If these speakers are amplified, yes. If not, no.Do they have batteries or an ac plug? If yes, it's amplified.To do it, attach a mono 1/4" adapter onto the speaker's stereo 3.5mm jack. Plug this into your guitar.Problem is, it probably won't be very loud. These speakers are usually designed to take a headphone-level input and not a totally un-amplified signal. Still, if your speakers do have power, they will likely get it loud enough to at least hear. Best results if you can plug headphones into one of the speakers and listen through those.Want a listenable signal? Find anything, a tape deck, another powered speaker, a mixer, etc that also amplifies a little bit and feed it through that first. I use an old RCA mixer which amplifies just enough for me to record. I've also used a cheap toy megaphone, with the mic removed and replaced with a jack, as a practice amp... I only mention it to hopefully boost your creativity a little and help you solve your problem.