Coke or Pepsi

What pop do you like more coke or pepsi?      Coke      Pepsi          9              1

Topic by luke96 9 years ago  |  last reply 8 years ago


Holiday Spice Pepsi

Hey all - I was wondering if anyone had a recipe or any idea of what spices/flavorings go into Holiday Spice Pepsi.  My boyfriend and I are addicted to it and Pepsi doesn't make it anymore and has no plans to in the future. Any suggestions would be great!

Topic by Moosaka 9 years ago  |  last reply 4 years ago


Pepsi Refresh Submission

Please cast your vote for the CIR's Pepsi Refresh submission to Provide Cost-Effective Prosthetic Care in Underserved Midwestern Areas - http://pep.si/eo57my Video for the submission is available at: http://www.youtube.com/watch?v=x7gVKMypR7E

Topic by CIRnetwork 8 years ago  |  last reply 8 years ago


who thinks pepsi tastes like coke opened after a day?

Who thinks pepsi tastes like coke opened after a day? i think thats what pepsi tastes like . you can just pour day old coke in a pepsi can and call it pepsi. no offence

Topic by mberg 7 years ago  |  last reply 5 years ago


Why participate in Pepsi greenwash?

I don't know about the rest of Instructables community, but I am extremely reluctant to contribute anything to Pepsi's latest (https://www.instructables.com/contest/pepsirefreshproject/)  greenwash campaign, well, anything except criticism. PepsiCo (PEP) is a company that produces semi-addictive, food-like consumables that, in the aggregate, are actually harming people's health. They're also littering the world with PET plastic bottles. Previous Instructables alliances with companies that make tools or building supplies (Black and Decker, Leatherman, Gorilla Glue), these alliances made sense to me since these companies actually make products to help people to build things. PepsiCo is essentially a manufacturer of recreational over-the-counter drugs, similar to beer, liquor, cigarettes.  Thus alliances with entertainment industry (e.g. ball games, car racing, crappy pop musicians) would make sense to me, but not an alliance with Instructables.com, whom I like to think is doing something more noble than just crass, brain-numbing entertainment. If you'd like to see PepsiCo's official explanation of this "Refresh the World" business, but without being tracked by DoubleClick, the direct link is: http://www.refresheverything.com/how-it-works Pepsi says they want to change the world, but I think they just want to sell more soft drinks.  I don't see them as a serious agent for change in the world.  IMHO, the revolution will NOT go better with Pepsi! Update:  Many of people responding to this topic seem to think that I'm lobbying for the prohibition of soft drinks, but this is not my goal. In truth I wish more recreational drugs would be allowed to have the same legal "white market" status that sweetened drinks and snack foods enjoy. The point I was trying to make is that Pepsi is an inappropriate choice for an Instructables alliance because the basic values of the two companies are so different. Pepsi does not embody the DIY (do it yourself) philosophy. Rather the values of PepsiCo are convenience and instant gratification. This is essentially a SEDIFY philosophy (somebody else do it for you), the polar opposite of DIY. The reason I mentioned harm to the heath of people and the planet, is just to demonstrate that Pepsi does have cause to want to improve, or greenwash, their image, which is I suspect the point of their "refresh project", not just pure philanthropy.

Topic by Jack A Lopez 9 years ago  |  last reply 9 years ago


Which is better? Coke? Or Pepsi?

What do you think is better? Coke or Pepsi? I prefer coke.

Topic by ry25920 11 years ago  |  last reply 11 years ago


Wick Pepsi Can Stove

I made a regular can stove recently, that runs off of denatured alcohol. If you don't already know how it works, search wikipedia: "can stove". Anyway, I also took two can bottoms about 3/4" high each, cut two slits in one, and pressed it inside another. Then, I cut a hole in the middle of the top, about 3/8" wide. I then put a few 1" lengths of 1/4" pure cotton rope in that, for a wick. To fill it up, I dripped denatured alcohol in the bowl shape with the wick, and into the cans. Once I felt that there was enough, I stopped. I lit it, and it worked great, but after a while, I saw flame leaks around the seal of the cans, and heard the alcohol boiling. Then I thought, what if I poked holes around the can top, and let that relieve the pressure, while also providing a great flame.I haven't tried this yet, but if I do, I want to know whether to take pictures, and make it into an instructable. Below is the .gif animation I did in Inkscape demonstrating my idea.

Topic by John Smith 11 years ago  |  last reply 10 years ago


The Neighbors Project

Today's feature is the Neighbors Project. How well do you know your neighbors, and your neighborhood? The Neighbors Project covers some easy ways to get acquainted. Start with the basics, like How to say Hi to a stranger on the street and How to make sure your neighbors get their mail if it ends up in your box, then move on to more complicated projects like How to get a tree planted on your block and How to increase produce in your local corner store. Your neighborhood is what you make it, so choose to make your community a better place! The Neighbors Project can help. Check them out, and get inspired. So, what are you doing in your neighborhood?  How would you like to help out? This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 9 years ago


How would i make a can dispenser?

I just bought a stock pile of pepsi cans, 330 ml.  And i'm bored of them just lying on the floor, and also need something to do.... I have masses of lego, and have seen many working, vending machines that dispense cans. Such as; http://www.youtube.com/watch?v=UU1VCtobxkw  I would like to make something very similar to that, And really want to know how to make a simple pepsi can dispenser. Any help appreciated, (:

Question by Squiggmont 9 years ago  |  last reply 8 years ago


Support The Eyewriter

One of my favorite projects on the site, the Eyewriter, is in the running to win $250,000 in the Pepsi Refresh Everything Contest. If they win, they plan to use the money to make and distribute 1,000 units of the device, as well as develop a better user interface. Should they win, it would be a great opportunity to empower paralyzed individuals to express themselves creatively and live more fully. There is about two weeks left to show your support by casting your vote. The project is currently in 40th place and voting ends August 31st. They can use all the support they can get. Let's help get them to #1! You can cast your vote here.

Topic by randofo 8 years ago  |  last reply 8 years ago


Water Recycling with a Hydroponic Bog Garden

Clean water - we all need it.  Treating and reusing sewage and other waste water means reducing the burden on the already limited supply of water from our lakes, rivers, and underground aquifers. Instructables user Biotank posted this excellent Hydroponic bog garden. It's the final step in a series of sewage water treatments, and uses bacteria to clean the water even further so it can be used for irrigation.  This system can be combined with a garden for water-loving plants as shown here, or with a fish pond.  While this bog garden isn't likely to fit in your backyard, it's a great example of thinking outside the box to solve the problem of water reuse!  Check it out, and get inspired. What are you doing to conserve or reuse water?  What would you like to do? This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 9 years ago


cut plastic pepsi bottles? What is the best method?

I am trying to do a project with plastic pop bottles. I have to make three cuts and am doing it with sissors. My hand is killing me!!! I have looked at the foam wire cutters but nowhere does it say that you can cut plastic using them.

Question by 9 years ago  |  last reply 8 years ago


Create awareness of a particular diet choice...

What would make the world a better place...depends how you define world, and your benchmark for what distinguishes better place.  This contest suggests things that would improve home, neighborhood, society, and planet.  I have an idea that would improve all of the above! Health: Personal back story:  Growing up I drank a lot of pop.  It was not uncommon to polish of a 12 pack of soda in a day or two, and this would happen regularly.  For the last fifteen years I've consumed at least 2-4 liters of cola per week, if you average it out (probably closer to ten).  Thats a conservative 2000 litres of cola.  At ~120 grams of sugar per Liter, that works out to a whopping 240 KILOGRAMS of sugar.  Now, were my parents terrible?  I don't think so - the knowledge wasnt out there that us kids were being fed a high sugar highly addictive substance that really messed with our bodies.   I can personally account that I had a terrible sleep schedule growing up, and I can directly attribute it to sugar/caffeine.  Unlike many friends I know I managed to stay skinny and somewhat 'in shape' through hyperactivity.  The artificial insomnia destroyed my school scores - as I spent a lot of time very tired in class, not paying attention - and was even suggested to go on medications to 'fix' how 'weird' I acted.  Most all of my child teeth and adult teeth are full of fillings where I had cavities - and I can't have cold food touch my teeth because I have almost no enamel left.  Consider now the direct economic cost of this:  At an average of a dollar per liter - multiply by the number of 'addicted' heavy user kids out there, and you have yourself a staggering amount.  I'm not alone.  I know lots of friends with similar stories - some less fortunate with serious health problems like diabetes and bariatric problems.  Economy: The high fructose corn syrup industry has halfway destroyed the cane sugar market in many third world countries.  Soda machines all over, often in schools, rape the pocketbooks of young persons for a product they don't need.  Pretend for a moment you are a drug dealer gang boss.  Now imagine you can put a legal salesman in most every school, "free".  I don't need to explain the rest of the story. Society:  Soft drinks are not 'evil' - they are an enjoyable vice that when taken in reasonable quantity aren't that harmful.  Therein lies the problem - they are marketed as the be-all and end-all to be happy, thirst quenched, and popular.  Couple this with the fact that they contain high levels of two of the most addictive legal substances out there:  Caffeine and glucose.  Our nervous systems don't stand a chance. Environment:  Frankly you'd be surprised how much carbon dioxide comes from the soda industry - that fizz goes somewhere after you *kssshk* open the can.  High fructose corn syrup is bad not just for your body but for the environment.  Some areas are very good at recycling, but still others are brutally abysmal at their three R's.  Many COUNTRIES in fact don't recycle at all.  That's a LOT of plastic and aluminum ending up in the environment, to be there long after we are gone. My proposal:  What I want to see is awareness campaigns of the health risks of being a heavy user - in conjunction with warning labels similar to what the tobacco industry has on their products.  I want to see their huge profits going into the community (like in this contest - more of it!).  Make companies accountable for their products. photo courtesy freefoto.com

Topic by frollard 9 years ago  |  last reply 9 years ago


COKE MACHINE OF HAPPINESS!!!!!!

 This video is awesome!!!! Rival Pepsi would never think of this!!!!                      !!!!!!!!!!WATCH AND ENJOY!!!!!!!! !!!!!!!!!!!!!!!!!!!!!AND SUBSCRIBE TO ME!!!!!!!!!!!!!!!!!!

Topic by KnexFreek 9 years ago  |  last reply 8 years ago


My pepsi can stove won't light... Possible solutions?

I used a design with 24 holes-top burner and an inside wall with 3 notches for the denatured alcohol to drain in between walls i used several measures of lighting zippo, match, and a combo of zippo and alcohol all useless. I noticed a small crack on upper half I thought about using JB weld but I thought that I would first ask if I would have to remake the top PS I used Arizona cans PPS My tools are: Exacto knife, Dremel 100 series with starter attachments, scissors, templates from zen stoves,and stuff that is basically a given standard tools hammer, nails, screwdriver,etc.

Question by bwcaw101 9 years ago  |  last reply 8 years ago


My candles are sinking? Answered

I made some candles earlier today, by melting existing candles and pouring them into a mould ( a pepsi can) 2 hours later this has happened (see below)! Why has this happened and how can I reverse/ stop it?

Question by roblin90 9 years ago  |  last reply 9 years ago


Dachshund wheelchair

Dachshunds are adorable little dogs, bred for chasing badgers and other burrowing animals. Unfortunately, their elongated backs are delicate, and prone to all types of injury. Enter joelsprayberry's Dachshund wheelchair. It's an inexpensive way to support the dog's back legs until he can walk again.  Reading through the comments is inspiring - so many people have found this Instructable, and built their dogs wheelchairs.  Instead of having to put the dog down, it can recover and enjoy a full life. There's even a ferret version. Any more heart-warming pet stories to share?   This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 9 years ago


Is it wrong for my health? I know it's wrong but!!! Answered

I just swallowed  abut 3 teaspoons of coffee,drank a glarss of pepsi, about 200ml of 42% alcohol cane liquor at a party. what are the effects? quick buffor i fueint outt

Question by ARJOON 7 years ago  |  last reply 7 years ago


Build an Electric Car

Want to convert your car to all-electric on the cheap?  Check out bennelson's awesome Instructable, Build your own Electric Car!  Ben walks you through the steps of converting a Geo Metro (purchase price $500, gas-requiring parts sold for $550) to run on a forklift motor and 6 12V batteries.   According to his calculations, the car gets the equivalent of about 130 mpg.  Not bad for an entirely self-taught mechanic! Check out the video of his converted Metro going up hills: Sounds easy, right?  So when are you planning to convert your car or truck? This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 7 years ago


Water-saving toilet hack

Toilets use fresh water, and lots of it.  Sure, you can buy a low-flow model, but you're still flushing with a (smaller) quantity of fresh, otherwise drinkable water. Gregorylavoie decided to get double use out of that water by hacking his toilet.  It's a neat, reversible modification: after you flush, you use the clean, incoming tank refill water for handwashing.  Your slightly soapy hand-wash runoff fills the tank, and is saved for the next flush.  It's a simple, ingenious way to save water with little to no effort. What do you think?  Would you try this mod on your toilet? This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 6 years ago


Quilt-in for charity

Want to make beautiful quilts for charity? Host a quilt-in event! Author auntwrenny tells us how the Crafting for Charity Club holds a quilt-in every year, getting a bunch of crafters together to make quilts for groups in need.  This past year, they made an incredible 15 quilts in 12 hours, then distributed the quilts to homeless people in the Des Moines area. See the Instructable for suggestions on publicizing, organizing, and running your own quilt-in!  Auntwrenny covers everything from soliciting donations, setting up different quilt-making stations, great jobs for kids, and the importance of keeping your volunteers well-fed! Inspired?  Want to run your own event?  Have other great ideas for charitable crafting? Be sure to leave a question or comment on the Quilt-In Instructable! This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 5 years ago


Mini heather-stove-combustion chamber for diesel fuel droplet

Hi word! I like to find if there is a project, or  it is made already, a mini  stove, gravity filled diesel fuel, droplet, which burn with blue flame. Not bigger than a alcohol stove made out from pepsi - bier can. Appreciated any answer about this topic. Kresimir Pregernik  dipl.ing.

Topic by KresimirPregernik 5 years ago


Universal Nut Sheller

Today's feature is the awesome Universal Nut Sheller. Designed by Jock Brandis of the Full Belly Project for a women's coop in Mali, this tool dramatically increases peanut-shelling speed from 2 lbs per hour (hand-shelling) to 110 lbs per hour.  Since roughly half a billion people in the world rely on peanuts as a primary protein source, this nut sheller has the potential for serious impact. The machine is simple to make, relatively inexpensive, and can shell pecans, pine nuts, neem nuts, shea netus, Jatropha, and other nuts as well as peanuts.  Molds and metal parts can be ordered from the Full Belly Project. Check out the 2005 video introducing the machine to Uganda: Check it out, and get inspired.  What can you do to help people in developing countries?  We need more ideas, the simpler the better. This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 9 years ago


Plastic Soda Bottle Prosthesis

Prosthethic arms for land-mine victims, etc. War, land mines, and natural disasters create far too many amputees each year, especially in the developing world.  Worse, prosthetic limbs are expensive and hard to come by.  The Center for International Rehabilitation, posting under Instructables username CIRNetwork, has been working to find sustainable solutions:  locally available materials worked by local craftsmen.   Their plastic soda bottle prosthesis is one clear, simple solution to this problem:  a plastic bottle is heat-shrunk to cover a plaster cast of the limb stump.  Different devices can be attached to the end of the bottle, enabling the wearer to perform basic daily tasks.  This type of thing can really make a difference.  Check out the video below, and see more on the CIRNetwork YouTube channel. Want to help?  CIR is seeking donations of mobility aids to support ongoing rehabilitation relief efforts in Haiti.  You can also make general donations to assist people with disabilities worldwide. This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 8 years ago


Is it possible to get gas out of a liquid other than by boiling?

Still putting my solar device together and I'm still having some trouble getting the working fluid (ethanol) to boil. Almost there. I was wondering tho if there's not an easier way. Is it possible to by adding heat, make a gas dissolved in a fluid bubble out. Like if you heat up something like pepsi, get all the CO2 to gasify. And, how would you get the gas back in?

Topic by SolarFlower_org 9 years ago  |  last reply 9 years ago


Build PV solar panels from broken solar cells

The sun's energy may be free, but solar panels can be quite expensive.  Still hoping to get off the grid? VIRON has a great Instructable, How to make PV solar panels, teaching how to make your own from broken or surplus solar cells! From the introduction: In essence, this involves ways to connect cells, which may produce more or less than one volt, and not only try to increase power output but also decrease the load, that is, efficiently conserve the energy whether it is meager or significant. For example, even the weakest solar panels can run watches, calculators, radios, charge batteries, and if a computer were specifically designed to, it would be as solar-powerable as a calculator. Check out the Instructable for info on finding and foraging usable solar cells, instructions on preparing different types of broken cells, tips for getting more power out of your panel, and instructions for wiring the whole thing up to power your devices. Inspired? Planning to try piecing your own solar panels?  Already off the grid, and planning something even better?  If you do, be sure to post an Instructable so the rest of us can learn something new. This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 9 years ago


how can i make a hole in a glass bottle? Answered

Okay so i have this sweet glass Pepsi bottle that i found at the store. And i wanna make a bong with it. I don't have any glass drill bits and don't really wanna buy any either. I did see a video where a kid cut a bottle in half with nail polish remover ( http://www.youtube.com/watch?v=_A4J7RcdsfM&feature;=related )ANYONE KNOW HOW TO DO THIS? ALSO WHAT SHOULD i USE FOR THE BOWL?THANKS :]

Question by beauwalker23 10 years ago  |  last reply 3 years ago


Tim Anderson

Meet Tim Anderson! He's the co-founder of Z-Corp 3D printers, host of the Know How Show, author of the Heirloom Technology column in MAKE magazine, and the most prolific Instructables author with 222 projects and counting! So, besides teaching you how to get a free yacht and how to fly a hydrofoil, what is Tim doing for the betterment of humanity?  Here's how he describes it in his profile: Tim's philosophy involves building minimum-consumption personal infrastructure from recycled scavenged materials.  Redirecting the waste stream.  Doing much with little.  A reverse peace-corps to learn from poor people all over the world. In practice, this means traveling the world documenting traditional Pacific Island sailing canoes, collecting handy tricks from Australia, Guatemala, Indonesia, and the US, and teaching us how to repair or reuse almost anything.  (When you're done with the Handy Tricks series, check out his Instructable showing How Not To.)   You can also learn how to move a tree, or keep an old truck running. He's a believer in Garbage Santa, who leaves "trash" treats for creative people to find and repurpose, and can do almost anything with recycled bike innertubes.  He's also an enthusiastic sailor, bicyclist, and kiter who builds all of his own equipment - check out his ice gear in this video: So, are you inspired yet?  Do you believe in Garbage Santa?  And which Tim Anderson project are you most likely to try? This post has been sponsored by Pepsi. The Pepsi Refresh Project celebrates the people, businesses, and non-profits with ideas that will have a positive effect on our world.

Topic by canida 9 years ago  |  last reply 9 years ago


Which soda is the best?

I suddenly got this idea to post a topic in which soda is the best. If this topic has already been posted, please notify me so that I can remove it. Votes will be closed at October 15. The winning soda will be announced after I count all the votes (either the 15th or 16th), and whoever voted for the winning soda gets the good feeling of being better than everyone else. Capiche? (8.12.08) EDIT: Wow. I never thought this would become so popular in about... 5 days. Thanks for voting, everyone! RULES: 1. You may vote for up to three DIFFERENT sodas, once each time. 2. You may not vote more than once. 3. You may not start a conversation with someone on this forum that has absolutely nothing to do with the topic. 4. There is no #4. 5. Your choices are: Anything you like that's a soda. Remember to keep your votes to up to 3 different sodas. Nothing alcoholic, please. EDIT (8.13.08, somewhere around 12 PM Central Time) The results so far. In first: Coke with 5 votes. In second: Jones with 4 votes. And the sodas tied for third: Root Beer, Cream Soda, and Dr. Pepper, each with 3 votes. Sodas with 2 votes: Mountain Dew, Diet Coke. Pepsi, and Canada Dry. Sodas with 1 vote: SURGE!!!!!!, Virgin Cuba Libre, Sprite, Sprite Remix (-_-;;), Vanilla Coke, Sunkist, Cherry Coke, Fresca, Limca, and 7-Up. Thanks again for voting, everyone!

Topic by RokuXIII 10 years ago  |  last reply 5 years ago


Cleaning House....by house I mean machine shop

Ok...finally compiled a list of everything that's going so I don't have to make multiple posts... this is it:Water Heater 10 gal. Electric 22x24" $100CRT Monitors Qty. 4 FreeToledo Scale Vintage Counting Scale $100Electrical Boxes Various sizes, shapes $10eaDayton Right Angle Motors (Davit motors) qty2 (L&R;) 1/35hp from a boat model 5K5355 RPM 1725 $300 for both or $200eaExtrusion Various sizes, shapes, qty. $1/lbCable winch from boat trailer $20Time Clocks Mechanical, broken $20 for bothBrewmatic Junior Industrial Coffee Pot $60Baseboard Heater $15Matsuura tool holders *NEW* (qty2) setting/changing fixtures fits BT40 $470 for both or $275ea2 Spring Testers 1 torsion $Price unknown at this time1BridgePort DRO +Scales Scales OK, DRO broken $100Mitutoyo Touch Signal Probe 192-001 $115Micrometers Mitutoyo 8-9" part# 103-223 $125 & 10-11" part# 103-225 $135 or $200 for bothGarnet Hopper Jet Edge new this runs $8700! $800Spring Wire Various sizes, stainless (17-7, 302) and music wire $1/lb - large coils (pm/email for list)Drill Sharpener Darex M4 w/ accessories $500Drill Sharpener SRD 80-M $400Garmin GPS Old, still works! $10Briggs & Stratton Motor NEW. 18hp cast iron, twin cyl. 964cc $999Metal Desk 60" wide, seafoam green metal $60Hose Clamps 5.25" in diameter $.25/eaCoffing Hoist 1/2 Ton 3 phase hoist. Works, but is dirty. Has controls $450Pepsi Machines 2 older can machines. They work & we have keys $100Lip Cutter/Grinder Alexander 2CG (for engraving tools)w/accessories $600Capacitance Meter Emco Capacitance Meter, old, good condition. $20All prices are obo. It can be shipped UPS (sometimes USPS). I have pictures for most of the things on the list.Email garagesale@pittraffic.com if you are interested. I don't check PMs very often, but PMs ok too!THANKS!

Topic by Rainbowlaces 9 years ago  |  last reply 9 years ago


You can help save a rare frog from extinction!

First off, I realize instructables does not want pure advertising on their site through these forms. The voting is optional and I don't want this to be viewed as advertisement. If nothing else, I want to share with you our story in the project, and hopefully you'll see how awesome this is. We are doing something that no high school has ever achieved before, and I think it's more relevant to the site than some re-posting of a blog article. I hope that you take a few minutes and find out what the Frog Project is all about! Want to support the cause of helping endangered species, education, and advancing biotech in schools? Well, take 5 minutes and vote for our school to convince Khols' to give us 1/2 million $$! Unfortunately the contest is over, but we're going to go for a $250,000 grant for a Pepsi contest in November. If you become a fan of our page (noted below) you'll be updated. Check back in November! https://www.facebook.com/pages/The-AAEC-Frog-Project/128763967175416 ======================================================== Not convinced? Well, here's our story: You can help save a rare frog from extinction, and it is free! Kohls the dept. store is donating $500,000 to the 20 schools with the most creative need for the money. Your vote can help our school win this money, and best of all it costs you nothing! We are working on a project to try and save a rare frog from extinction. And we need the cash. At our small high school of 300 students, we are trying to completely sequence the genome of a rare Arizona frog. We hope to accomplish 2 things from this work. 1) We hope to rescue this frog from extinction. 2) We hope to train a new generation of students in modern biotechnology, and rescue the State of Arizona from last place in quality of education in the United States. The Chiricahua Leopard frog is currently listed as a threatened species. We have been working in conjunction with the Phoenix Zoo and Arizona Game and Fish to develop a genetic test that could aid in conservation efforts. We have already spent a year working on the sequence of the mitochondrial chromosome of this frog. Our school actually owns a DNA sequencer. We have made pioneering efforts in the design of genetic tests for this frog. Our students have presented 5 posters on our work at the International Plant and Animal Genome conference (PAG), quite an achievement for a high school. Our project was recently awarded one of only three heritage grants: http://www.gf.state.az.us/w_c/heritage_projects.shtml By the way, the other two Heritage grants were awarded to Arizona State University and Northern Arizona University. What we really wish to do is completely sequence every chromosome in this frog. This is a very ambitious project. Consider that the human genome project completed in 2003, cost $3 billion dollars and took 13 years. Our leopard frog is estimated to contain twice as much DNA as humans! However, we think that with appropriate funding, and new technology that has recently become available we can accomplish this task in 2 years! We wish to construct what are called “DNA libraries”, collections of broken chromosome fragments of known sizes. These can be sequenced quickly using NEXT generation sequencing technology. The Chinese genomic group BGI has agreed to cover the costs of NEXT generation sequencing, and genome assembly for our project (an estimated $1 million) if our school can come up with the remaining funds needed for the construction of the libraries. We need to purchase about $200,000 in new equipment, and extend our existing lab space. With the completed genome sequence, we would like to have a custom “gene chip” manufactured. This chip will have all the DNA of the frog physically printed on it. The chip allows experiments to be carried out without any harm to any frog. This chip could then be used to aid conservation efforts by the Phoenix Zoo and Arizona Game & Fish. It could allow the exact parentage of every Chiricahua frog that is discovered to be known. Eventually this technology will become the backbone of conservation work on all species, but at this early stage it is not possible for Arizona Game & Fish to raise the funds needed to generate a gene chip before it is too late for the frog. Our school hopes to be the first in history to carry out a genome project. We hope this will serve as a guiding light to other schools, and start a grass-roots movement in the conservation and education world. Help us save our frog! Here is the catch. Vote for us on Facebook, and convince all your friends to do the same (forward this message to them), or unfortunately the frog bites it! Everybody can vote up to 5 times for us. In this way YOU can help make the difference not only for this rare species, but if we can succeed you will help pave the way to help many others. https://apps.facebook.com/KohlsCares/school/32741/aaec-paradise-valley Regards, -Mike Dr. Mike Brown Science Dept. Chair AAEC-Paradise Valley Phoenix Arizona 602 569 1101 Interesting references/links about our project and research (really, we are real!): Abstracts to posters presented by our high school students at the Plant ANimal Genome Conference (PAG)http://www.intl-pag.org/17/abstracts/P03a_PAGXVII_102.html http://www.intl-pag.org/pag/17/abstracts/P03b_PAGXVII_134.html http://www.intl-pag.org/18/abstracts/P03d_PAGXVIII_162.html http://www.intl-pag.org/18/abstracts/P01_PAGXVIII_031.html http://www.intl-pag.org/18/abstracts/P03b_PAGXVIII_155.html Our high school and its project http://www.aaechighschools.com/teachers/pv/mbrown/abstract.htm The MoBio scientific supply company featured us in their 2009 catalog, and describes our project on their “where in the world” page of their website: http://www.mobio.com/pages/wiw-namerica.html See page 8 of the Phoenix Zoo’s Conservation Science Newsletter: http://www.phoenixzoo.org/learn/Conservation_and_Science_Newsletter_Winter_09.pdf A recent article from the press: http://www.somonews.com/article.cfm?articleID=18154

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