UV-transilluminators are used in molecular biology labs to view DNA (or RNA) that has been separated by electrophoresis through an agarose gel. During or immediately after electrophoresis, the agarose gel is stained with a fluorescent dye which binds to nucleic acid. Exposing the stained gel to a UVB light source causes the DNA/dye to fluoresce and become visible. This technique is used wherever the researcher needs to be able to view their sample, for example sizing a PCR product, purifying DNA segment after a restriction enzyme digest, quantifying DNA or verifying RNA integrity after extraction.
This Instructable tutorial describes how to make a UVB (310nm) transilluminator with a 7 x 7 cm window for viewing ethidium bromide (or SYBR-Safe) stained DNA mini-gels. Once all of the materials are collected, the actual assembly time is approx. 1-2 hours. Some soldering is required.
For the UV transilluminator enclosure and lid, download the design file attached below (svg or PDF file). Laser cut the parts from the material listed in the design file. If you do not have access to a laser cutter, you can send the files to any laser cutting service such as Pololu. Materials for laser cutting can be found at any supplier of acrylic materials (McMaster-Carr, US Plastics etc) except for the solacryl (UV-transmissive) which can be bought from Loop Acrylics.
Tap holes in the following parts:
A video of hand-tapping acrylic can be found here: https://www.instructables.com/id/Gel-electrophoresis-system-mini/step2/Comb-assembly.