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Lobster Elite Ball Machine ...View Instructable »
It doesn't really matter. With the grain will make it a bit tougher to tear off. My last few batches I have used Eye of Round steaks (They are nicely cut at about the right thickness for slicing into strips) and the grain ends up running cross-ways to the length of the strip of jerky. In my opinion, it makes it a bit easier on the teeth when eating.