Instructables
Picture of Portable Laminar Flow Hood
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My interest in Mycology and Tissue culture started long back which led me to collect few laboratory equipment like Petri dishes, test tubes, weighing equipment, measuring glasses and also chemicals like Hydrogen Peroxide, Agar-agar and Potassium Permanganate. One equipment I wanted most but beyond my reach was a Laminar Flow Hood.

A Laminar Flow Hood provides a small sterile work area, where you can carry out all your culture works without the risk of contamination, simply by flowing completely purified air at a specified rate. However, the cost of the simplest form of the equipment is very prohibitive... the one with the very lowest specifications costs more than one thousand US Dollars... large corporations, universities, and research institutions can afford this but individual  hobbyists like me can not.

The alternative...? Construct your own equipment...

There are two main types of Laminar Flow Hoods : Horizontal Airflow and Vertical Airflow. In a horizontal type, the purified air is blown from the back and moves towards the front of the working area. In a vertical type, the air is blown from the top over the working area and moves out through the base

I have carried out extensive study and came out with a design for constructing a Horizontal type Laminar Flow Hood, making use of a vacuum cleaner for the blower and press board / Plexiglas for the hood body thus reducing the cost considerably.

This Instructable will provide you step-by-step instructions on how to build your own portable Laminar Flow Hood without any compromise on standard specifications.
 
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nrajsathya2 months ago

Hello Sir, Can you please send me the instruction for a vertical type hood to my email id. nraj.sathya@gmail.com,

Thank you very much for your help.

antoniraj (author)  nrajsathya2 months ago
please refer to the file sent to your mail id...

Hello there sir! Could you send me more details at mrmghz1@gmail.com? I appreciate this instructable greatly

antoniraj (author)  TylerM25 days ago

please go through files sent to your mail

akumar2049 months ago
Very much useful sir! I need to build one like this for mushroom spawn production sir, send me more details to arunengg85@gmail.com. Thanks in advance
antoniraj (author)  akumar2049 months ago
please go through the basics sent to your mail id
holly-g9 months ago
What's it used for, domestically?
antoniraj (author)  holly-g9 months ago
It is a laboratory equipment used for tissue culture, Mycelium culture in mushroom growing and used by scientists / research persons for other purposes also.. this provides an uncontaminated microorganism free work-space
carlos66ba9 months ago
Very well done! Suggestions: use fluorescent uvc bulb rather than incandescent for higher UV intensity and less heat; add a uvc bulb to the main chamber to sterilize as needed (normally should be off, and add a switch that turns that bulb off automatically if the gate is open). Lastly: is flexiglass sufficiently opaque to UVC?
antoniraj (author)  carlos66ba9 months ago
thank you carlos66ba... regarding your suggestions...

I have searched for a whole day in a big city near me and could not find any UVC bulb being sold by electrical shops.. I could lay my hand on this bulb through ebay India and at that time it was the only one available there. So, there was no choice for me.. I will replace it with fluorescent uvc bulb whenever I can find them. I will add this to the main chamber.

I think adding a pressure operated switch with the front opening will automatically turn off the uvc lighting whenever the gate is opened. it can be very easily done.

Lastly, though there are many suggestions, I don't think plexiglas is sufficiently opaque to UVC. That's why I have shielded the UVC so that the rays can cover only the portion nearer to the HEPA filter area.
MrRedBeard9 months ago
That is incredible
antoniraj (author)  MrRedBeard9 months ago
thank you...