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How can I scan to roots of plant that inside of the pot? Answered

Dear friends,

I am an academician in Faculty of Agriculture. Roots development of plants is an important parameter in our experiments. So I need to display the root length without remove the plants from the pots. I need to find the differences between cultivars or treatments about root length, efficient root depth and root area. If I can display it similar to example images, so if I can distinguish the roots by photoshop, It will be so useful for me. Is there any sensor, method or such thing that I can scan the roots without the damage the plants by using of arduino ? or any DIY device. Coloring is not important for me, I need just selection of roots.

Best regards.

Discussions

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steveastrouk

2 years ago

ground penetrating radar ?

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steveastroukJeoleX

Answer 2 years ago

Its extremely short penetrating radar, used for finding structures in the soil

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JeoleXsteveastrouk

Answer 2 years ago

is there any link about this radar, sensoror whatelse. How to do it do you have an idea ?

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steveastroukJeoleX

Answer 2 years ago

I'd look at renting one, its very hard to do technically.

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iceng

2 years ago

I would use a specific granular earth medium size and density so that ultrasonic and or X-ray could distinguish the roots that are a different shape and specific density that even a Pi could resolve to your above style of rendering.

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steveastroukiceng

Answer 2 years ago

I think the design of a suitable representative media would be a major project in itself.

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steveastrouk

2 years ago

I wonder if you could inject a current into the stem, and detect the resulting voltages in the soil. It would be a sort of impedance tomography method.

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JeoleXsteveastrouk

Answer 2 years ago

I have also thought to electric conductivity or impedance method, but there are lot of factor such as soil moisture, soil salinity etc. that could affect to impedance. Is it possible to get display with UV sensor or is there any UV sensor that I can find.

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steveastroukJeoleX

Answer 2 years ago

True, but a lot would calibrate out.

How would a UV sensor work ? Your roots are buried !!

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JeoleXsteveastrouk

Answer 2 years ago

hımm what about thermal sensors. maybe I can catch an image and screen roots.

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steveastroukJeoleX

Answer 2 years ago

HOW. Your roots are buried. Soil is not transparent at ANY wavelength. You might have to characterise your soil very carefully, but I think impedance tomography is your best chance.

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JeoleXsteveastrouk

Answer 2 years ago

Assume that inject a current to stem, but under the different irrigation regimes, We couldn't say that yes this impedance value gives to us information about roots dimension. We have to consider all conditions are same for using impedance method. But in my experiment, I will have different environmental conditions such as temperature, soil moisture, air humidity, light intensity and etc. and need to measure root development periodically.

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steveastroukJeoleX

Answer 2 years ago

You'd get an approximation, because you could also independently monitor soil conductivity away from the injection site.

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JeoleXsteveastrouk

Answer 2 years ago

I need to measure also zero point for using this impedance method and take out this value from my first value that root and soil impedance. So it is useful, maybe give the differences of applications, but I have some doubts about be accepted by academic commission in sci journals. However, if I can screen I mean get a photo of root under the soil, I can show my proof and It could be accepted easily.

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steveastroukJeoleX

Answer 2 years ago

You have a whole new method for examining root systems. Mention me on the research papers.....

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Yonatan24

2 years ago

Acrylic container with orbeez (silicone balls)? Would that work?

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JeoleXYonatan24

Answer 2 years ago

Why not, but it is soilless culture. I wanna get regression between field and pot experiment. It is the most accurate measurement that if we can screen the root inside of the soil. I think.

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Downunder35m

2 years ago

The only way I know would be by placing the seedling into a square and clear plastic tube/container without a bottom.
That way you can remove the soil in front of it for visual inspection and once stablished remove the plastic.
Depending on the plant you need different sizes so that the root development is a bit limited to inside the available space.

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JeoleXDownunder35m

Answer 2 years ago

I know this method, but I need to screen the root development dynamically without damage to plant. So I can create a graphic that about roots development day by day.

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Kiteman

2 years ago

Why not grow them in a clear gel? You won't need any special sensors, just a ruler.

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JeoleXKiteman

Answer 2 years ago

We generally carry out field experiments. There are lot of factor that affect to root growing in the soil. So growing inside of soil mixture in the pots would give the more accurate results as similar to field experiment. On the other hand, you can only screen to roots of plants which are young seedling. But if you can scan to roots inside of pots, it will have more wide growth period based on your dimension of pots.