In previous Instructables tutorials we have described how to make equipment used for DNA electrophoresis and imaging. These include a mini-gel electrophoresis tank
, a UV transilluminator
for ethidium bromide gels and a blue LED transilluminator
for sybr-safe gels.
An additional piece of hardware required for electrophoresis & gel imaging is the electrophoresis power supply
. This high voltage power supply connects to an electrophoresis tank setting up an electric field between the two electrodes. DNA samples loaded into an agarose gel move through the gel towards the anode (+ve) with the agarose gel matrix separating the DNA molecules by size (see Step 5 for an example).
Electrophoresis power supplies typically have a variable output voltage allowing the user to set the output voltage for different size gel tanks and modify voltage for optimum results and convenience.
In this Instructable we describe how to make a variable electrophoresis power supply suitable for mini-gels. The design is based on the Maxim high efficiency MAX1771 step-up DC-DC controller and boosts a 15V input, from an external wall-wart power supply, to an adjustable 25-100V output. The power supply uses a switch-mode design in a step-up (boost) topology - as shown in figure 2 of Maxim's "An Introduction to switch-mode power supplies".
An important source of inspiration for this design was the open source 150-220V Nixie tube power supply
designed by Nick de Smith.
A schematic of the power supply design is shown in the images above. The fundamental components of the design are the MAX1771 DC-DC controller (U1), a MOSFET switch (T1), an inductor (L1), a diode (D1) and an output capacitor (C5). In the design the controller regulates the output voltage via a Pulse-Frequency Modulated
(PFM) signal applied to the gate of the MOSFET. When this signal is high the MOSFET switch is turned on and when this signal is low the MOSFET is turned off. The controller adjusts the pulse rate of the PFM signal, based on feedback from the output, in order to maintain a constant output voltage. The PFM signal divides the operating cycle of the power supply into charging and discharging phases. During the charging phase, the MOSFET is turned on and energy is stored in the inductor, the diode is reverse biased preventing current flow, and the load is supported by energy stored in the output capacitor. During the discharging phase the MOSFET is turned off, the diode is forward biased and energy is transferred from the inductor to the load and the output capacitor.
The magnitude of output voltage of the power supply is set using a voltage divider
on the feedback from the output to the DC-DC controller. In the schematic this voltage divider consists of resistors R1, R3 and RV1. Using the values of these resistors the output voltage of the supply can be determined via the formula Vout = Vref (R2/(R3 + RV1) + 1) where Vref has a value of 1.5 V. For this design we used fixed resistors for R2 and R3 with values of 1M ohm and 15k ohm respectively. For RV1 we selected a variable resistor with 0-50k ohm range. Plugging these values into the formula above gives a theoretical output range of roughly 25-100 V.
When determining the required voltage output, we followed the recommended guidelines of 5 V/cm, where cm refers to the distance between the two electrodes. For our mini-gel system
, electrode distance is 17 cm, so ideally we should run the gel at 85 V. Open source hardware
- This is an open source hardware project licensed under the Creative Commons Attribution 3.0 License
. The design files can be found on Bitbucket here https://bitbucket.org/iorodeo/hv_switching_psu
and here https://bitbucket.org/iorodeo/hv_switching_psu_enclosure
This Instructable is written in collaboration with willrodeo