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My interest in Mycology and Tissue culture started long back which led me to collect few laboratory equipment like Petri dishes, test tubes, weighing equipment, measuring glasses and also chemicals like Hydrogen Peroxide, Agar-agar and Potassium Permanganate. One equipment I wanted most but beyond my reach was a Laminar Flow Hood.

A Laminar Flow Hood provides a small sterile work area, where you can carry out all your culture works without the risk of contamination, simply by flowing completely purified air at a specified rate. However, the cost of the simplest form of the equipment is very prohibitive... the one with the very lowest specifications costs more than one thousand US Dollars... large corporations, universities, and research institutions can afford this but individual  hobbyists like me can not.

The alternative...? Construct your own equipment...

There are two main types of Laminar Flow Hoods : Horizontal Airflow and Vertical Airflow. In a horizontal type, the purified air is blown from the back and moves towards the front of the working area. In a vertical type, the air is blown from the top over the working area and moves out through the base

I have carried out extensive study and came out with a design for constructing a Horizontal type Laminar Flow Hood, making use of a vacuum cleaner for the blower and press board / Plexiglas for the hood body thus reducing the cost considerably.

This Instructable will provide you step-by-step instructions on how to build your own portable Laminar Flow Hood without any compromise on standard specifications.
 
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JeremyB153 hours ago

I have been contemplating using a vacuum cleaner for a while, I have been assembling parts and I want to use one very badly for simplicity and cost. Everywhere, I read to use a blower from 400-600 CFM which seems like an overkill. Your vacuum is 42 CFM (20 LPS). My question is simply, does it work? Have you been contaminate free?

Awesome build man! You broke some rules, your a guy of my own heart. :-)

antoniraj (author)  JeremyB152 hours ago

Why not...? For the hobbyist who need only a small sterile space to carry out his experiments, this is sufficient. The laminar flow hood should provide a clean work area with purified air blowing at the rate of 0.5 mtrs per second which we are getting through the vacuum cleaner. More over the air passing through the vacuum cleaner is pre-filtered and almost clean. Please see Step 1 where I made some calculations. If you already have a vacuum cleaner, restrict the hepa filter area matching with the air flow of your vacuum cleaner. 400-600 CFM airflow is for larger hoods used in laboratories.

Lastly, thank you for your appreciation...

smxles7 days ago

this is incredible! can you email the instructions via email at smxles@gmail.com

antoniraj (author)  smxles6 days ago
please go through attached files sent to your mail
MuhammadF3012 days ago

Its a great invention! Can you please email me the instruction via email at m.fikri@outlook.my

antoniraj (author)  MuhammadF3011 days ago
Please see attached files sent to your mail
EristalU2 months ago

Thank you for sharing! Will you please share your instructions via email with me as well? 2013.utest@gmail.com

Thank you!

antoniraj (author)  EristalU1 month ago

please see files sent to your mail

DevinF21 month ago

Aloha,

Would you be able to send me the copy of instructions as well? My email is devin.frederciicksen@gmail.com. Thank you so much

antoniraj (author)  DevinF21 month ago
Please see attached files sent to your mail
Thank you for your response, however, I misspelled my email. It is devin.fredericksen@gmail.com. Would you mind sending the instructions again? Thank you
antoniraj (author)  DevinF21 month ago

ok, I have resent the files

such a great invetion sir, could you sent me the copy of instruction? these my email iqbalskeleton@gmail.com Thank you

antoniraj (author)  skeletoniqbal1 month ago
please see attached file sent to your mail

Hi Antoniraj,

I interested in this. Could you please email instructions to jsxventure@comcast.net

Thanks you!

antoniraj (author)  JimmyTHEWISEGUY2 months ago
Please see attached files sent to your mail
homopue2 months ago

Hi antoniraj,

very interested in your work.

Could you send me the instructions as well?!

Thank you!

homopue[at]gmail[dot]com

antoniraj (author)  homopue2 months ago
Please see attached files sent to your mail
evanjamesc2 months ago

Could you send me the instructions? evanarchiejames@gmail.com

antoniraj (author)  evanjamesc2 months ago
please see attached files sent to your mail
DerrickT32 months ago
cristofero3 months ago
Hi antoniraj..could you please send me plans for the flowhood? Did you have success with growing mycillium using the unit you made?
antoniraj (author)  cristofero3 months ago
please give me your mail id, I will send the construction details to you. I have not tried it as I got busy with other projects but you can use this for growing mycillium
TobeySynBio3 months ago

Hi AntoniRaj, I am also very interested. Can you please send me the instructions to tobyworldwide[at]web[dot]de ? I would appreciate that a lot! Thank you, Toby

antoniraj (author)  TobeySynBio3 months ago
Please refer to attached files sent to your mail
ahenrique43 months ago

Hi Antoniraj, I enjoyed so much your project, could you please send me the instruction for my adress? andrehenrique_bio@yahoo.com.br

Thank you

antoniraj (author)  ahenrique43 months ago
Hi ahenrique, please see the attached file sent to your mail

Thank you so much.

Hi AntoniRaj, I'm very interested in this project, could you please send me the instruction to this address? bewsenzio@gmail.com

antoniraj (author)  lorenzo.bewsenzio5 months ago

Please see the attached files sent to your mail for more information

Hello antoniraj, Can you please send me the instruction for a vertical type hood to my email fluna@upgto.edu.mx.

Thank you very much for your help. I think the world needs more people as you because you give real help.

antoniraj (author)  francisco.luna.9461795 months ago

thank you... Please see attached file sent to your mail for construction details of vertical type Laminar Flow Hood

tomn45 months ago

Sorry but this is a total waste of time and money. For laminar flow you need the proper hepa filter matched with the proper blower to achieve 100 cfm. Anything else wont proved true lamiar flow. Also pressurized glove boxes defeat the purpose and increase contaminants. The way a glove box works is to create dead air with no turbulence so the continents drop to the bottom after a spray with water.

antoniraj (author)  tomn45 months ago
I don't think you have gone through all the steps of this instructable fully.
ErnestM15 months ago

Very great read. love this article. would like to know more details. please send it at zerisvapes@gmail.com. TIA!

antoniraj (author)  ErnestM15 months ago
Please go through attached files sent to your mail for more information
thebobr.7 months ago

ok...so i'm preparing to build mine but i'm thinking if i could make few changes. For instance.

UV lighting. You can get cheap UV LED they should work just as good.

I'm thinking of making the whole body out of plexi glass and making it a bit smaller as first of all i have not a lot of space on my desk, second of all plexi glass is expensive in my local building supplies depo.

Now i could just use a vacuum cleaner, i may even have one somewhere in the attic but if to try push the cost down even more :) Do you think that this is going to work if I use different filter configuration...for example I already have HEPA filter that is going to be installed just like in your hood, but if I fix and additional paper filter on the entry pipe this should do the job shouldn't it?

What worries me the most is the actual vacuum cleaner, it seems...loud, not economical as it will consume a lot of energyand it will take a lot of spaces. I've been thinking if different motor can be used and if so what power would be suitable...For instance i've got this little electric air pump (used for kayak, mattrace etc) the airflow on it is 680 l/minute. it is 5 times smaller than a vaccum it can be made much more quite and it can work on batteries....

antoniraj (author)  thebobr.7 months ago

you can make any changes you want to the basic design. The aim is to get a sterile work area.

Regarding UV lighting there are many options available in the market. You can install UV LED lighting also, but make sure it is UVC.

To cut cost, you can use plexi glass in front and at top for better visibility.

If you have a HEPA filter available with you then a paper filter at the inlet will also do the job. But the problem is cleaning. In my design all particles are collected by the vacuum cleaner filter & it requires cleaning and replacing that filter only. The vacuum cleaner will also do the job of cleaning the HEPA filter by reverse flow of air.

Regarding the smaller blower instead of the vacuum cleaner, please carry out a little calculation with reference to the area of HEPA filter surface as I have shown in Step 1 of this instructable. If it is within limits you can use it.

hadrien.velge7 months ago

Could you send the instructions to hadrien.velge@gmail.com ? Thank you very much!!

antoniraj (author)  hadrien.velge7 months ago
Please see files sent to your mail
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