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mice brains...science research HELP!!! Answered

I am really interested in science research and I want to come up with a research project, but I'm having a hard time putting something together. I want to include aspects like... - PCR (polymerase chain reaction) - RFLP (restriction fragment length polymorphism) - ICC (immunocytochemistry) - e-gel/ gel electrophoresis I know I will be working with mice brains whose vagus nerves have been surgically cut or whose leptin intake has been significantly increased. (By the way, the leptin and vagus nerve are totally different experiments) In this case the vagus nerve serves as a connection from the brain to the stomach. Therefore if it has been cut, mice would show a reduction in food anticipation. The leptin, I believe is a hormone that deals with energy regulation (food intake). Mice with leptin intake grow obese because lack of leptin usually tells the body to stop eating so when the mice with too much leptin are introduced to food, they cannot stop eating. In either case, mice brains would be sacrificed for experimentation. (All experiments are done in a research facility) I'm not sure how to incorporate these procedural methods to the mice brains. My objective is to somehow determine the mechanisms within the nervous system, which can hopefully aid in developing methods to ameliorate neurological problems. I am open to any other methods or protocols.


The only thing I understood was gel electrophoresis! You lost me.

well...I just want to format a research plan using molecular methods to test mice brains for some kind of indication of arousal. My lab wants to use food associated hormones like leptin or parts of the body like the vagus nerve. I'm just having a hard time connecting gel electrophoresis and the other protocols to get the results I want. PCR is just amplifying DNA, which would probably be used for gel electrophoresis or e-gel. ICC is immunocytochemistry where you take antibodies and tag a certain cell (in my case we locate neurons in the brain). The result gives you a visible look at activated cells within the tissue you're testing. For example, I used c-Fos (which is activated when the cell is activated) and used c-Fos antibody to attach to c-Fos in the cells. In short, the antibodies used are tagged with substances that enable activated cells to appear under a light microscope or fluorescent microscope.

I understand now (I already knew polymerase chain reaction and the nervous system stuff) Sounds cool. Can't wait to see some results

Whoa! That sounds insane!

Jeez, you're dealing with some pretty technical stuff, It'd be interesting to see if you could wire the mouse's brain up with some electrodes and somehow stimulate it to do things - sort of like remote control. Also, it seems as if hormones have quite a large effect on the mouses behavior, and I'm guessing that the hormones are stimulating the brain, so perhaps tubes to the brain through which hormones can be uh... distributed. I'm really going out on a limb here, though - considering I'm suggesting attempting mind control and I don't have a good background in biology.

However Christy (Canida) has her SB in Biology from MIT, and I'm sure that she might have some insight!

haha thanx we already worked with using electrodes to stimulate the brain, but we found that it's not really accurate and pretty uncomfortable for the mice.

Dude! Seriously?! That's sooo cool!

well, keep on truckin' on - you're bound to find some help around here!

Try PMing Christy for some advice, perhaps, if you haven't already.

Yeah, I had ICC as the Interstate Commerce Commission. c4thy, you will really help yourself if you will spell out your acronyms - for the rest of your life. Not every time, but at least once in anything you write down. Unless you are writing a note to your partner, parent, or someone who know exactly what you're talking about.

haha sorry
I wasn't really in the most "awake-mode" when I was writing this...but if it helps?...
icc= immunocytochemistry