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How to create an Adiabatic, Isochloric and Isobaric Environment? Answered

I am trying to make an chamber to examine the growth of a micro organism in the above condition. The chamber temp. should be maintained at 95-100 C and. The environment should be tends to vacuum(Vacuum condition is practically impossible). Please help in choosing the material and conditioning the same.

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dashinfoline
dashinfoline

9 years ago

Thank you friends for showing your interest and helping me out in making my project.

Please help me more precisely in choosing the chamber material.
It is the main thing which is stooping to proceed ahead.

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sidgupta
sidgupta

Answer 8 years ago

I would suggest using a borosil glass cylinder( they are designed to withstand all kinds of temperatures and pressures ). Moreover they are fully transparent. If transparency is not needed then stainless steel or aluminium is good( because it does not rust). The chamber should have a bottom section filled with water which can be heated( to vary humidity) and the entire thing must be placed in a thermos flask to help maintain the conditions.

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steveastrouk
steveastrouk

9 years ago

Metal. Preferably stainless.

How big does it need to be ?

Steve

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lemonie
lemonie

Answer 9 years ago

A chemist says glass, an engineer says metal...

L

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steveastrouk
steveastrouk

Answer 9 years ago

True.

But surely a chemist who wanted to grow stuff in a giant box would go for metal.....

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lemonie
lemonie

Answer 9 years ago

I know people who grow things in giant boxes... Mind you, that's not at low pressure and 100oC. The reference to examining things suggests this would be experimental rather than production to me.

L

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dashinfoline
dashinfoline

Answer 9 years ago

This is purely meant for observation of micro organisms in extreme conditions

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lemonie
lemonie

Answer 9 years ago

I know, get 'em sealed in ampoules and heat them with a steam-bath.

L

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dashinfoline
dashinfoline

Answer 9 years ago

Steam Bath is the best option to heat the chamber externally in a controlled way.
I am going to use it.

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steveastrouk
steveastrouk

Answer 9 years ago

We made a convector oven around ours - a big circulating fan, and a heater coil.

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lemonie
lemonie

Answer 9 years ago

Unless you're below sea-level or under a high-pressure weather phenomenon you can't top 100oC and you're steady with the atmospheric-pressure at the time.
Last time I measured boiling water it was 98, but I'm getting towards 1,000 ft up.

L

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dashinfoline
dashinfoline

Answer 9 years ago

It is an cylindrical chamber of diameter 20cm and height 20cm.

Vacuum Chamber.png
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iceng
iceng

9 years ago

Nalgene

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dashinfoline
dashinfoline

9 years ago

Please give me some more details on choosing the material of chamber.
I am going to use LM36 as temp. sensor, a pressure sensor and a pH sensor.

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lemonie
lemonie

9 years ago

Get a glass-blower to seal em in ampoules. If you freeze them in liquid nitrogen first and pump out the air you'll get your least gas-environment.
You need a lot of kit for this, obviously.

L