Picture of Portable Laminar Flow Hood

My interest in Mycology and Tissue culture started long back which led me to collect few laboratory equipment like Petri dishes, test tubes, weighing equipment, measuring glasses and also chemicals like Hydrogen Peroxide, Agar-agar and Potassium Permanganate. One equipment I wanted most but beyond my reach was a Laminar Flow Hood.

A Laminar Flow Hood provides a small sterile work area, where you can carry out all your culture works without the risk of contamination, simply by flowing completely purified air at a specified rate. However, the cost of the simplest form of the equipment is very prohibitive... the one with the very lowest specifications costs more than one thousand US Dollars... large corporations, universities, and research institutions can afford this but individual  hobbyists like me can not.

The alternative...? Construct your own equipment...

There are two main types of Laminar Flow Hoods : Horizontal Airflow and Vertical Airflow. In a horizontal type, the purified air is blown from the back and moves towards the front of the working area. In a vertical type, the air is blown from the top over the working area and moves out through the base

I have carried out extensive study and came out with a design for constructing a Horizontal type Laminar Flow Hood, making use of a vacuum cleaner for the blower and press board / Plexiglas for the hood body thus reducing the cost considerably.

This Instructable will provide you step-by-step instructions on how to build your own portable Laminar Flow Hood without any compromise on standard specifications.
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Hi AntoniRaj, I'm very interested in this project, could you please send me the instruction to this address?

antoniraj (author)  lorenzo.bewsenzio28 days ago

Please see the attached files sent to your mail for more information

Hello antoniraj, Can you please send me the instruction for a vertical type hood to my email

Thank you very much for your help. I think the world needs more people as you because you give real help.

thank you... Please see attached file sent to your mail for construction details of vertical type Laminar Flow Hood

tomn41 month ago

Sorry but this is a total waste of time and money. For laminar flow you need the proper hepa filter matched with the proper blower to achieve 100 cfm. Anything else wont proved true lamiar flow. Also pressurized glove boxes defeat the purpose and increase contaminants. The way a glove box works is to create dead air with no turbulence so the continents drop to the bottom after a spray with water.

antoniraj (author)  tomn41 month ago
I don't think you have gone through all the steps of this instructable fully.
ErnestM11 month ago

Very great read. love this article. would like to know more details. please send it at TIA!

antoniraj (author)  ErnestM11 month ago
Please go through attached files sent to your mail for more information
thebobr.3 months ago i'm preparing to build mine but i'm thinking if i could make few changes. For instance.

UV lighting. You can get cheap UV LED they should work just as good.

I'm thinking of making the whole body out of plexi glass and making it a bit smaller as first of all i have not a lot of space on my desk, second of all plexi glass is expensive in my local building supplies depo.

Now i could just use a vacuum cleaner, i may even have one somewhere in the attic but if to try push the cost down even more :) Do you think that this is going to work if I use different filter configuration...for example I already have HEPA filter that is going to be installed just like in your hood, but if I fix and additional paper filter on the entry pipe this should do the job shouldn't it?

What worries me the most is the actual vacuum cleaner, it seems...loud, not economical as it will consume a lot of energyand it will take a lot of spaces. I've been thinking if different motor can be used and if so what power would be suitable...For instance i've got this little electric air pump (used for kayak, mattrace etc) the airflow on it is 680 l/minute. it is 5 times smaller than a vaccum it can be made much more quite and it can work on batteries....

antoniraj (author)  thebobr.3 months ago

you can make any changes you want to the basic design. The aim is to get a sterile work area.

Regarding UV lighting there are many options available in the market. You can install UV LED lighting also, but make sure it is UVC.

To cut cost, you can use plexi glass in front and at top for better visibility.

If you have a HEPA filter available with you then a paper filter at the inlet will also do the job. But the problem is cleaning. In my design all particles are collected by the vacuum cleaner filter & it requires cleaning and replacing that filter only. The vacuum cleaner will also do the job of cleaning the HEPA filter by reverse flow of air.

Regarding the smaller blower instead of the vacuum cleaner, please carry out a little calculation with reference to the area of HEPA filter surface as I have shown in Step 1 of this instructable. If it is within limits you can use it.

hadrien.velge3 months ago

Could you send the instructions to ? Thank you very much!!

antoniraj (author)  hadrien.velge3 months ago
Please see files sent to your mail

Thanks for this great instructable! Can you please send the instructions to Thank you very much!

antoniraj (author)  sohospringfield3 months ago
please see attached files sent to your mail
Mycpdp3 months ago

Could you also send the instructions to please? Thank you in advance sir!

antoniraj (author)  Mycpdp3 months ago
please see attached files sent to you...
riaan.bothma4 months ago

This is great, exactly what I need, will you send instructions to please.

antoniraj (author)  riaan.bothma4 months ago
Please see attached files sent to your mail

Will you also please send me the instructions and details you used to build your Laminar Flow Hood to , I would love to build one of these and work with my orchid seeds inside this unit.

antoniraj (author)  shirley.bollinger.54 months ago
please go through the files sent to your mail on how to construct a laminar flow hood

could someone please send the details to

antoniraj (author)  conor.scully.545 months ago
please see attached files sent to your mail
dublinmask5 months ago

Thank you for sharing! Will you please share your instructions via email with me as well? thank you

antoniraj (author)  dublinmask5 months ago
Please see attached files sent to your mail
abeer gad5 months ago

its very useful please send me instructions my email is

antoniraj (author)  abeer gad5 months ago
Please see attached files sent to your mail for more information
Suhailc1236 months ago

This was a great instructable!

Sir can you send me too the detailed instructions

My email:

Keep up the good work and Thanks for sharing it with us.

antoniraj (author)  Suhailc1236 months ago
please see attached files send to your mail
Mallari07316 months ago

Sir can you send me instruction in building the laminar flow hood is my email address thanks in advance

antoniraj (author)  Mallari07316 months ago
Please go through files sent to your mail
nrajsathya8 months ago

Hello Sir, Can you please send me the instruction for a vertical type hood to my email id.,

Thank you very much for your help.

antoniraj (author)  nrajsathya8 months ago
please refer to the file sent to your mail id...
TylerM2 antoniraj6 months ago

Hello there sir! Could you send me more details at I appreciate this instructable greatly

antoniraj (author)  TylerM26 months ago

please go through files sent to your mail

akumar2041 year ago
Very much useful sir! I need to build one like this for mushroom spawn production sir, send me more details to Thanks in advance
antoniraj (author)  akumar2041 year ago
please go through the basics sent to your mail id
holly-g1 year ago
What's it used for, domestically?
antoniraj (author)  holly-g1 year ago
It is a laboratory equipment used for tissue culture, Mycelium culture in mushroom growing and used by scientists / research persons for other purposes also.. this provides an uncontaminated microorganism free work-space
carlos66ba1 year ago
Very well done! Suggestions: use fluorescent uvc bulb rather than incandescent for higher UV intensity and less heat; add a uvc bulb to the main chamber to sterilize as needed (normally should be off, and add a switch that turns that bulb off automatically if the gate is open). Lastly: is flexiglass sufficiently opaque to UVC?
antoniraj (author)  carlos66ba1 year ago
thank you carlos66ba... regarding your suggestions...

I have searched for a whole day in a big city near me and could not find any UVC bulb being sold by electrical shops.. I could lay my hand on this bulb through ebay India and at that time it was the only one available there. So, there was no choice for me.. I will replace it with fluorescent uvc bulb whenever I can find them. I will add this to the main chamber.

I think adding a pressure operated switch with the front opening will automatically turn off the uvc lighting whenever the gate is opened. it can be very easily done.

Lastly, though there are many suggestions, I don't think plexiglas is sufficiently opaque to UVC. That's why I have shielded the UVC so that the rays can cover only the portion nearer to the HEPA filter area.
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