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Know how to make Butanol/BioButanol? Answered

I've been recently reading up on Bio diesel and I've found that butanol is great for cars that run on gasoline/unleaded petrol.

Does anyone know where there is any reliable information on how to make bio-butanol, Maybe out of algae?

Wiki Biobutanol reference


biobutanol costs about $1.60 per gallon to make (this does not include the cost of heating) for the other ingredients and that is if you are making 1000+ gallons up at a time. the real cost is the infrastructure ($10,000+ for a small plant we can make) and highway taxes, about 0.65-0.85 cents per gallon. The feds and state want there money. the university of Arkansas has a diagram on how to make it but they do not give what is called (for us in the design world) "mass flows" and temperatures needed, out. But it is the closest thing for you and I to use. easy to figure out the rest of it. The algae is easy to get. Order from just about anywhere. it takes about 120 cubic feet of water, grow lights, and 60 pounds of CO2 a day to make 40 pounds of algae which will make about 70 ounces of biobutanol. anyone can make algae. anyone can make butanol. just don't drive on the road (all states own the roads, not you and I, and as such the roads come under contract law, every state has it, with the full force of enforcement to the contract to use the roads, hence, a drivers license, car license and,,,, to use the roads) with it in your tank. farm/off road use only. biobutanol also has a "spec" on what it should be for highway motor fuel. they can get you that way to. One of the reasons for the high cost.


4 years ago

SIR/MS: Sierra Club adherents and Biomass Supporters need to solicit our Senators and Congress to alter the new "Biomass Thermal Utilization Act (BTU ACT) to include large tax credits ($1.00 per gallon) for gas stations to sell Butanol Gas Blends (24%), Hydrogen Gas, Bio Deisel, CNG (Propane & Natural Gas) and installation of related kits for both Cars and Trucks. And tax credits for individuals whom get engine conversions to burn Bio Diesels and other alternate energy fuels. Likewise, and local state EPA supported coal to liquids or gas conversion plants should be Federally EPA approved automatically. And the largest Ethanol Plants (production over 20 million gallons per year) should be given Federal Funding to convert to Butanol Production (Cost is $15 Million each). This is needed to make the United States energy self sufficient and to give every American some relief from high Fuel & Energy costs and costs associated with transportation of goods via truck.

Qcks, you can by Clostridum Clostridium acetobutylicum from Carolina Supply company... I like your comment about using cow manure (fresh).. I have tried that and it works, but I was looking for just the strain by themselves.. I saw that in one of my dad's science catalogs and looked that up on the internet...

I thought that you might be interested... Just another idea...

The link is http://www.carolina.com/product/living+organisms/prokaryotes/bacteria+cultures+and+sets/chromobacterium+violaceum%2C+microkwik+culture%26reg-%2C+pathogen%2C+vial.do?keyword=%22clostridium+acetobutylicum%22&sortby=bestMatches&page=1

One living culture is $9.75 per tube...

You can then create subcultures of them to make more of them first or just ust that strain and purchasemore; then put them in your sugar solutions and get butanol, ethanol, and acetone...

thanks.. for your idea... It helped me...

I'm not 100%, but, i'm pretty sure you need to register with some governmental organization in order to order that sorta stuff. I tried to order cyano bacteria cultures (from the Anabaena genius) from texas university my freshman year of college and they wouldn't sell their stuff to me without a permit number.

They didn't mention which regulatory agency i needed to talk to for the permit.

Cultivating wild cultures can have some advantages, but i can understand if you're not interested in doing so. research into 4 carbon fermentation can really stink.



8 years ago

Eh.... I have NO desire to mess with Clostridium species bacteria, as some are extremely dangerous, but they're by no means hard to get ahold of.

Here's how to do it in 5 easy steps.
1. take a mason jar and fill it with hot sugar water.
2. let the sugar water cool to room temperature.
3. find a cow.
4. wait for cow to take a big fresh dump.
5 Quickly put the poo in the water.

You now have a clostridium culture.
Clostridium bacteria are obligate anaerobes so heating the water to reduce oxygen concentration is essential. 
yeast and other aerobic beasties present in the cow's feces should remove any free oxygen in the mix, as well as pulymerizing the sucrose dimer for metabolism by the clostridium.

I sorta have to ask why you're looking for an alternative fuel. Butanol really offers none of the benefits that other ecologically friendly products do, and it has many of their same draw backs.
Just off the top of my head, Methanol can be blended with other carbon bearing compounds to burn in a similair fashion as gasoline, but it has the advantage of being infinitely easier to produce then butanol or ethanol. In fact, Pyrolysis can be used to make methanol, and since Methanol is used in making biodiesel, even if you don't use it directly, someone will, so it won't go to waste.

Thanks for the answer. As I asked this so long ago, I now have no clue as to why I asked this. I shall have to try and remember tat, so the information does not go to waste.

from the looks of it no for now due to the bio enginered yeast till someone discovers something that does the same thing looks like we are out of luck or till someonemanages to liberate some of the yeast (be free little ones) and they multiply ( shock in someones basement/garage <sudders at the thought of theft> ) and leave the nest to other friendly/un friendly garages and basements till then sorry folks but if i find something will post asap

I believe that butanol is harder than ethanol to make as a bio-fuel (that is, a product of fermentation) because it is more toxic than ethanol: it kills the bugs that make it.

There are many web references to butanol biofuel, but they mainly seem to be sourced from the same website: http://www.butanol.com/

Although it seems to have many benefits over ethanol (mainly that it is less explosive, has a higher energy-density and is able to directly replace gasoline without engine modification), it does have the same feedstock as ethanol, which makes it as much of a competitor for food-crops as ethanol is.

Since ethanol is easier to make by many producers (alcohol fermentation is not protected by so many patents as ButyFuel's process, so licensing will not be a problem), I expect butanol will probably end up as the betamax fuel - better at the job, but left behind in the marketing scramble.

Oh, Thanks for the comment (and the link) That will keep me thinking for some time...

I dug through the wiki article and the butanol site, though it didn't explain the advantages it did explain that Butalco use modified yeast to make butanol.

Yeasts as production organisms for biobutanol have decisive advantages compared to bacteria: Yeasts have GRAS (Generally Recognized as Safe) status and are easy to genetically modify. They are well established, highly robust, and the production process is easier to control. As ethanol producers are used to working with yeast, it is much more probable that the ethanol production plants in Brazil, Europe or North America will use yeasts rather than bacteria should they switch from ethanol to butanol. - That's Butalcos line on it.

Though they don't purify, they only ferment... which seems like an odd thing to do. They do explain another reason in almost the same spiel on a different page, they say that yeast is what ethanol manufacturers are used to so the switch from ethanol to butanol would be as painless as possible...

They've apparently got funding from people, which could be good news but it's from Volkswind who are an independent Power Producer of wind energy (bad translation maybe be a factor in that sentence) so far the new says it's all research but they're applying for patents and such. They go out of their way to avoid using the words genetically modify, unless they have a way of tricking yeast in to making a new product another way, granted they use the same process to make loads of stuff, insulin for one.

Lignocellulose is the planned one, it says it works with c5/c6 sugars...

My thoughts would be that the yeast would work the same with a different output, however the raised toxicity still gets called in to question, if they ever considered it carefully enough and engineered them to make a non water soluble byprodcut the yeast wouldn't die, I think, the yeast mightn't work properly anymore so another bacteria base but basically self removing from water, maybe a gas would be easier, I'm thinking in terms of conversion efficiency, sure butanol is a good hydrocarbon for energy density but is it more efficient to make ethanol or maybe methane, or methanol, a gas would take the least work to separate and a bit extra to store, though it's prefectly pipeable, how efficient are process that make larger hydrocarbons from gases like methane? is it more effective to do it that way round? because distillation is energy intensive plus you still don't get that much of anything from your original brew, with plenty of feedstock and decent conditions couldn't breaking down wood or any organic waste in a way similar to composting be effective at making methane, kind of like the people that make electric from "biofuel reactors" - Basically what I outlined except the feedstock is pig **** and the breakdown is natural, then it's a generator burning methane.

<rant closed>

Re-opened... Just for thermo's sake... I'd quite like to be powered by a waste silo, how funny it would be to turn round and say, yep, my computer, house and car, powered by pig ****

Yeah that would be pretty cool So do you think it is possible to make butanol/bio butanol at home?

I have no idea.