0avocadostains 10 months ago ReplyUpvoteIt looks like it is arduino controlled, which is awesome, but beyond my understanding, so I can't help you there. Is the incubator built yet? What are the temperature/light/humidity requirements of E. coli. Is it something where a simple temperature switch wired to a light bulb would be good enough? https://www.ebay.com/itm/2Pcs-10A-250V-KSD301-30-C... (Such as those) Also there are good DIY Sous Vide instructables that would provide more precise temp control, depending on which controller you buy, you can also control humidity levels. I'm sure you can do all that with Arduino too, but I wouldn't know where to start. Also, I just kind of took a leap of faith and assumed you weren't a bioterrorist or something? Why exactly are you culturing E.coli? I had that before. It was gnarly, bloody diarhhea for 2 weeks or so.