How to Grow Oyster Mushrooms (Low Tech)





We have been introducing mushroom cultivation as a nutritional supplement and cash crop for the landless poor. Oyster mushrooms are a high yield, fast growing crop. They are known to help lower cholesterol levels and are a great source of potassium, iron and protein.

This instructable gives a low tech, step by step guide to growing both pleurotus ostreatus (winter strain) and pleurotus pulmonarius (summer strain). Oyster mushrooms are highly tolerant of variations in temperature, humidity, light levels and carbon dioxide levels, making it a great choice for first time growers.

See related instructable - How to Grow Oyster Mushroom Spawn (Low Tech)

Step 1: Materials

You will need...

Straw (the medium for growing the mushrooms in)
Containers (for soaking straw)
Plastic bags (or reusable containers for holding straw)
Elastic bands or string (to constrict bag opening)
Cotton wool (to filter out contaminants)
Barrel or drum (for pasteurising the straw)
Material liner (for holding bags within barrel)
Gas burner (for heating barrel)
Bleach spray (to clean growing room)
Spoon, gloves, clean clothes, face mask (to look the part when inoculating straw)
A growing area that can retain moisture in the air, shaded with some light
Possibly plastic sheeting (to help retain humidity & to reduce other unwanted moulds)
Mushroom spawn (see How to Grow Mushroom Spawn)
A water or weed sprayer (to increase humidity within growing room)
A thermometer and hygrometer (to keep an eye on temperature and relative humidity)

Step 2: Soak Straw, Drain and Bag

The mushrooms require a medium to grow in, in this case we will be using straw. The straw length should be approximately 5-10 cm (2-4 inches). Placing the straw in water tight containers, submerge the straw in water for 24 hours. Wash, rinse and drain thoroughly, then bag in 5 litre plastic bags ready for pasteurising.

Step 3: Pasteurise

Position your drum onto the heat source (we used a gas burner), pouring around 40 litres of water into the drum. Place a suitable platform at the bottom of the drum, one that will keep the bags above the water yet allow steam to rise. Insert a material bin liner and fill with the prepared bags of straw. Close off the bags with the liner and cover the drum with a lid. Heat the drum, steaming the bags for approximately 60 minutes. It should take around 30 minutes for the steam to make its way to the top bags (the temperature should near 95°C ~200°F). Leave to cool, removing the bags and transferring them to the growing area.

Step 4: Prepare Growing Room

The growing room should be clean and dimly lit (shaded with indirect sunlight), able to retain moisture in the air yet also provide an airflow when ventilation is needed. Plastic sheeting can be used to seal off an area to help retain humidity and to reduce other unwanted moulds and insects.

To prepare the room for the inoculations, spray a 1:20 (5%) solution of bleach along walls and corners (any area where mould might like to grow).

Temperatures of 10°C to 24°C (50°F to 75°F) for pleurotus ostreatus (winter) and 10°C to 30°C (50°F to 85°F) for pleurotus pulmonarius (summer) should be available depending on stage of growth (initial spawn run, colonisation, pinning and fruiting).

Step 5: Inoculate Bags

Before inoculating the bags of straw, make sure you have showered and are wearing clean clothes. Clean your hands with antibacterial soap or wear sterile gloves. A face mask and hair cap will also help reduce contamination (we are very dirty creatures).

Open the bags of straw and the mushroom spawn. Taking a sterile spoon, place a few spoonfuls into the straw, breaking it up and mixing lightly. As a general rule, the more spawn you add, the faster the substrate will be colonised (with 1 litre of spawn, we inoculated about 10 bags - you could inoculate more).

Restrict the opening of the bag by placing a rubber band (or cord) around the bag's neck. Taking a small piece of cotton wool, plug the bag's opening to reduce the chances of contamination and insect infestation. Leave to incubate.

Step 6: Encourage Colonisation

Once inoculated, the bags should be left to incubate. During this time the spawn "runs" (mycelium spreads) throughout the straw. The spawn run will be complete when the mycelium has spread entirely throughout the bag (the straw is then fully colonised).

Depending on the mushroom variety, humidity and temperature, this process should take between 1 to 3 weeks.

Pleurotus ostreatus (winter), 24°C (75°F) 2 to 3 weeks
Pleurotus pulmonarius (summer), 24°C to 30°C (75 to 85°F) 1 to 2 weeks

During incubation, light is not required, however, make sure the bags have plenty of fresh air.

Step 7: Monitor Bags

It is important to monitor the bags for any sign of unwanted moulds and pests. While the straw is still in the bags, you shouldn't have a problem with insects or mice. However, the best policy for fighting both contamination and infestation, is prevention. You may want to spray some surfaces to deter flies and other insects from setting up home, mesh any windows and keep doors closed.

Regularly check bags for any mould contamination and remove any infected bags from the growing area. Black mould found within the straw may indicate ineffective sterilisation. You may also notice sprouting straw and the appearance of unwanted mushrooms such as the ink cap (see pictures). Green moulds are common and can be caused by contaminated spawn (ineffective grain sterilisation), high moisture / low spawn levels and ineffective straw sterilisation. At this early stage, it is better to simply remove infected bags, as you want to prevent its spread. Up to a 10% loss due to contamination is generally regarded as acceptable.

Finally, as the bags become fully colonised, the initial stages of fruiting (or pinning) may be seen.

Step 8: Encourage Pinning

Once pinning has started, it is time to remove the substrate from the bags. Pinning naturally occurs as humidity increases, low levels of light appear and temperature levels fall. Increase the growing room humidity by regularly spraying with a water sprayer (avoid spraying directly on the mushrooms). You can also wet the floor and leave open containers of water in the room (95-100% humidity is recommended). As our climate is very dry, we only managed 60% at best, dropping down to 40%, by spraying 5 litres of water 2 - 3 times a day (even at these humidity levels a good result can be achieved). To prevent excessive CO2 levels, allow the growing area to flush with clean air before spraying. If you can, regulate the temperature accordingly.

Pleurotus ostreatus (winter), 10-15°C (50-60°F)
Pleurotus pulmonarius (summer), 10-24°C to 30°C (50-75°F)

You may notice an initial drying out of early stage pinning, as you remove the plastic. As you maintain the humidity levels this will regenerate. Keep a close eye on flies and spray when needed. If any mould is found, either remove the infected straw or the entire mound from the growing area.

Step 9: Harvesting

As the mushrooms begin fruiting, it is important to keep the humidity high (85-90% is recommended). As before, allow air to flush through the growing area prior to spraying (oyster mushrooms require a consistent source of fresh air). Temperatures can now be higher than for the initial pinning stage.

Pleurotus ostreatus (winter), 10°C to 20°C (~50°F to 70°F)
Pleurotus pulmonarius (summer), 16°C to 28°C (~60°F to 80°F)

Remember to constantly monitor for pests, such as flies and mice, as they can quickly ruin a crop. You should expect three or more crops, each taking around a week or so to mature. You may harvest the mushrooms at any size, however, once a mushroom has reached its full size, you will notice it will begin to dry, turning a yellowish colour (they taste great, even dry). When harvesting, remove the mushroom completely, by twisting firmly at its base. After harvesting a few crops, we found it helpful to stack the mounds of straw, which seemed to help increase the yield. If you find your mushrooms with long stalks and small caps, they may not be getting enough light, also high CO2 levels can also lead to small deformities (allow for more fresh air). After the straw ceases to produce mushrooms, it can be fed to livestock or composted.

Now, finally take your harvested mushrooms and create a delicious mushroom meal. Enjoy.

See related instructable - How to Grow Oyster Mushroom Spawn (Low Tech)

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113 Discussions


7 weeks ago

Very interesting post. Beautiful described
each and every step of oyster mushrooms cultivation. This post is helpful for
many individuals. Thanks for sharing this information subject to us. I am a
businessman and I am running a mushroom farm. I suggest, before going for any
type of mushrooms farming, the first and prior thing is the organic and the
real products used in farming. I purchase a mushroom kit from
They provide materials and supplements for mushroom farming which is real
organic and finished products.


Question 4 months ago

Hi I'm a beginner my bags got attacked by rodents during incubation period, where openings got created on them, it took 2-3 weeks for some to get colonized while others failed to colonize so after I exposed them to growth conditions and watering 2 times a day, tell me will I get good results and how long will need to see fruiting happen.. Thanks from Uganda


Question 9 months ago

I am a beginner. I am based out of Solan, Himachal Pradesh, India. I started trying to grow oyster california variety as the temparature here is around 15 to 20 deg Celcious. I am trying to grow on a bag of wheat straw. I bought the spawn from a very reliable place and pasteurized my straw as guided by cooking it at about 60 to 70 deg c for 1 hrs. After this I tried my straw till they were at room temp around 20 deg c. Then stacked it in a polythin bag alternating with straw and spawn upto 3 layers. I tried to tightly pack an remove the air from the bag before closing the mouth. I then pinned holes for air to go. I kept the bag in a shady area indoors with good air circulation. I found good Mycelium growth in about 15 days. I then made some bigger cuts in the bag for primordial growth. Today is fourth day after cutting bigger openings. I am spraying water 2 to 3 times but no pin heads can be seen yet. Please help.

1 answer

Answer 8 months ago

All your process is correct..but at last portion you made some mistake. don't cut big holes in the bag cause initial pinning is begin through the holes you made at spawning. Secondly don't spray too much water cause it carries enough moisture in the bag to pinning for first time. If mycellium collonization is completed give some fresh air...let's see the result..I hope it will help.


11 months ago

Hi Mr. rocket surgery :)

I'm a beginner and you have no idea how happy and excited I am to have come across your articles on spawn and mushroom growing.Just a couple of questions, though.Is possible to use lemon grass or banana leaves as substrate,instead of straw?Do I only need one kind or mix two kind of substrates?Having only a small area at home, can I use a plastic container box for my fruiting bags while doing the usual procedure ( leaving the lid open for fresh air, spraying the area inside, then closing afterwards)?

Looking forward to your reply.Thank you!


Tip 1 year ago

This one i made in my college days..


Question 1 year ago on Step 8

if removing the plastic bag entirely. How do you keep the straw from drying out, ( mine is in the kitchen ) I did this before and the substrate dried out very quickly, so I ended up covering it in cling film ?

1 answer

Answer 1 year ago

Basically, you can do either. You can wait until your mushrooms begin to pin (they should pin and even pinning through the plastic) and then remove bag. This method will require you to keep the humidity surrounding the medium around 85-90% (keep the growing area enclosed and spray regularly, flushing with fresh air occasionally). Otherwise, you can make cuts in the growing bag where you will find the fruiting will occur. This certainly helps with regard to keeping the medium moist but I find having the entire bag removed within a humid growing area seems to help with your yield. You should get three good flushes. All the best, try some things out to see what works best for you.


Question 1 year ago on Step 5

hi. I too am a beginner, when the bags are in a dark room being colonised, have you put any slits in the bag so the mushrooms can escape or does this come later when you expose them to oxygen and light ? Xx


1 year ago

I'm a beginner here and was wondering if I would have to completely restart a different bag after the harvest or if the oysters fruit multiple times before being exhausted. Thank you for any help and tips.

1 reply

Reply 1 year ago

Hey there, See "Step 9: Harvesting" - "You should expect three or more crops, each taking around a week or so to mature. You may harvest the mushrooms at any size, however, once a mushroom has reached its full size, you will notice it will begin to dry, turning a yellowish colour (they taste great, even dry). When harvesting, remove the mushroom completely, by twisting firmly at its base. After harvesting a few crops, we found it helpful to stack the mounds of straw, which seemed to help increase the yield. If you find your mushrooms with long stalks and small caps, they may not be getting enough light, also high CO2 levels can also lead to small deformities (allow for more fresh air). After the straw ceases to produce mushrooms, it can be fed to livestock or composted."

All the best!


1 year ago

sir I'm trying to grow Sajor caju at Lucknow up. After mycilium run of 25 days I exposed bags to light and made an arrangement with cooler to maintain temp between 27 to 30 deg. 19 days are passed ,till now pinning had not occured, will the fruiting occur, pl help?


2 years ago

my name is Seyoum, I have been working on oyster mushroom cultivation. and I have so many questions, like
1 how much water I need to spray on the mushroom after growing?
2 how much air it needs?
3 how to prevent flies(tired of killing them by hand)


2 years ago

I am Saurabh Bhatnagar, Very recently took a days training on Mushroom cultivation.
Started doing it at home. I am able to form beds properly , with very good mycelium growth but further pinning or fruiting happens . Why?
I have room of 12 X 14 fts.. Only one portion I am using for cultivation & inoculation. Two side doors to it. One door opens in balcony & other inside the house to other room corridor. I placed 3 Bamboos in parallel at the height of 8 Fts. the area is covered with Thick gunny cloths. I pour water over it to maintain humidity. So fruiting area is about 4 fts X 10 Fts. Covered with gunny cloths one side & other side is wall. Placed at gunny cloth on the door which is opens in balcony and keep other door closed so that humidity level maintains.
Room temp. close to 21~26 degree centigrade . Humidity 60~85%
Morning --
I open the gunny cloths from the bamboos for 3~4 hrs to allow fresh air come in fruiting area. Spry very less water to all the bags.

Could you pl. guide me where I am going wrong and not able to achieve fruiting.
What r the factors effective fruiting / yield


2 replies

Reply 2 years ago

A week after your substrate is fully colonised you should start to see some pinning. In nature you get a drop in temperature with Spring rains, so you can sometimes (depending on your variety) place your substrate in a cooler area and then bring them back after pins start forming. Test your substrate is fully colonised and all smell the bags (healthy bags should smell sweet).


Reply 2 years ago

Thanks for your reply,

My problem is why after very good pinning it dry out . Heads are not grown?

spraying water 3~4 times a day to maintain humidity. Temp. is about 28~32 degrees. Guy cloth curtains ( Half or the height) are hanged near to the fruiting area. Due to gunny cloth light is very less during day times also. do I need to open tube light in the room.


2 years ago

Hello Mr. Rocketsurgery,

You helped me earlier also when I started growing oyster mushroom. Now, again I need your expert advice in an out of the track question about Mycelium and oyster mushroom.

Q. Is it possible to stop mushroom fruiting completely after the spawn run is complete? I am somehow trying to stop fruiting once the spawn run is complete and the bag is completely white with mycelium. Is there a way to freeze this stage? Even the pinheads should not appear ever at any temperature and in all moderate environmental conditions for fruiting. I want the process to stop and sustain at mycelium run completion stage.

Please let me know if its possible somehow. It would be a great help here.



Surinder Verma


1 reply

Reply 2 years ago

I got it Mr. Rocketsurgery.

Thanks for your time. :)

I will get back to again for some other help.


Surinder Verma