Crude Hologram Like Effects With Lasers and Glowing Dyes (with Prisms).

Introduction: Crude Hologram Like Effects With Lasers and Glowing Dyes (with Prisms).

About: I am a part-time hobbyists biotechnologist with a photonics background. I also have a YouTube channel too. I work part-time too.


I am making a crude type of Hologram, not a true type Hologram by using a 100-200 mw Purple 405 nm with the following dyes (Pink Magic maker- Rhodamine B, Sodium fluorescein, Tonic water- 80 mg per L Quinine sulfate, Palmolive soap, and Tide HD soap 10 ml in 50 ml of water).

I will be showing how to extract the Rhodamine B from Pink Markers with Pliers and a small tree cutter to remove the lining of the marker with the substrate medium and the dye.

Note that true holograms use lasers and very expensive mirrors and beam splitters. A person probably cannot afford a 2000+ dollar true beam splitter for home use. I mean on eBay you can find decent beam splitters for lasers but I can not find 405 nm or ones for different wavelengths to make a hologram with multiple dyes. You would for a true hologram show use a 5 mw to 10 mw laser Not a high-powered laser at 100 to 200 mw.

Warning: These dyes are Irritating and do stain. The lasers at 100-200 mw can burn things and you need good safety goggles for 405 nm. I recommend some good 62 dollar laser safety goggles. They can cause irreversible blindness and set matches on fire.

They say OD 6 for 400 nm or SO but People have measured it at 2.5 OD. For a 250 mw Purple laser and different beam outcomes, this would be effective at reducing the indirect beam around 200-250 mw max. If u are working with 500 mw Laser beams you need 200 dollar good glass laser beams. These are fine for Class 3B not Class 4 lasers.


Normal supplies.

7 by 7-inch Magnetic stir bar they can be 100 dollars Normal Price.

High Powered laser Module or Pointer 80 to 200 mw, Max.

Prisms 20 dollars on Amazon. 6 cm, 1 cm, 2 cm (L, W, H).

Beakers 100-200 ml, Big size, Normal big Jar Jams.

Stir Rods small and big 0.5 cm, to 2 cm.

Digital scale and Potentiall

Stands and clamps to hold lasers and other stuff.

Bricks to hold the clamp.

Sink to wash out solutions and magnetic stir bar retriever.


Sodium fluorescein 99 percent- eBay, Amazon, Or Lab suppliers.

Rhodamine B, (eBay 50 g for 50 dollars Russia fed),

Or Pink Magic Markers from or

Tonic water (Quinne sulfate 80 mg a L). Any local store.

Tide HD soap, Any Pharmacy, etc.

Palmolive Soap (Dish detergent type).

Step 1: Theory of How Crude Holograms Work and Where Get Lasers.

Mirrors and a magnetic stirrer and hot plate are used. A powerful laser 100 mw to 200 mw is fired into a beaker which causes the dyes to glow in different colors. If you move the dyes manually with the beaker the laser beam shines on the Aluminum foil or other stuff and reflects the light off the beaker onto the Aluminum foil this also works with the 10 X, 7-inch focusable Mirror.

My laser has dropped down to 80 mw so it is weak I am waiting for a 200 mw powerful 405 nm laser.

It is also important to run the laser with a duty cycle in mind. My old laser was 20 minutes On, 3 minutes off this cools the laser down and will protect the laser from prematurely burning out. If your laser does not contain a fan to it, you may need to cool it with a small fan.

The laser is reflected onto three mirrors or two mirrors (Two mirrors onto the side of the table 15 cm away from the stirrer. With one of my projects, the laser was shinning 90 degrees flat on the rod a few cm away from the prism.

Here some videos on Lasers and wavelengths. You could also use a low powered 100-200 mw laser pointer with a 3.7-volt lithium battery with Recharger. It how to get the lasers and some info.

eBay I recommend Laser Land type lasers.

Push-button lasers I do not recommend since it will be impossible to align and turn on and keep the laser on. This is also dangerous the laser could easily burn out if you taped the switch. (Laser 303 Purple 405 nm burning laser.)

Step 2: Preparing the Dyes. Fluorescein and Rhodamine B. Part 1.

Sodium Fluorescein dye. You can get it from Magic markers by adding water or ethanol, etc to a 600 ml beaker and prying open the dye's content. You then squeeze while wearing gloves and PPE the dye out of the marker liner. Each marker contains 10 ml of 1 percent of the dye.

My picture show how to remove both the Yellow markers and the Pink Markers from their container. This is cheap to do 2 to 3 dollars for 2 markers in 100 ml of water. You could filter it and boil it down but I choose to keep it as a solution (as is.).

You can add 100 ml of ethanol or Isoproponal Alcohol.

If you have magic markers that are yellow you can extract them in this video. Or you can buy Sodium fluorescein 50 g on eBay for 60 dollars yes it is quite expensive. You only need a few grams of the sodium fluorescein chemical in water.

You could boil them down with ethanol in a well-ventillated area to get the crystals of Sodium fluorescein or buy the crystals and add the water to the 1 g of the crystals. Weighing out the crystals.

Step 3: Making Dilute Tide HD Soap and Palmolive Soap Solutions. Part 2.

Here I am adding 30-35 ml of Palmolive soap (Dishwasher) type to 100 ml of water with 100 ml of Tonic water the video will show how it can be quickly done.

I am not sure what the glowing dye is in Palmolive soap detergent? If anyone knows can they make a comment so I can research that. I know Tide HD is a Stillibine chemical.

Step 4: Making Tide HD With Stilbene Chemicals.

In order to make a diluted glowing sample, you add 100 ml Tide HD for Washers to 150 ml of tap water I will take more pictures and post them here.

I cannot give a lot of info here since it is very simple your mixing 100 ml tide with 150 ml water. The stilbene chemical can be quite low 1-2 percent average. So 100 ml in 150 will give you 1 percent times 100/250 = 0.4 percent Stillibine to 0.8 percent.

Step 5: Laser Holograms With Mirrors (Palmolive Soap and Tide).

We put a paper 11 by 11 inches with an adhesive sticky side of the Plastic mirror on the paper and it will adhere very quickly nearly instantly. Here the next step.

Under a 7 by 7 or greater Magnetic stirrer or Hotplate (Not using the Hot plate function for this Project), we have with the stands the laser as flat and low as possible with the stand and clamp.

Here the type of clamp I am using...

The prism is located a few cm from two beakers with a min stir rod.

Step 6: Pink Magic Markers and Sodium Fluorescein Dye Hologram.

In this case, we have the convex mirror right in front of the Hot plate about 10 cm above the hotplate. A mirror a fake Mirror made from Aylcric is placed underneath the hot plate or Magnetic stirrer (Or both).

Depend on what dye you are using you, may want to put the dye in a plastic bottle (Alcohol bottles) to store them. Keep them in a dark area away from light or heat.

Have the mirrors One directly in front of the Mirrors in front of the Mirror and one left or right of the Hot plate-Magnetic stirrer, etc. This will produce a crude type of hologram.

Step 7: Some Final Steps.

Here I will try to explain the hologram effects of having a 5 inch to 7-inch mirror and having a laser shine into a prism at low levels. My clamps are normal 1.5 foot in length good quality clamps with bricks to hold the clamp from falling into the beakers with (Lasers 404 nm, 100-200 nm) into the solutions.

Note that the prisms are fragile and great care in not breaking them is required. If they fall they will shatter into a million pieces. They are quite cheap.

Make sure to keep the lasers at an angle that is flat and do not be eye level with the laser this is dangerous you could get an Indirect hit from the laser beam. Laser safety is VERY IMPORTANT.

Also You can use centrifuge tubes 50 ml and add dye glowing dye to it and get the solutions to hang on a wire over a 600 to 700 ml beaker. You can add a stir rod 0.5 cm one to a solution and center it evenly. A prism could be added to it and you can add different dyes to it.

Step 8: Final Conclusions.

These types of Holograms with glass and Mirrors and plastic mirrors on a hotplate with Paper or Aluminum foil is very interesting Project.

As I said normal holograms use expensive beam splitters and are usually one type of laser and one color, not multiple colors as explained with the dyes here. Rhodamine 6B is an another option but you need to extract and filter it with only Isoproponyl alc.

Holograms on Wiki.

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    1 year ago

    You can also add Phosphorous materials to tubes that under Pulsing and regular UV light glow different colors. Glow in the dark Materials 30 ml you only need 1 ml of each tube.


    1 year ago

    Rhodamine B with 4 ml of 4 g of Ammonia carbonate in 1 L of tap water with 405 nm and 532 nm laser light. Just some ideas the base shifts the Rhodamine B which depending On the OH- or H+ forms two isomer molecules.


    1 year ago

    With the laser on the beaker and the plastic sheet removed this is what the tube effect of laser beam on 6 tubes is. Yellow= Sodium fluorescein, Pink = Rhodamine b salt, Dark blue= Stibline chemical in dish detergent, Green = Palmolive soap and Light blue tonic water.