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My interest in Mycology and Tissue culture started long back which led me to collect few laboratory equipment like Petri dishes, test tubes, weighing equipment, measuring glasses and also chemicals like Hydrogen Peroxide, Agar-agar and Potassium Permanganate. One equipment I wanted most but beyond my reach was a Laminar Flow Hood.

A Laminar Flow Hood provides a small sterile work area, where you can carry out all your culture works without the risk of contamination, simply by flowing completely purified air at a specified rate. However, the cost of the simplest form of the equipment is very prohibitive... the one with the very lowest specifications costs more than one thousand US Dollars... large corporations, universities, and research institutions can afford this but individual  hobbyists like me can not.

The alternative...? Construct your own equipment...

There are two main types of Laminar Flow Hoods : Horizontal Airflow and Vertical Airflow. In a horizontal type, the purified air is blown from the back and moves towards the front of the working area. In a vertical type, the air is blown from the top over the working area and moves out through the base

I have carried out extensive study and came out with a design for constructing a Horizontal type Laminar Flow Hood, making use of a vacuum cleaner for the blower and press board / Plexiglas for the hood body thus reducing the cost considerably.

This Instructable will provide you step-by-step instructions on how to build your own portable Laminar Flow Hood without any compromise on standard specifications.
 
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DamonA6 days ago
Simply Genius! Also like that you are emailing your instructable instructions. Please email me a copy too! damon.alverson@gmail.com
antoniraj (author)  DamonA6 days ago

please see attached files sent to your mail

Nice work! Could you please sent me the instruction files to prestond15@gmail.com? Thanks.

antoniraj (author)  ShamefulAunt12 days ago
Please go through the attached files sent to your mail

Please send detailed instructions to my email! nlabservives@gmail.com

Thank you for creating this.

antoniraj (author)  nlabservices17 days ago
please see files sent to your mail

Nice work! Please send your instructions to stumblingpiper@gmail.com

antoniraj (author)  stumblingpiper28 days ago
Please go through attached files sent to your mail
jotaceuy29 days ago

Excelent work! Please send instructions to my email jotaceuy@gmail.com. Thank you!!

antoniraj (author)  jotaceuy28 days ago
please see attached files sent to your mail
Doguehouse1 month ago

Please send detailed instructions to my email! Doguehouseinc@gmail.com

Thank you for creating this.

antoniraj (author)  Doguehouse1 month ago
Please see attached files sent to your mail
JeremyB151 month ago

I have been contemplating using a vacuum cleaner for a while, I have been assembling parts and I want to use one very badly for simplicity and cost. Everywhere, I read to use a blower from 400-600 CFM which seems like an overkill. Your vacuum is 42 CFM (20 LPS). My question is simply, does it work? Have you been contaminate free?

Awesome build man! You broke some rules, your a guy of my own heart. :-)

antoniraj (author)  JeremyB151 month ago

Why not...? For the hobbyist who need only a small sterile space to carry out his experiments, this is sufficient. The laminar flow hood should provide a clean work area with purified air blowing at the rate of 0.5 mtrs per second which we are getting through the vacuum cleaner. More over the air passing through the vacuum cleaner is pre-filtered and almost clean. Please see Step 1 where I made some calculations. If you already have a vacuum cleaner, restrict the hepa filter area matching with the air flow of your vacuum cleaner. 400-600 CFM airflow is for larger hoods used in laboratories.

Lastly, thank you for your appreciation...

I am starting my build, I will keep you posted. Its gotta be kinda neat for you to see how you inspired a microbiologist halfway around the world in Ohio, U.S.

Thank you for the inspiration.
smxles1 month ago

this is incredible! can you email the instructions via email at smxles@gmail.com

antoniraj (author)  smxles1 month ago
please go through attached files sent to your mail
MuhammadF301 month ago

Its a great invention! Can you please email me the instruction via email at m.fikri@outlook.my

antoniraj (author)  MuhammadF301 month ago
Please see attached files sent to your mail
EristalU3 months ago

Thank you for sharing! Will you please share your instructions via email with me as well? 2013.utest@gmail.com

Thank you!

antoniraj (author)  EristalU2 months ago

please see files sent to your mail

DevinF22 months ago

Aloha,

Would you be able to send me the copy of instructions as well? My email is devin.frederciicksen@gmail.com. Thank you so much

antoniraj (author)  DevinF22 months ago
Please see attached files sent to your mail
DevinF2 antoniraj2 months ago
Thank you for your response, however, I misspelled my email. It is devin.fredericksen@gmail.com. Would you mind sending the instructions again? Thank you
antoniraj (author)  DevinF22 months ago

ok, I have resent the files

skeletoniqbal2 months ago

such a great invetion sir, could you sent me the copy of instruction? these my email iqbalskeleton@gmail.com Thank you

antoniraj (author)  skeletoniqbal2 months ago
please see attached file sent to your mail

Hi Antoniraj,

I interested in this. Could you please email instructions to jsxventure@comcast.net

Thanks you!

antoniraj (author)  JimmyTHEWISEGUY3 months ago
Please see attached files sent to your mail
homopue3 months ago

Hi antoniraj,

very interested in your work.

Could you send me the instructions as well?!

Thank you!

homopue[at]gmail[dot]com

antoniraj (author)  homopue3 months ago
Please see attached files sent to your mail
evanjamesc3 months ago

Could you send me the instructions? evanarchiejames@gmail.com

antoniraj (author)  evanjamesc3 months ago
please see attached files sent to your mail
DerrickT33 months ago
cristofero4 months ago
Hi antoniraj..could you please send me plans for the flowhood? Did you have success with growing mycillium using the unit you made?
antoniraj (author)  cristofero4 months ago
please give me your mail id, I will send the construction details to you. I have not tried it as I got busy with other projects but you can use this for growing mycillium
TobeySynBio4 months ago

Hi AntoniRaj, I am also very interested. Can you please send me the instructions to tobyworldwide[at]web[dot]de ? I would appreciate that a lot! Thank you, Toby

antoniraj (author)  TobeySynBio4 months ago
Please refer to attached files sent to your mail
ahenrique45 months ago

Hi Antoniraj, I enjoyed so much your project, could you please send me the instruction for my adress? andrehenrique_bio@yahoo.com.br

Thank you

antoniraj (author)  ahenrique45 months ago
Hi ahenrique, please see the attached file sent to your mail
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