How to Make Fluorescein From Highlighter Markers

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Introduction: How to Make Fluorescein From Highlighter Markers

About: I like turning boring things into awesome things! Usually on video.

The above video demonstrates how to extract the chemical fluorescein from highlighter markers using no other chemical besides common rubbing alcohol. As is demonstrated in the video, fluorescein is spectacular to watch dissolve into water, especially under black light. Below is a list of other excellent videos that feature fluorescein.

Dr. Nozman's demonstrations (subtitled in English):
http://youtu.be/iQlE1dKJmy4
http://youtu.be/BRBc3C6R-DA

HouseHoldHacker:
http://youtu.be/s8dctujbP2s

NurdRage (An excellent tutorial to make Fluorescein in the lab):
http://www.youtube.com/watch?v=ihssISvNL3o

In addition to the above videos here are several links describing the process by which Fluorescein is used to detect trace amounts of blood:
http://www.crime-scene-investigator.net/fluoresceinmethod.html
http://www.latent-prints.com/fluorescein2.htm

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    31 Discussions

    0
    amjy88
    amjy88

    7 years ago

    Can this be done with other highlighter colors or just yellow ones?

    0
    NightHawkInLight
    NightHawkInLight

    Reply 7 years ago on Introduction

    Only yellow ones will have fluorescein, but other colors may also use somewhat interesting dyes.

    0
    JoselinaL
    JoselinaL

    Reply 4 years ago

    What are controlled variables in this experiment??

    0
    clachancebrais
    clachancebrais

    Reply 6 years ago on Introduction

    What dyes exactly are contained in other highlighter colors?

    0
    eerambach
    eerambach

    Reply 7 years ago on Introduction

    How does the red/green fluorescein get used in yellow highlighters?

    0
    NightHawkInLight
    NightHawkInLight

    Reply 7 years ago on Introduction

    It changes colors depending on the concentration and the angle that it's looked at in certain lighting. If you have a test tube of the concentrated liquid one side will look yellow, the other red as you hold it up to a light. It's weird stuff.

    0
    pg1315
    pg1315

    7 years ago on Introduction

    so since you said the Fluorescein is nontoxic, does that mean i can mix it in a drink to make it glow under a blacklight?

    0
    NightHawkInLight
    NightHawkInLight

    Reply 7 years ago on Introduction

    It's non toxic, but from what I've read it does make you nauseous if you ingest it. There also might be some other chemicals in a highlighter that might not be as safe. To make drinks glow, mix them with club soda. It will glow blue under black light.

    0
    Yonatan24
    Yonatan24

    Reply 4 years ago

    Do you mean "Tonic Water"?

    Or is it the same thing?

    0
    HavocRC
    HavocRC

    6 years ago on Introduction

    How long will it last? Like if I put it in a bowl of water, will it begin to fade after a few days?

    0
    mymyjames2000
    mymyjames2000

    7 years ago

    Is it possible just to squeeze the inner tube instead of messing with the bottle and alchohol?

    0
    gassie202
    gassie202

    7 years ago on Introduction

    Just a tip, if you want to obtain fluorescein powder, you can use the following steps:
    1. obtain a flat glass surface, pour the flourescein on it in batches small enough that the surface tensoin keeps it together.
    2. Use a hairdryer on highest heat at the lowest speed. watch out that you dont hold the hairdryer to close because je might blow the solution everywhere.
    3. keep this up untill the fluorescein dries up and sticks to the glass.
    4. use a flat metal, glass or plastic object like a razor or old credit card to scrape all the fluorescein off the glass and collect it in airtight pot.

    A glass surface is not a must, but the powder is a lot easier to see. So you wont get fluorescein anywhere you don't want it.

    Nice tutorial btw, had no idea markers contained that much of the stuff.

    0
    Gravity Boy
    Gravity Boy

    7 years ago on Introduction

    Would this florescence with an AC component around it? Meaning could you mark an electroluminescent wire or plate using capacitance?

    0
    Undwe
    Undwe

    7 years ago on Introduction

    highlighters have never been more interesting

    0
    dmb321
    dmb321

    7 years ago on Introduction

    awesome I will no longer see highlite markers the same way!