engineering and we are doing our final year project and we need your help in this, as our project is based in the che miluminescece method for the pregnency detector.Please,please sir help us sending your reply.We will be very thankful towards you if you will be kind to us.Eagerly waiting for your reply.?




10 years ago

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