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  • Jaswant61 commented on jbumstead's instructable Desktop CT and 3D Scanner With Arduino5 weeks ago
    Desktop CT and 3D Scanner With Arduino

    There is a published protocol called ClearSee for whole plant tissues (leaves, seedlings, mature stems, pistils, moss) (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712841/#DEV127613C20). In short, ClearSee is a solution of 10% xylitol powder (w/v), 15% sodium deoxycholate and 25% urea in water and fixed tissues are stored in this solution for 4 days (leaves) to 4 weeks (mature stems).An older method of using acidified chloral hydrate has also been improved recently (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105042/) that uses 45 g chloral hydrate dissolved in 25 mL 4.2% HCl to which 10 mL glycerol is added.

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  • Jaswant61 commented on jbumstead's instructable Desktop CT and 3D Scanner With Arduino5 weeks ago
    Desktop CT and 3D Scanner With Arduino

    Fantastic project. I may build one soon.There are now lot of techniques to "clarify" or make objects transparent, particularly animal tissues (e.g. mouse brain, small larvae). Initial boiling and placing in saturated sugar solution may work for some fruits /vegtables (you may have to store for months!). One of the old methods for animal tissues used to use benzyl benzoate. More recent techniques use some form of urea or acrylamide (caution, this is neurotoxic/carcinogenic chemical) .e.g. Clarity (https://en.wikipedia.org/wiki/CLARITY). There is lot of development in this area due to a new technique called light-sheet microscopy.I teach and research at the University of Saskatchewan and do experiments at the Canadian Light Source (that uses synchrotron x-rays) using phase-contr...

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    Fantastic project. I may build one soon.There are now lot of techniques to "clarify" or make objects transparent, particularly animal tissues (e.g. mouse brain, small larvae). Initial boiling and placing in saturated sugar solution may work for some fruits /vegtables (you may have to store for months!). One of the old methods for animal tissues used to use benzyl benzoate. More recent techniques use some form of urea or acrylamide (caution, this is neurotoxic/carcinogenic chemical) .e.g. Clarity (https://en.wikipedia.org/wiki/CLARITY). There is lot of development in this area due to a new technique called light-sheet microscopy.I teach and research at the University of Saskatchewan and do experiments at the Canadian Light Source (that uses synchrotron x-rays) using phase-contrast CT. We gather projection images and do the processing using shareware softwares to generate 3D stacks of images. One of the easiest to use is Bruker Instruments NRecon (32-bit or 64-bit freeware version) that can be downloaded from http://bruker-microct.com/products/downloads.htm. Jaswant

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